1.Rhinocerebral mucormycosis: report of two cases.
Chun-yan HE ; Yu-lan JIN ; Hong-gang LIU
Chinese Journal of Pathology 2010;39(5):345-346
Adolescent
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Aged
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Aspergillosis
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microbiology
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pathology
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Aspergillus
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isolation & purification
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Brain Diseases
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drug therapy
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microbiology
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pathology
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surgery
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Diabetes Complications
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microbiology
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Diagnosis, Differential
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Female
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Humans
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Male
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Mucorales
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isolation & purification
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Mucormycosis
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drug therapy
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pathology
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surgery
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Nose Diseases
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drug therapy
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microbiology
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pathology
;
surgery
2.Modulatory effect of curcumin on the adhesion and migration of Raji cells
Qiuling WU ; Yan CHEN ; Chun ZHANG ; Jing HE
Journal of Chinese Physician 2000;0(12):-
Objective To study the effect of SDF1/CXCR4 and FN/?1 integrin modulated by curcumin on the adhesion and migration of B lymphoma Raji cell and to explore the mechanism of curcumin to inhibit the invasiveness of lymphoma.Methods The experiment consisted of eight groups: Control,FN,SDF,FN+anti-?1 integrin,SDF+anti-CXCR4,curcumin+FN,curcumin+SDF.Adhesion assay and migration assay were used to detect the difference of invasiveness among different groups.Results In vitro,SDF1 and FN can increase the adhesion and migration of Raji cells,which can be blocked by ?1 integrin antibody and CXCR4 antibody.Curcumin can inhibit the adhesion and migration of Raji to FN in a dose-dependant manner.Low dosage curcumin had minimal effect on the adhesion and migration of Raji induced by SDF except that 25?mol/L curcumin can inhibit the adhesion and migration of Raji.Conclusion FN/?1 integrin and SDF1/CXCR4 pathway play important roles in the adhesion and migration of Raji cells.Curcumin can decrease the invasiveness of Raji cells through inhibiting FN/?1 integrin.It has good prospect of clinical application.
3.Effects of the Culture Method on the Construction of Dermal Substitutes in vitro
Yan-Na HE ; Chun-Mei DING ; Hua JIANG ; Yan ZHOU ; Wen-Song TAN ;
China Biotechnology 2006;0(05):-
Culture environment is the key factor in the construction of dermal skin.It was investigated that the effects of the culture methods,including the static culture and spinner flask culture,and stir speeds on the cells proliferation,metabolism and distribution within collagenchitosan sponges.A higher cell density and specific growth rate was obtained with spinner flask culture versus static culture,especially,the 80 r/min spinner flask culture.The cell distribution in dermal substitutes from stirred culture system was more uniform than static culture,as well as that with increase of stir speeds in spinner flask.In summary,the spinner flasks culture with proper stir speed shows promise for the construction of dermal substitutes in vitro.
4.Identification and application of three anti-HCMVpp65 McAbs
Chun-Yan QIAN ; Sheng-Nian WANG ; Yue ZHANG ; Ying-Ying CHEN ; Yan SHI ; Yan HE ; Hao LU ; Yu-Hua JI ;
Chinese Journal of Laboratory Medicine 2001;0(02):-
0.05).Conclusion Three Hybridoma cell lines which secrete the target antibodies with satisfied affinities and specificities have been successfully raised,which provides a basis to produce a domestic-made HCMVpp65 antigen diagnosis kit.
5.Progress in the biomarker discovery for drug-induced liver injury.
Lei-yan HE ; Yao-xue GUO ; Chun LI ; Ye DENG ; Qi-zhi ZHANG ; Wen-xing PENG
Acta Pharmaceutica Sinica 2015;50(8):959-965
The leading cause of drug withdrawal from market and clinical trials failure is drug-induced liver injury (DILI). Varying clinical, histological and laboratory features of DILI, as well as undefined underlying mechanisms, hinder patients to be diagnosed in the early-stage of the disease and receive effective treatments. Conventional indicators, like serum transaminases and bilirubin, have inevitable limitations referring to sensitive prediction and specific detection of DILI. In order to reduce the occurrence of DILI, researchers have attempted to discover potential biomarkers with higher specificity and sensitivity from blood and urine in recent years. This article aims to review recent advances in biomarkers of DILI.
Biomarkers
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blood
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urine
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Chemical and Drug Induced Liver Injury
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diagnosis
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Humans
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Sensitivity and Specificity
6.The effect of brain-derived neurotrophic factor on the angiogenesis.
Chun-yan SUN ; Yu HU ; Tao WU ; Ya-dan WANG ; Hua-fang WANG ; Wen-juan HE
Chinese Journal of Pathology 2006;35(4):238-239
Animals
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Brain-Derived Neurotrophic Factor
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pharmacology
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Cell Movement
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drug effects
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Cell Proliferation
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drug effects
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Cells, Cultured
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Chick Embryo
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Chorioallantoic Membrane
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blood supply
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Endothelial Cells
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cytology
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drug effects
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physiology
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Female
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Humans
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Mice
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Mice, Inbred C57BL
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Neovascularization, Physiologic
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drug effects
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Vascular Endothelial Growth Factor A
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pharmacology
7.Evaluation of the rapid trehalose test for the identification of the Candida glabrata
Chun HE ; Qin LIN ; Zhenying BAO ; Yanhong MENG ; Xiaomei QI ; Hong HUA ; Zhimin YAN
Journal of Peking University(Health Sciences) 2015;(1):186-190
Objective:To explore a rapid and cost-effective method for identification of Candida gla-brata through the comparison of two different methods , using molecular methods of sequencing as gold standard.Methods:From our clinic, 200 strains of suspected Candida glabrata were collected during the last 3 years and selected after incubation in CHROMagar Candida medium for 48 h.By comparing the results of the CHROMagar Candida medium, the identification of the rapid trehalose test for different kinds of strains were analyzed under incubation in the tubes for 3 h, 6 h, and 24 h at 37 ℃and 42 ℃, respectively .All the strains were identified to species level by methods of sequencing .The optimal time and temperature of the trehalose test for the identification of Candida glabrata were assessed .Two different methods, CHROMagar Candida medium and the rapid trehalose test , in identification of Candi-da glabrata were compared.Results:In all the 200 strains, Candida glabrata ferment trehalose with 3 h incubation under 42 ℃ were the optimal time and temperature for fermenting trehalose .The accuracy , sensitivity, and specificity of the rapid trehalose test were 99.00% (198/200), 98.66% (147/149) and 100.00%(51/51).The accuracy rate of CHROMagar Candida medium was 79.50%(159/200), the sensitivity and specificity were only 89.93%(134/149) and 49.02%(25/51), however, compared with the domestic current popular methods , the rapid trehalose test had better time efficiency ratio .Con-clusion:The evaluation results suggest that the rapid trehalose test has advantages in terms of operational convenience and low cost , and the results can be obtained in 3 h.Therefore, it has application value in clinical laboratory .
8.Equipment and technology requirements of medical emergency response to nuclear and radiation accident
Qisheng JIANG ; Fengsheng LI ; Wei LI ; Rui HE ; Chun YAN ; Shu CHEN
Chinese Medical Equipment Journal 1993;0(05):-
Medical emergency response is a part of emergency response to nuclear accident,and the equipment and technology play an important role in the process.So it's important and necessary to research the equipment and technology requirements of medical emergency response to nuclear and radiation accident.The aim of the paper is to screen and list some basic equipments and technology in medical emergency response to nuclear and radiation accident,such as individual internal and external exposure dose monitor,radiation detection appliance,decontamination and radiation protection equipment as well as first-aid instrument.
9.A prospective randomized controlled study on topical autologous platelet-rich plasma gel for the treatment of diabetic non-healing dermal ulcers
Yan WANG ; Chun WANG ; Liping HE ; Yanzhi YANG ; Lifang LU ; Guanjian LIU ; Xingwu RAN
Chinese Journal of Diabetes 2009;17(11):822-825,836
Objective To compare the safety and effectiveness between treatments with autologous platelet gel (APG) versus standard care for treating refractory diabetic dermal ulcers.Methods The 46 patients with proved nonhealing diabetic dermal ulcers were enrolled. Eligible for the study were patients with grade II/III ulcers according to Wagner, lasting for at least 2 weeks and with no signs of infection at recruitment.Patients were given their informed consent document and randomly assigned to two groups: standard care (ST, n=23) or standard care plus topic application of APG (APG, n=23) for twelve weeks.The treatment of blood glucose, blood pressure and lipids was optimized and the empiric antibiotic treatment was further adjusted according to the results of culture and sensitivity testing in all patients. APG treatment consisted of wound dressing with APG, followed by topical washing and cleaning. The APG was then covered with vaseline gauze for 72 hours, after which the ulcers were treated by standard care. Participants were seen thrice a week, twice a week, or at weekly intervals. Twelve weeks observation was set as the end point.Results The would of APG group were improved in 22 patients with ulcers healed completely and 1 case with would area reduced. In the ST group, 13 ulcers were healed, 6 worsened and 4 with would area reduced. The cumulative rate of ulcer healing was 95.7% in the APG group versus 56.5% in the ST group (P=0.002). The total effective rate in APG vs ST group was 100.0% vs 73.9% (P=0.009). By Kaplan-Meier analysis,the time-to-healing of ulcer and time-to-lutation of sinus were significantly different between two groups (log-rank, P=0.006, 0.000, respectively). No treatment-related adverse events were observed. Conclusions Treatment with APG in addition to standard care results in a significantly faster and better healing for a refractory diabetic dermal ulcer and does not raise any safety concerns. So APG treatment can be a valuable and effective aid in the management of diabetic foot ulcers.
10.Updates on inflammatory myofibroblastic tumor of head and neck region.
Chinese Journal of Pathology 2013;42(10):712-714
Actins
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metabolism
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Diagnosis, Differential
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Granuloma, Plasma Cell
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diagnostic imaging
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drug therapy
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metabolism
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pathology
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surgery
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Head and Neck Neoplasms
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diagnostic imaging
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drug therapy
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metabolism
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pathology
;
surgery
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Humans
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Neoplasm Recurrence, Local
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Protein Kinase Inhibitors
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therapeutic use
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Pyrazoles
;
therapeutic use
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Pyridines
;
therapeutic use
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Tomography, X-Ray Computed
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Vimentin
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metabolism