Objective To explore the work environment of male nurses of psychiatric in Chongqing, as well as the affecting factors. Methods The Professional Practice Environment (PPE) scale was applied to investigate the work environment of 400 male nurses of psychiatric in Chongqing. And the regression analysis was used to analyze the associated factors. Results staff relationships with physicianswas the subscale with the highest score (3.15 ± 0.41) of the PPE of the tested male nurses of psychiatric in Chongqing, whileteamworkwas the subscale with the lowest score (2.05±0.70). Locations and types of hospitals,nurse-patient ratios and education of male nurses were identified to be predictors of work environment (t=- 14.19- 7.63, P<0.01). Conclusions Multiple factors affecting the work environment of male nurses of psychiatric should be considered when nursing managers draw up management strategies.