1.Primary chondroma of ovary: report of a case.
Xiao-mei LIU ; Yu-xin WANG ; Chun-bo NIU
Chinese Journal of Pathology 2011;40(12):845-846
2.Clinical evaluation of Nickel-Titanium rotary instruments in preparation of root canals
Chun-Mei YU ; Yi-Yun FANG ; Xue-Xun WU ;
Chinese Journal of Primary Medicine and Pharmacy 2006;0(12):-
Objective To study and assess clinical application of two Nickel-Titanium(NiTi)rotary instru- ments,namely ProTaper and Hero 642,in preparation of root canals.Methods 125 teeth were divided into three groups and respectively instrumented by stainless K-files,ProTaper or Hero 642 rotary instruments.All teeth were obturated with lateral condensation method.The efficiency of preparation and obturation was analyzed with radio- graphs.Results NiTi rotary instruments were better in keeping the curvature and flow of curved canals than stain- less K files.There was no transportation,apical blockage and ledge in NiTi groups.The operative time was shorter and endodontic flare-up seldom occurred in NiTi groups.Conclusion The NiTi rotary instrumentation technique could be used to prepare curved root canals effectively and quickly.The future use of NiTi engine-driven rotary in- strument appeared to be promising.
3.Case of sublingual cavernous hemangioma.
Ying-Chun TENG ; Dong-Dong YU ; Mei LU
Chinese Acupuncture & Moxibustion 2014;34(1):40-40
4.Perivascular epithelioid cell tumor (PEComa) of the uterine.
Mei-fu GAN ; Mei JIN ; Chun-kai YU ; Ju-fang CAI
Chinese Journal of Pathology 2006;35(5):314-315
Actins
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metabolism
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Adult
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Antigens, Neoplasm
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metabolism
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Diagnosis, Differential
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Epithelioid Cells
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chemistry
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pathology
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Female
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Humans
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Hysterectomy
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Immunohistochemistry
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Melanoma-Specific Antigens
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Mesenchymoma
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metabolism
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pathology
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surgery
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Neoplasm Proteins
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metabolism
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Uterine Neoplasms
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metabolism
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pathology
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surgery
5.Effects of acid and alkali stress on ginsenoside content and histochemical localization of ginsenoside in adventitious root of Panax ginseng.
Tie-Jun LI ; Mei-Lan LIAN ; Chun-Hui SHAO ; Dan YU ; Xuan-Chun PIAO
China Journal of Chinese Materia Medica 2013;38(24):4277-4280
To investigate the effect of acid and alkali stress on ginsenoside content of Panax ginseng, adventitious roots culture in bioreactors were incubated for 30 d and pH value was adjusted. Ginsenoside content increased by reducing or raising the pH in culture medium, the muxium ginsenoside content was determined on the 5th days after acid treatment and on the 7th days after alkali treatment. The result of histochemical localization of ginsenoside revealed that the red color from light to dark were found in the adventitious root tissue, and ginsenoside mainly located in the pericycle cells where appeared the dark red color.
Ginsenosides
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metabolism
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Hydrogen-Ion Concentration
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Panax
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metabolism
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physiology
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Plant Roots
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metabolism
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Stress, Physiological
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Time Factors
6.Effects of several factors on cell growth and ginsenoside accumulation of Panax ginseng suspension culture.
Tie-Jun LI ; Mei-Lan LIAN ; Dan YU ; Chun-Hui SHAO ; Xuan-Chun PIAO
China Journal of Chinese Materia Medica 2013;38(23):4047-4051
To improve cell suspension culture system of Panax ginseng, the dynamic of cell growth and medium consumption were studied, and the effects of filter on the culture vessel, revolution number, and inoculation density on cell growth and ginsenoside accumulation were also investigated. The maximum cell growth and ginsenoside accumulation was found on the 20th days of suspension culture, therefore, 20 days were confirmed as a suitable culture period for mass production of ginsenoside. Cell growth and ginsenoside content were promoted when the culture vessel had a ventilated filter. Revolution speed during suspension culture affected cell growth, but not ginsenoside content, a peak of ginsenoside productivity was found in the treatment of 120 r x min(-1). Inoculation density also influenced cell growth and ginsenoside accumulation, inoculation density of 6 g was better than other inoculation densities, the ginsenoside content and productivity were up to 12.8 mg x g(-1) DW and 146.6 mg x L(-1), respectively.
Cell Culture Techniques
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methods
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Cell Proliferation
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Culture Media
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chemistry
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Ginsenosides
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metabolism
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Panax
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cytology
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growth & development
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metabolism
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Suspensions
7.Influence of Naloxone on ?-Exdorphin and Calcitonin Gene-Related Peptide in Convulsive Children with Brain Damage
chun-mei, JIA ; dong-mei, WANG ; ya-nan, XIN ; qiao-lian, WANG ; jian-hui, ZHANG ; yu, WANG
Journal of Applied Clinical Pediatrics 2006;0(20):-
0.01),which were significantly different from group Ⅲ(P
8.The interactions between natural products and OATP1B1.
Mei-zhi SHI ; Yu LIU ; Jia-lin BIAN ; Meng JIN ; Chun-shan GUI
Acta Pharmaceutica Sinica 2015;50(7):848-853
Organic anion transporting polypeptide 1B1 (OATP1B1) is an important liver-specific uptake transporter, which mediates transport of numerous endogenous substances and drugs from blood into hepatocytes. To identify and investigate potential modulators of OATP1B1 from natural products, the effect of 21 frequently used natural compounds and extracts on OATP1B1-mediated fluorescein methotrexate transport was studied by using Chinese hamster ovary cells stably expressing OATP1B1 (CHO-OATP1B1) in 96-well plates. This method could be used for the screening of large compound libraries. Our studies showed that some flavonoids (e.g., quercetin, quercitrin, rutin, chrysanthemum flavonoids and mulberrin) and triterpenoids (e.g., glycyrrhetinic acid and glycyrrhizic acid) were inhibitors of OATP1B1 with IC50 values less than 16 µmol · L(-1). The IC50 value of glycyrrhetinic acid on OATP1B1 was comparable to its blood concentration in clinics, indicating an OATPlB1-mediated drug-drug interaction could occur. Structure-activity relationship analysis showed that flavonoids had much higher inhibitory activity than their glycosides. Furthermore, the type and length of saccharides had a significant effect on their activity. In addition, we used OATP1B1 substrates fluvastatin and rosuvastatin as probe drugs to investigate the substrate-dependent effect of several natural compounds on the function of OATP1B1 in vitro. Our results demonstrated that the effect of these natural products on the function of OATPlB1 was substrate-dependent. In summary, this study would be conducive to predicting and avoiding potential OATP1B1-mediated drug-drug and drug-food interactions and thus provide the experimental basis and guidance for rational drug use.
Animals
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Biological Products
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CHO Cells
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Cricetulus
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Drug Interactions
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Fatty Acids, Monounsaturated
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pharmacology
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Flavonoids
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pharmacology
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Indoles
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pharmacology
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Inhibitory Concentration 50
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Organic Anion Transporters
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genetics
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metabolism
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Rosuvastatin Calcium
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pharmacology
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Structure-Activity Relationship
9.Studyies on the Breeding and Cultivation of L-Lactic Acid Producing Strain
Chun-Mei GE ; Shao-Bin GU ; Jian-Ming YAO ; Ren-Rui PAN ; Zeng-Liang YU ;
Microbiology 1992;0(05):-
In order to obtain higher L-lactic acid yield industrial strain, the original strain Rhizopus oryzae PW352 was mutated by means of N+ ions implantation and a mutant strain Rhizopus oryzae RE3303 was obtained. Its lactic acid yield was increased by 75% than that of the original one. The acid producing condition was optimized by orthogonal design. The concentration of L-lactic acid reached to 131~136g/L and the conversion rate of glucose was as high as 86%~90% under the optimum condition.
10.Construction of TK Gene-deleted PRV SH StrainContaining a Single LoxP Site
Min-Xiu WANG ; Xin-Ming SU ; Chun-Mei YU ; Rui-Bing CAO ; Pu-Yan CHEN ;
China Biotechnology 2006;0(10):-
Pseudorabies virus (PRV) is a swine herpesvirus of the Alphaherpesvirinae subfamily and a pathogen of swine resulting in devastating disease and economic losses worldwide. Cre/loxP site-specific system has the character of site specific, time specific, tissue specific and high efficiency in recombination, which makes this system universal in vivo and in vitro recombination of bacteria, fungus, plants, insects and mammals. A recombinant PRV which contain a loxP site in TK locus by using Cre/LoxP recombinant system was construsted. A pair of primers were synthesized according to the pEGFP-C1 sequence published on GenBank, and were used to amplify the EGFP gene expression cassette with two loxP sites flanking each side. This target gene was cloned into pSKLR, the resulting transfer vector pSKLR-GFP-loxP was then cotransfected into 293T cells with PRV SH strain genomic DNA. The recombinant virus rPRV1 was selected and purified in TK-143 cells by choosing fluorescent expressing plaques. Cre expression vector pOG231 was cotransfected into 293T cells with rPRV1 genomic DNA. The second recombinant virus rPRV2 was obtained, which contains only one loxP site in TK locus. Sequencing results of rPRV2 TK gene indicated that 34bp loxP site was inserted into rPRV2 genome and there were 270bp deletion in TK gene. PCR amplifying different generations of rPRV2 TK gene showed that the mutant was stable when passages in RK-13 cells. TCID_ 50 assay indicated that rPRV2 grows well on RK-13 cells. The LD_ 50 test results on BALB/C mice suggested that the virulence of rPRV2 was reduced. As a conclusion, the report gene GFP expression cassette was removed successfully from rPRV1 genome and only one LoxP site was leaved in rPRV2 genome by using Cre/LoxP recombinant system.