·AIM: To report two patients with Helveston syndrome who received surgical treatment.·METHODS: During last year in department of oph-thalmology in West China Hospital, the surgery was designed to correct A-exotropia by lateral rectus' weaken and vertical offsets, and dissociated vertical deviation (DVD) by superior rectus muscles recession simultaneously.·RESULTS: A-exotropia and DVD of the patients vanished six months after the surgery. Both patients were clinically cured.·CONCLUSION: As the degree of horizontal and vertical strabismus was in the allowed range, complete examination and full assessment of the function of the four muscles were performed before operation, single operation can be considered in the treatment of Helveston syndrome.

Objective To investigate the efficacy and safety of polyethersulfone highlux dialyzer for maintenance hemodialysis patients. Methods Thirty-six maintenance hemodialysis patients were randomizedresigned into two groups,polyethersulfone 14HF(PES-14HF)group and polyethersulfone 150DS(PES-150DS)group,based on a random number table. The patients from two groups received different dialyzer hemodialysis for over 3.5 hours/each time respectively. Changes of serum creatinine,urea,β2-microglobulin,hemoglobin and albumin levels were measured for determination of the efficacy and safety evaluation. Results In the comparison between before or after hemodialysis,the level of serum creatinine,urea,β2-microglobulin levels decreased significantly to(333.8 ± 89. 5)μmol/L,(7. 0 ± 1.9)mmol/L,(22. 9 ± 1.7)mmol/L from(990. 2 ± 191.2)μmol/L,(24. 7 ± 4. 1)mmol/L,(13.6 ± 3.3)mmol/L respectively in the PES1 4 HF group(P ＜ 0.01);the level of serum creatinine,urea,β2-microglobulin levels decreased significantly to(395.5 ± 86.1)μmol/L,(8. 1± 2. 8)mnol/L,(18.0 ± 3.0)mmol/L from(1059. 5 ± 179. 4)μmol/L,(25.3 ± 4. 8)mmol/L,(22. 3 ± 2. 9)mmol/L respectively in the PES-150DS group(P ＜ 0. 01). We found no significant differences in each measured index between two types of hemodialysis(P ＞ 0. 05 respectively),while the level of β2 -microglobulin levels decreased more significantly in the PES14HF group(42. 81 ± 12. 48)mg/L than PES-150DS group(24. 21 ± 13. 24)mg/L(P =0. 017). Conclusions The efficacy and safety of the PES-14HF hollow fiber membrane hemodialyzer is equivalent to that of the PES-150DS hemodialyzer in hemodialysis for uremic patients.

Cell Line, Tumor ; Chromosome Aberrations ; Comparative Genomic Hybridization ; methods ; Gene Amplification ; Gene Dosage ; Gene Expression ; Humans ; Receptor, Fibroblast Growth Factor, Type 1 ; genetics ; metabolism ; Rhabdomyosarcoma ; genetics ; metabolism ; pathology ; Rhabdomyosarcoma, Alveolar ; genetics ; metabolism ; pathology ; Rhabdomyosarcoma, Embryonal ; genetics ; metabolism ; pathology

Objective To explore the plasma ghrelin levels in children and adolescents with short stature and the role of ghrelin in growth hormone-releasing hormone-growth hormone(GHRH-GH) axis.Methods One hundred and fifty-seven children(115 male,42 female) with short stature were selected.Fasting plasma sample was extracted from 10 mL vemous blood of the children with short stature.Insulin tolerance test and arginine stimulation test was performed initially to differential diagnosis.And blood samples was divided into 3 ca-tegories:37 cases of complete growth hormone deficiency (CGHD),52 cases of partial growth hormone deficiency(PGHD) and 68 cases of idiopathic short stature(ISS) during these two growth hormone(GH)provocative tests.Controls consisted of age and gender-match 20 health children.Plasma ghrelin levels were measured by radioimmunoassay.Serum GH was detected by chemiluminescence method,and serum insulin-like growth factor-1 (IGF-1) was measured by using enzyme linked immunosorbent assay.Fasting glucose,insulin,testosterone,estra-diol,luteinizing hormone,follicle-stimulating hormone were measured.Statistical analysis were conducted by using SPSS 11.5 software.Results The fasting ghrelin levels of CGHD group were significantly lower than that of ISS group and control group(Pa0.05).The ghrelin levels were positive correlated with the stimulated GH peak(r=0.176 P0.05).Conclusion Ghrelin has an important role on GH secretion and abnormal secretion of ghrelin might be a reason of growth hormone deficiency which due to hypothalamic abnormality.

Objective:To explore the way and feasibility of carrying out developmental-behavioral pediatrics(DBP) teaching for medical students on probation.Method :To divide 230 medical students on probation into experimental group(120 students) and control group(110 students).The former group spent four class hours in child developmental-behavioral outpatient department and another four hours in sensory integrative therapy room to assist doctors in their training work as volunteers,and their rest probation contents were the same as those of the latter group which had classes according to teaching outline of pediatrics.Test scores of pediatrics of the two groups were compared.Questionnaires filled in by students of experimental group were analyzed.Results of the two groups,scores of pediatrics were not different,which showed that scores weren't influenced by extra teaching contents.Results of questionnaires showed that teaching of DBP was welcome to students,and they had made advances in relative knowledge and their overall quality.Conclusion: It is necessary to carry out DBP teaching.The teaching way of this study has got good effect and is feasible.

Objective To study the similarity of chemical fingerprints of different proportion of Gualoupi-Gualouzi and Fructus Trichosanthis, so as to provide the theoretical basis for the reasonable proportion of Gualoupi-Gualouzi. Methods The HPLC fingerprints and the common patterns of 6 the proportion of the 6 kinds of compatibility (30∶70, 35∶65, 40∶60, 45∶55, 50∶50 and 55∶ 45) of Gualoupi-Gualouzi and Fructus Trichosanthis were established, and the similarity of chemical fingerprints of different compatibility proportion of Gualoupi-Gualouzi and Fructus Trichosanthis between their common patterns were compared. Results Compared with the common pattern of Fructus Trichosanthis, the similarities of the common patterns of chemical fingerprints of the above 6 kinds of compatibility proportion of Gualoupi-Gualouzi were 0.8808±0.0407, 0.8930±0.0236, 0.8995±0.0195, 0.9033±0.0190, 0.9363± 0.0082 and 0.8904±0.0144, respectively. Among them, Gualoupi-Gualouzi ratio of 50∶50 had the highest similarity, and compared with other groups, the differences were statistically significant (P<0.05 or 0.01). Conclusion The optimum proportion of Gualoupi- Gualouzi can be determined by the fingerprint technology, which provides the theoretical basis for the reasonable proportion of Gualou⁃pi-Gualouzi.

Objective To verify the protective effects of Gualou Xiebai dropping pills(GX) on myocardial ischemia-reperfusion injury(MIR.I) in rats. Methods After screening the qualified SPF rats were randomly divided into four groups, with 12 in each group. Sham-operated group and model group rats were respectively treated with normal saline, and rats in GX group and compound Danshen dropping pills group were given the corresponding dropping pills equivalent to 22.5 g/kg and 85.05 mg/kg of the crude herb, respectively. All groups were administered once a day for 7 successive days. One hour after the last administration, the MIRI models were produced by occluding the left coronary artery for 30 min and releasing the occlusion for 120 min. The changes in ST segment were observed, and the contents of creatine kinase-MB (CK-MB), cardiac troponin-T (CTNT) and myoglobin (MYO) in blood plasma were measured. The pathological changes in myocardial tissues were observed by optics and electric-microscope and the percentage of myocardial infarction was measured by detecting the content of Evan blue in myocardium. Results Compared to normal saline in the model group, GX had antagonism to ECG S-T segments elevation in rats with myocardial ischemia, while the contents of CK-MB, MYO, and CTNT in blood plasma declined significantly (P<0.05 or P<0.01); the disorder condition of myocardial cell fiber arrangement was improved, and edema between myocardial tissue and cells was relieved; the inflammatory cell infiltration and myocardial necrosis in cardiac allograft were significantly alleviated, and the percentage of myocardial infarction was decreased significantly (P< 0.01). Conclusion GX may play an important protective role against the MIRI in rats.

Objective: To explore whether the basic principle of Fisher component analysis(FCA) can be used in the category analysis of Fructus Trichosanthis fruit and its processed products. Methods: Their fingerprints were established by using HPLC-PDAD, and the standard fingerprints of them were obtained by digitizing with quercetin as the internal reference peak. Then, their chemical fingerprint information was extracted by FCA and classified by quality model, and the classification results were compared with those classified by principal component analysis and standard atlas analysis. Results: Fructus Trichosanthis and its processed products can be accurately divided into two categories by FCA. Conclusion: FCA can extract the implied chemical information in fingerprints, and analyze them accurately.

Objective To study the in situ intestinal absorption behaviors of 3, 29- dibenzoyl- karounitriol (DK) in Gualou- Xiebai (GX) extract in rats. Methods A rat in situ single-pass perfusion model was used and the concentrations of the perfusate were determined by HPLC-PDAD to investigate the intestinal absorption site and mechanism. Results The main absorption site of DK in GX extract was jejunum, ileum and colon, and the absorption had no significant difference in the three different segments of rat intestine (P>0.05), but was significantly higher than that in duodenum (P<0.05). The Ka and Papp of DK had no significant difference in different concentrations of GX extract (P>0.05). Conclusion DK in GX extract could be absorbed in whole intestinal segment, with the best intestinal absorption site of jejunum, ileum and colon. Its absorbing mechanism may be related to passive diffusion.

Objective To explore whether evolving window orthogonal projection analytical method can be used in the chromatographic peaks matching between traditional Chinese medicine preparation and its medicinal ingredients in prescription. Methods The fingerprints of Gualou Xiebai Baijiu Decoction and the herbs (Gualou and Xiebai) in its prescription were established by using high performance liquid chromatography-photodiode array detector (HPLC-PDAD), and the HPLC peaks in fingerprints of Gualou Xiebai Baijiu Decoction and the herbs in its prescription were matched according to the principle of evolving window orthogonal projection analytical method. Results Evolving window orthogonal projection analytical method could match the chromatogram peaks well between different fingerprints of Gualou Xiebai Baijiu Decoction and the herbs in its prescription. There were 20 common peaks between the ethyl acetate extraction of Gualou Xiebai Baijiu Decoction and Gualou, and 9 common peaks between the n-butanol extraction of Gualou Xiebai Baijiu Decoction and Xiebai. Conclusion Evolving window orthogonal projection analytical method can be used in the chromatographic peaks matching between traditional Chinese medicine preparation and its medicinal ingredients in prescription.