Objective To observe the effect of general anesthesia induction with fentanil on parturient undergoing cessrean section and Apgar score of newborn. Methods Forty-two example cesarean section under general anesthesia were induced with fentanil 4μ/kg, propofol 1.5-2.0 mg/kg, atracurium 0.5mg/kg.Observed the change of hemodynamics parameter of parturientand 1 min,5 min Apgar score of newborn. Results Parturient woman blood stream dynamics Was stable. The premature delivery, the low body weight, the polyembryony or the anaesthesia had used the hypnotics group, its newborn 1 min Apgar score was obviously lower than that had not merged above situations(P＜0.01),but the newborn 5 min Apgar score was not significantly different(P＞0.05).Conclusions Fentanil used in cesarean section general anesthesia induction, parturient woman blood stream dynamics is stable, affects not obviously to newborn's Apgar scoure. But regarding the premature delivery, the polyembryony, the low body weight or the pregnant woman used the hypnotics, cegaresn section general anesthesia induction with fentanil should be supposed to use cautiously.

AIM: To establish the method for quality control of Chansu Tongbi Ointment(Venenum Bufonis,Borneolum Syntheticum,Radix Stephanial Tetrandrae etc.) METHODS: Radix Stephaniae Tetrandrae in Chansu Tongbi Ointment was identified by TLC,and the content of cinobufagin and resibufogenin were determined by HPLC. RESULTS: Radix Stephaniae Tetrandrae could be identified by TLC.Cinobufagin showed a good linear relationship at the range of 1.15 ?g-5.75 ?g,r=0.999 7.The RSD of measurement precision test was 1.05%,The average recovery was 97.95%(RSD=1.63%,n=5).Resibufogenin showed a good linear relationship at the range of 1.05 ?g-5.25 ?g,r=0.999 6.The RSD of measurement precision test was 1.02%,The average recovery was 97.45%(RSD=0.61%,n=5. CONCLUSION: The method is accurate,reliable and available with a reproducibility and can be used to control the quality of this ointment effectively.

Objective To study the synergistic antitumor effect of PCF when combine used with CTX.Methods Inoculated 0.2 mL H22 tumor fluid into the right armpit of mouse,and then the animals were divided into seven groups randomly: control group;CTX group(CTX 10 mg?kg-1);three test group(PCF 1000,500 and 250 mg?kg-1) and two combined use groups (PCF 1000,500 mg?kg-1with CTX 10 mg?kg-1).PCF were given by intragastric administration for 10 days,and CTX by intraperitoneal injection for 10 days.Same volume of saline was given to the control group.The mice were killed 24 hours after the last medication and the tumor inhibition rate was calculated.Results Three PCF groups had no effect on tumor inhibition,while two combined treatusement groups showed significant effect on tumor inhibition,while at the same doses the tumor inhibition rates were raised to 55.9% and 52.9% respectively,which were higher than that of CTX when used alone.Conclusion PCF can enhance the antitumor effects of CTX.

Objective To investigate the methods and efficacy of coronary stent placement in patients with myocardial bridge. Methods 4 patients with angina and ST-T changes in ECG were involved in the study. Coronary angiography showed there were 75%～90% systolic stenosis of LAD in all patients. Two of them were regarded as isolated myocardial bridge, and the others were regarded as myocardial bridge associated with atherosclerosis. The length of artery pressed by myocardial bridge was in the range of 10～18 mm. All patients who had repeated angina accepted stenting therapy. One of the stents was Crossflex, three were Nir stents. Stents size: (3.0～3.5)?(12～20)mm. All stents were expanded with 8～10 atm for 30～40 seconds. Results After stenting, coronary angiography showed no residual stenosis. In the follow-up 6～18 months there were no complaints of angina. In two of them, coronary angiography showed there were no restenosis after one year.Conclusion Coronary stenting is an optional method to treat patient who have myocardial bridge and have no efficacy after taking medicines, especially for the patients who have both myocardial bridge and atherosclersis. But futher study in large populations is still necessary.

Objective To evaluate the middle and short-term effects of integrative cognitive training on mild cognitive impairments(MCI).Methods All the participants with MCI aged over 70 years were selected from one sub-district of Putuo District,Shanghai and divided into cognitive intervention group (n=46) and control group (n=35).The integrative cognitive training was conducted in 24 sessions for 12 weeks.All subjects were assessed by Neuropsychological Test Battery for Elderly (NTBE) and by a questionnaire of Shanghai Health Survey for the Elderly (VER2006)at baseline,follow-up and one-year follow up phases.Results At baseline phase,semantic relations test in NTBE was better (t=2.13,P=0.037),verbal fluency test (vegetables) in NTBE was worse(t =-2.25,P=0.038) in intervention group than in control group,while there were no significant differences in scores of other neuropsychological tests between intervention group and control group(all P＞0.05).At follow up phase,16 subscales in reasoning test of NTBE were improved (t=-2.70,P=0.010)and 2 subscales were declined in cognitive intervention group(all P＜0.05).9 subscales in semantic relations test of NTBE (t=-2.27,P=0.013) were improved and 5 subscales were declined in control group (all P＜ 0.05).Comparison between groups at one year follow-up showed that 5 subscales in reasoning test of NTBE (F=16.80,P=0.000) were better in intervention group than in control group(all P＜0.05).General linear model (GLM) with repeated measures at three time points showed that 4 subscales in reasoning test of NTBE had a time-by group interaction (F=5.16,P=0.01).Conclusions Integrative cognitive training can improve cognitive function in patients with mild cognitive impairments,and the validity of reasoning ability can sustain one year.

Objective To detect the genetic amplification and protein expression characteristics of aurora-A in ovarian cancer and to interpret the role of aurora-A gene in course of onset,progression and regression phases of ovarian cancer.Methods The amplification of aurora-A gene was detected by quantitative PCR in 6 normal ovarian tissues and 8 ovarian cancer samples,and its protein expression was examined by immunohistochemistry in 6 normal ovarian tissues and 40 ovarian cancer samples,furthermore,the relationships between over-expression of aurora-A protein in ovarian cancer tissue and its pathologic classification,tissue differentiation,clinical phase,tumor proliferation trait and prognosis were analyzed.Results Quantitive PCR showed that aurora-A mRNA was significantly higher in 8 ovarian cancer samples than that in normal ovarian tissues(P0.05).Conclusion There are abnormal amplification and protein over-expression of aurora-A in ovarian cancer tissue,aurora-A probably play an important role in the onset and progression of ovarian cancer,and the novel biological treatment concerning aurora-A gene and its protein is probably a useful route for curing tumor.

Objective To analyze the literature distribution and research status of depression in the elderly published in Chinese Medical Association journals.Methods The literatures of elderly depression published in 85 kinds of Chinese Medical Association journals from 2003 to 2012 were collected.The publication year,distribution,content,author,institution and region of these literatures were analyzed.Results From 2003 to 2012,there were 85 articles on depression in the elderly published in 15 kinds of journals of the Chinese Medical Association.These literatures were published mostly in 2011 (18 articles,21.2％),secondly in 2010 (13 articles,15.3％) and 2012 (12 articles,14.1％),which were published mostly in Chinese Journal of Geriatrics (29,34.1％).These articles mainly included basic science,inventory survey,diagnostic methods and clinical treatment strategy,and the most published form was treatise (77 articles,90.6％).The author who published the most articles was YUAN Gui yong from Nanjing (3 articles,3.5％),the institution which published the most articles was People's Hospital of Hainan Province (4 articles,4.7％),and the provinces or cities which published the most articles were Beijing (15 articles,17.6％),Shandong (9 articles,10.6％) and Zhejiang (7 articles,8.2％) by turns.Among the 85 articles,31 articles (36.5％) were supported by foundations.The definition,epidemiology,mechanism,influence factors,clinical characteristics,diagnosis and treatment strategy of elderly depression,and especially secondary depression were well discussed in these literatures.Conclusions The literatures of elderly depression published in Chinese Medical Association journals are insufficient in quantity,and more than half of the literatures are published from 2010 to 2012.The distribution of journals is relatively concentrated,but the distribution of author,institution and region in these articles is dispersed.Secondary depression in the elderly is concerned especially.

Objective To evaluate the effects of compositive cognitive training on reasoning ability for healthy community elderly.Method All the participants were selected from one district in Shanghai by every 50 samples.151 community healthy elderly who accorded with the standard were collected at last.They were divided into cognitive intervention group ( n =90) and control group ( n =61 ) by sequence.The interventions ( includes reasoning,memory training,et al) were conducted in 24 sessions over 12 weeks.All individuals were assessed by Neuropsychological Test Battery for Elderly (NTBE) at baseline,follow-up,half a year follow-up and one year follow-up phase,and Raven' s Standard Progressive Matrices on reasoning classes.Results The scores of Raven' s Standard Progressive Matrices were significantly higher after reasoning training in intervention group( (15.54 ±5.70),(10.35 ± 5.10),t =3.595,P ＜ 0.01 ).Compared with baseline,the reasoning test scores of NTBE were significantly higher after cognitive intervention in treatment group at follow-up phase,and were also significantly higher than control group (P ＜ 0.05 or P ＜ 0.01 ).Conclusion Results support the effectiveness of compositive cognitive training in improving reasoning ability are positive,and can last for one year.

Objective To compare the prevalence and genotype distribution of human papillomavirus (HPV) between human immunodeficiency virus (HIV)-positive and-negative populations.Methods Patients with condyloma acuminatum or persons who recently had sexual contact with patients with condyloma acuminatum were enrolled into this study,and classified into HIV-positive group (n =62) and HIV-negative group (n =2 716).GeneChip analysis was performed to detect HPV and determine HPV genotypes in mucocutaneous samples collected from the external genitalia of these subjects.Statistical analysis was carried out by chi-square test using the SPSS software version 19.0.Results The prevalence rates of HPV infection,high-risk HPV types and low-risk HPV types were significantly higher in HIV-positive persons than in HIV-negative persons (74.19％ (46/62) vs.42.30％ (1 149/2 716),67.74％ (42/62) vs.29.57％ (803/2 716),58.06％ (36/62) vs.24.71％ (671/2 716),respectively,all P＜ 0.01).The detection rate of HPV was also increased in HIV-positive men compared with HIV-negative men (92.11％ (35/38) vs.37.38％ (382/1 022),x2 =45.98,P ＜ 0.01).Although the top three genotypes of low-risk HPV were types 6,43 and 11 in both HIV-positive and-negative groups,the prevalence rate of HPV 6 was 37.10％ (23/62) in HIV-positive group,but only 11.12％ (302/2 716) in HIV-negative group.The top five genotypes of high-risk HPV were types 16 (22.58％,14/62),52,66,58 and 18 in HIV-positive group,and types 16 (7.77％,211/2716),58,56,66 and 52 in HIV-negative group.Coinfections with multiple HPV subtypes were common in both groups,and the number of concurrent HPV genotypes was as high as 8 in HIV-positive group,and 9 in HIV-negative group.The prevalence rate of coinfections with three or more HPV genotypes in HIV-positive group was significantly higher than that in HIV-negative group (65.21％ (30/46) vs.16.71％ (192/1 149)).Conclusions Compared with HIV-negative populations,HIV-positive populations show elevated prevalence of HPV infection,high-risk HPV genotypes and low-risk HPV genotypes.Moreover,the prevalence of HPV is higher in HIV-positive men than in HIV-negative men.These findings are worthy of further attention in clinic.

Objective To investigate the safety of autologous blood component transfusion during cesarean section in patients with Rh (D)-negative blood group.Methods Thirty ASA Ⅰ or Ⅱ patients of Rh (D)-negative blood group, aged 20-35 yr, weighing 50-80 kg, undergoing elective cesarean section, were enrolled in this study.After lactated Ringer' s solution 7 ml/kg was infused, blood was obtained from radial artery at a rate of 60-80ml/min, and blood volume was maintained by simultaneous infusion of 6% hydroxyethyl starch 130/0.4 at the same rate. The collected blood was subjected to two cycles of autologous blood component separation. Blood collecting during each cycle was stopped 15 s after red blood cells were separated. The autologous blood was infused when the blood loss≥20% of blood volume. The autologous blood was infused after suture of the uterus when the blood loss < 20% of blood volume. The parameters of maternal vital signs and fetal heart rate were monitored. Hypotension and tachycardia were recorded during autologous blood collecting. SpO2 was monitored routinely. Venous blood samples were taken before blood collecting (baseline), at the end of blood collecting, before autologous blood transfusion, 24 h after operation for determination of Hb, Hct, Plt, PT, APTT, INR and Fib. Umbilical arterial blood samples were obtained after delivery for blood gas analysis. Apgar score was recorded at 1 and 5 min after birth. Blood loss and allogeneic blood transfusion were also recorded. Results No hypotension and tachycardia occurred during the process of blood collecting and the fetal heart rate was within the normal range. Compared with the baseline value, there were no significant differences in SpO2 , Hb, Hct, Plt, PT, APTT, INR and FIB value at the other time points. The pH value and concentrations of base excess and lactate were within the normal range.The Apgar score was (9.0 ±0.8) and (9.2 ± 0.8) at 1 and 5 min after birth respectively. The blood loss during operation was (405 ± 28) ml and no patients received homologous blood transfusion. Conclusion The safety of autologous blood component transfusion is good during cesarean section in Rh (D)-negative blood group patients.