1.Rhinocerebral mucormycosis: report of two cases.
Chun-yan HE ; Yu-lan JIN ; Hong-gang LIU
Chinese Journal of Pathology 2010;39(5):345-346
Adolescent
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Aged
;
Aspergillosis
;
microbiology
;
pathology
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Aspergillus
;
isolation & purification
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Brain Diseases
;
drug therapy
;
microbiology
;
pathology
;
surgery
;
Diabetes Complications
;
microbiology
;
Diagnosis, Differential
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Female
;
Humans
;
Male
;
Mucorales
;
isolation & purification
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Mucormycosis
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drug therapy
;
pathology
;
surgery
;
Nose Diseases
;
drug therapy
;
microbiology
;
pathology
;
surgery
2.Modulatory effect of curcumin on the adhesion and migration of Raji cells
Qiuling WU ; Yan CHEN ; Chun ZHANG ; Jing HE
Journal of Chinese Physician 2000;0(12):-
Objective To study the effect of SDF1/CXCR4 and FN/?1 integrin modulated by curcumin on the adhesion and migration of B lymphoma Raji cell and to explore the mechanism of curcumin to inhibit the invasiveness of lymphoma.Methods The experiment consisted of eight groups: Control,FN,SDF,FN+anti-?1 integrin,SDF+anti-CXCR4,curcumin+FN,curcumin+SDF.Adhesion assay and migration assay were used to detect the difference of invasiveness among different groups.Results In vitro,SDF1 and FN can increase the adhesion and migration of Raji cells,which can be blocked by ?1 integrin antibody and CXCR4 antibody.Curcumin can inhibit the adhesion and migration of Raji to FN in a dose-dependant manner.Low dosage curcumin had minimal effect on the adhesion and migration of Raji induced by SDF except that 25?mol/L curcumin can inhibit the adhesion and migration of Raji.Conclusion FN/?1 integrin and SDF1/CXCR4 pathway play important roles in the adhesion and migration of Raji cells.Curcumin can decrease the invasiveness of Raji cells through inhibiting FN/?1 integrin.It has good prospect of clinical application.
3.Effects of the Culture Method on the Construction of Dermal Substitutes in vitro
Yan-Na HE ; Chun-Mei DING ; Hua JIANG ; Yan ZHOU ; Wen-Song TAN ;
China Biotechnology 2006;0(05):-
Culture environment is the key factor in the construction of dermal skin.It was investigated that the effects of the culture methods,including the static culture and spinner flask culture,and stir speeds on the cells proliferation,metabolism and distribution within collagenchitosan sponges.A higher cell density and specific growth rate was obtained with spinner flask culture versus static culture,especially,the 80 r/min spinner flask culture.The cell distribution in dermal substitutes from stirred culture system was more uniform than static culture,as well as that with increase of stir speeds in spinner flask.In summary,the spinner flasks culture with proper stir speed shows promise for the construction of dermal substitutes in vitro.
4.Identification and application of three anti-HCMVpp65 McAbs
Chun-Yan QIAN ; Sheng-Nian WANG ; Yue ZHANG ; Ying-Ying CHEN ; Yan SHI ; Yan HE ; Hao LU ; Yu-Hua JI ;
Chinese Journal of Laboratory Medicine 2001;0(02):-
0.05).Conclusion Three Hybridoma cell lines which secrete the target antibodies with satisfied affinities and specificities have been successfully raised,which provides a basis to produce a domestic-made HCMVpp65 antigen diagnosis kit.
5.Recurrent malignant epithelioid angiomyolipoma of kidney: report of a case.
Jiang DU ; Yu-lan JIN ; Chun-yan HE ; Ming LI ; Hong-gang LIU
Chinese Journal of Pathology 2010;39(4):275-276
Adult
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Angiomyolipoma
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metabolism
;
pathology
;
surgery
;
Carcinoma, Renal Cell
;
metabolism
;
pathology
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Diagnosis, Differential
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Humans
;
Kidney Neoplasms
;
metabolism
;
pathology
;
surgery
;
MART-1 Antigen
;
metabolism
;
Male
;
Melanoma-Specific Antigens
;
metabolism
;
Tumor Suppressor Protein p53
;
metabolism
6.Progress in the biomarker discovery for drug-induced liver injury.
Lei-yan HE ; Yao-xue GUO ; Chun LI ; Ye DENG ; Qi-zhi ZHANG ; Wen-xing PENG
Acta Pharmaceutica Sinica 2015;50(8):959-965
The leading cause of drug withdrawal from market and clinical trials failure is drug-induced liver injury (DILI). Varying clinical, histological and laboratory features of DILI, as well as undefined underlying mechanisms, hinder patients to be diagnosed in the early-stage of the disease and receive effective treatments. Conventional indicators, like serum transaminases and bilirubin, have inevitable limitations referring to sensitive prediction and specific detection of DILI. In order to reduce the occurrence of DILI, researchers have attempted to discover potential biomarkers with higher specificity and sensitivity from blood and urine in recent years. This article aims to review recent advances in biomarkers of DILI.
Biomarkers
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blood
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urine
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Chemical and Drug Induced Liver Injury
;
diagnosis
;
Humans
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Sensitivity and Specificity
7.The effect of brain-derived neurotrophic factor on the angiogenesis.
Chun-yan SUN ; Yu HU ; Tao WU ; Ya-dan WANG ; Hua-fang WANG ; Wen-juan HE
Chinese Journal of Pathology 2006;35(4):238-239
Animals
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Brain-Derived Neurotrophic Factor
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pharmacology
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Cell Movement
;
drug effects
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Cell Proliferation
;
drug effects
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Cells, Cultured
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Chick Embryo
;
Chorioallantoic Membrane
;
blood supply
;
Endothelial Cells
;
cytology
;
drug effects
;
physiology
;
Female
;
Humans
;
Mice
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Mice, Inbred C57BL
;
Neovascularization, Physiologic
;
drug effects
;
Vascular Endothelial Growth Factor A
;
pharmacology
8.Effects of warming the yang and benefiting the marrow on the expression of matrix metalloproteinases in articular cartilage of rabbits with knee osteoarthritis
Pengcheng SHAN ; Mingjiang HE ; Hongmei ZHANG ; Lin JING ; Chun ZHANG ; Renzhuo ZHANG ; Qi YAN ; Yihui SHA
Chinese Journal of Tissue Engineering Research 2014;(7):997-1002
BACKGROUND:There are few reports concerning effects of warming the yang and benefiting the marrow for the knee osteoarthritis on the expression of matrix metal oproteinase.
OBJECTIVE:To observe the effect of warming the yang and benefiting the marrow on the expression of matrix metal oproteinase in rabbit models of knee osteoarthritis.
METHODS:Of 96 healthy adult New Zealand rabbits, 72 rabbits were randomly selected for making rabbit models of knee osteoarthritis using plaster external fixation. After success model establishment, the rabbits were randomly assigned to three groups. Model group was left intact. Chinese medicine group received daily intragastric administration of drug extract 24 mL/kg. Drug control group was daily intragastrical y administered Puli Capsule (glucosamine hydrochloride) 24 mg/kg, once a day, until the eighth week of success model induction. An additional 24 New Zealand rabbits served as blank controls.
RESULTS AND CONCLUSION:Using quantitative PCR, matrix metal oproteinase-1, matrix metal oproteinase-3 and matrix metal oproteinase-13 expression was significantly higher in the model group than that in the other three groups. Matrix metal oproteinase-1, matrix metal oproteinase-3 and matrix metal oproteinase-13 expression was significantly lower in the Chinese medicine group and drug control group than that in the model group. These results indicated that warming the yang and benefiting the marrow for knee osteoarthritis in rabbits could effectively inhibit the expression of matrix metal oproteinase in rabbits.
9.Study on dynamic growth of Codonopsis pilosula.
Chun-Yu HE ; Yan-Hong ZHANG ; Hai-Ming LIN
China Journal of Chinese Materia Medica 2006;31(4):285-289
OBJECTIVETo study on the dynamic changes of Codnopsis pilosula for the guidance of the field management.
METHODUsing the random method the influences of the cultivating density and fertilizing weight at four different levels was observed.
RESULT AND CONCLUSIONThe dynamic changes of the biomass, growth period andbiological changes at different growth stages were found out. The most predominant combination of the two factors, the 1.05 million roots/hm2 of the cultivating density and 240 kg x hm(-2) of the fertilizing weight reached the highest accumulation of stems & leaves; 652 mg x d(-1) the 0.6 million roots/hm2 and 240 kg x hm(-2) reached the heaviest single fresh root. The results could be used for the cultivating, fertilizing and practicing SOP of C. pilosula.
Biomass ; Codonopsis ; anatomy & histology ; growth & development ; Fertilizers ; Plant Leaves ; anatomy & histology ; Plant Roots ; anatomy & histology ; Plant Stems ; anatomy & histology ; Plants, Medicinal ; anatomy & histology ; growth & development ; Seasons
10.Updates on inflammatory myofibroblastic tumor of head and neck region.
Chinese Journal of Pathology 2013;42(10):712-714
Actins
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metabolism
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Diagnosis, Differential
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Granuloma, Plasma Cell
;
diagnostic imaging
;
drug therapy
;
metabolism
;
pathology
;
surgery
;
Head and Neck Neoplasms
;
diagnostic imaging
;
drug therapy
;
metabolism
;
pathology
;
surgery
;
Humans
;
Neoplasm Recurrence, Local
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Protein Kinase Inhibitors
;
therapeutic use
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Pyrazoles
;
therapeutic use
;
Pyridines
;
therapeutic use
;
Tomography, X-Ray Computed
;
Vimentin
;
metabolism