·AIM: To report two patients with Helveston syndrome who received surgical treatment.·METHODS: During last year in department of oph-thalmology in West China Hospital, the surgery was designed to correct A-exotropia by lateral rectus' weaken and vertical offsets, and dissociated vertical deviation (DVD) by superior rectus muscles recession simultaneously.·RESULTS: A-exotropia and DVD of the patients vanished six months after the surgery. Both patients were clinically cured.·CONCLUSION: As the degree of horizontal and vertical strabismus was in the allowed range, complete examination and full assessment of the function of the four muscles were performed before operation, single operation can be considered in the treatment of Helveston syndrome.

Objective To investigate the efficacy and safety of polyethersulfone highlux dialyzer for maintenance hemodialysis patients. Methods Thirty-six maintenance hemodialysis patients were randomizedresigned into two groups,polyethersulfone 14HF(PES-14HF)group and polyethersulfone 150DS(PES-150DS)group,based on a random number table. The patients from two groups received different dialyzer hemodialysis for over 3.5 hours/each time respectively. Changes of serum creatinine,urea,β2-microglobulin,hemoglobin and albumin levels were measured for determination of the efficacy and safety evaluation. Results In the comparison between before or after hemodialysis,the level of serum creatinine,urea,β2-microglobulin levels decreased significantly to(333.8 ± 89. 5)μmol/L,(7. 0 ± 1.9)mmol/L,(22. 9 ± 1.7)mmol/L from(990. 2 ± 191.2)μmol/L,(24. 7 ± 4. 1)mmol/L,(13.6 ± 3.3)mmol/L respectively in the PES1 4 HF group(P ＜ 0.01);the level of serum creatinine,urea,β2-microglobulin levels decreased significantly to(395.5 ± 86.1)μmol/L,(8. 1± 2. 8)mnol/L,(18.0 ± 3.0)mmol/L from(1059. 5 ± 179. 4)μmol/L,(25.3 ± 4. 8)mmol/L,(22. 3 ± 2. 9)mmol/L respectively in the PES-150DS group(P ＜ 0. 01). We found no significant differences in each measured index between two types of hemodialysis(P ＞ 0. 05 respectively),while the level of β2 -microglobulin levels decreased more significantly in the PES14HF group(42. 81 ± 12. 48)mg/L than PES-150DS group(24. 21 ± 13. 24)mg/L(P =0. 017). Conclusions The efficacy and safety of the PES-14HF hollow fiber membrane hemodialyzer is equivalent to that of the PES-150DS hemodialyzer in hemodialysis for uremic patients.

Cell Line, Tumor ; Chromosome Aberrations ; Comparative Genomic Hybridization ; methods ; Gene Amplification ; Gene Dosage ; Gene Expression ; Humans ; Receptor, Fibroblast Growth Factor, Type 1 ; genetics ; metabolism ; Rhabdomyosarcoma ; genetics ; metabolism ; pathology ; Rhabdomyosarcoma, Alveolar ; genetics ; metabolism ; pathology ; Rhabdomyosarcoma, Embryonal ; genetics ; metabolism ; pathology

Objective To explore the plasma ghrelin levels in children and adolescents with short stature and the role of ghrelin in growth hormone-releasing hormone-growth hormone(GHRH-GH) axis.Methods One hundred and fifty-seven children(115 male,42 female) with short stature were selected.Fasting plasma sample was extracted from 10 mL vemous blood of the children with short stature.Insulin tolerance test and arginine stimulation test was performed initially to differential diagnosis.And blood samples was divided into 3 ca-tegories:37 cases of complete growth hormone deficiency (CGHD),52 cases of partial growth hormone deficiency(PGHD) and 68 cases of idiopathic short stature(ISS) during these two growth hormone(GH)provocative tests.Controls consisted of age and gender-match 20 health children.Plasma ghrelin levels were measured by radioimmunoassay.Serum GH was detected by chemiluminescence method,and serum insulin-like growth factor-1 (IGF-1) was measured by using enzyme linked immunosorbent assay.Fasting glucose,insulin,testosterone,estra-diol,luteinizing hormone,follicle-stimulating hormone were measured.Statistical analysis were conducted by using SPSS 11.5 software.Results The fasting ghrelin levels of CGHD group were significantly lower than that of ISS group and control group(Pa0.05).The ghrelin levels were positive correlated with the stimulated GH peak(r=0.176 P0.05).Conclusion Ghrelin has an important role on GH secretion and abnormal secretion of ghrelin might be a reason of growth hormone deficiency which due to hypothalamic abnormality.

OBJECTIVE: To study the chemical fingerprint features and the quantitative analysis of 6 components of Fructus Trichosanthis and its steamed products. METHODS: The chemical fingerprints were established by HPLC with 10 batches of Fructus Trichosanthis and their steamed products, and the contents of 5-hydroxymethyl furfural, vanillic acid, rutin, isoquercitrin, quercetin and luteolin in Fructus Trichosanthis and their steamed products were quantitatively analyzed. The similarity of Fructus Trichosanthis and its steamed products was calculated by the correlation coefficient method and the included angle cosine method, and the contents of 6 components were tested by means of calibration curve. RESULTS: The 10 batch of Fructus Trichosanthis similarity was from 0.82 to 0.86, and the 10 batch of Fructus Trichosanthis steamed products similarity was from 0.97 to 0.98, there was significant difference between the two (P < 0.01). Compared with Fructus Trichosanthis, the contents of 5-hydroxymethyl furfural, vanillic acid, quercetin and luteolin content in steamed products showed significant changes (P < 0.05 or P < 0.01). CONCLUSION: Before and after Fructus Trichosanthis steamed, the change of fingerprint feature and changes of the component content provide experimental basis for further studies of Fructus Trichosanthis and its steamed products.

OBJECTIVE: To study the chemical constituents of different solvent extracts from Fructus Trichosanthis, and to study their antioxidant activity and protection of myocardial ischemia reperfusion injury in rats. METHODS: The chemical constituents in different solvent extracts were determined by UV-Vis spectrophotometry. Myocardial ischemia reperfusion model was made by ligation of left coronary artery in rats. Rats were randomly divided into 6 groups, with 6 rats in each group. The sham operation group and the model groups were respectively given normal saline, Fructus Trichosanthis (equivalent to crude drug 22.5 g·kg-1), compound Danshen dripping pill group(0.085 g·kg-1). Medicines were given once a day for 7 d. After the last drug 1 h, left coronary artery was ligated for 30 min and then reperfusion was established for 120 min by removing the ligation. During this time, ECG was recorded. At the end, animals were euthanized. Blood was collected to evaluate the contents of CK-MB, MYO-MB, cTnT. The heart was removed and fixed to observe the changes of myocardial tissue by optical microscope. RESULTS: Compared with the water extract group and the alcohol extract group of Fructus Trichosanthis, the total amino acids content of Fructus Trichosanthis dichloromethane extract was not detected, but the content of total flavonoids is higher (P < 0.01). Compared with the water extraction liquid of Fructus Trichosanthis, the antioxidant properties on DPPH radical of Fructus Trichosanthis dichloromethane extract group is lower (P < 0.01), and the antioxidant activity on-OH free radical of the alcohol extract of Fructus Trichosanthis group is lower (P < 0.01). Compared with model group, the elevation of ST segment of electrocardiogram was significantly suppressed in each group during reperfusion (P < 0.01 or P < 0.05). The plasma CK-MB, cTnT and MYO-MB in water extract group were significant lowered (P < 0.05). CONCLUSION: The extraction of Fructus Trichosanthis is able to decrease the production of oxidants. The water extract of Fructus Trichosanthis could ameliorate myocardial ischemia reperfusion injury.

OBJECTIVE: To study the effects of rice wine on the metabolism of 3, 29-dibenzoyl karounitriol as the main active component in Gualou-Xiebai in human intestinal flora, exploring the scientific connotation of Gualou-XiebaiBaijiu Decoction from the angle of metabolism. METHODS: The metabolic rate of 3, 29-dibenzoyl karounitriol in Gualou-Xiebai rice wine solution and aqueous solution at different time points were compared,respectively,by intestinal flora experiments. RESULTS: Compared to Gualou-Xiebaiaqueous solution group, the metabolic rate of 3, 29-dibenzoyl karounitriol in Gualou-Xiebai rice wine solution group decreased significantly (P<0.05). CONCLUSION: Rice wine as cites drug can inhibit the metabolism of 3, 29-dibenzoyl karounitriol in Gualou-Xiebai.

Objective:To explore the way and feasibility of carrying out developmental-behavioral pediatrics(DBP) teaching for medical students on probation.Method :To divide 230 medical students on probation into experimental group(120 students) and control group(110 students).The former group spent four class hours in child developmental-behavioral outpatient department and another four hours in sensory integrative therapy room to assist doctors in their training work as volunteers,and their rest probation contents were the same as those of the latter group which had classes according to teaching outline of pediatrics.Test scores of pediatrics of the two groups were compared.Questionnaires filled in by students of experimental group were analyzed.Results of the two groups,scores of pediatrics were not different,which showed that scores weren't influenced by extra teaching contents.Results of questionnaires showed that teaching of DBP was welcome to students,and they had made advances in relative knowledge and their overall quality.Conclusion: It is necessary to carry out DBP teaching.The teaching way of this study has got good effect and is feasible.

Objective To study the similarity of chemical fingerprints of different proportion of Gualoupi-Gualouzi and Fructus Trichosanthis, so as to provide the theoretical basis for the reasonable proportion of Gualoupi-Gualouzi. Methods The HPLC fingerprints and the common patterns of 6 the proportion of the 6 kinds of compatibility (30∶70, 35∶65, 40∶60, 45∶55, 50∶50 and 55∶ 45) of Gualoupi-Gualouzi and Fructus Trichosanthis were established, and the similarity of chemical fingerprints of different compatibility proportion of Gualoupi-Gualouzi and Fructus Trichosanthis between their common patterns were compared. Results Compared with the common pattern of Fructus Trichosanthis, the similarities of the common patterns of chemical fingerprints of the above 6 kinds of compatibility proportion of Gualoupi-Gualouzi were 0.8808±0.0407, 0.8930±0.0236, 0.8995±0.0195, 0.9033±0.0190, 0.9363± 0.0082 and 0.8904±0.0144, respectively. Among them, Gualoupi-Gualouzi ratio of 50∶50 had the highest similarity, and compared with other groups, the differences were statistically significant (P<0.05 or 0.01). Conclusion The optimum proportion of Gualoupi- Gualouzi can be determined by the fingerprint technology, which provides the theoretical basis for the reasonable proportion of Gualou⁃pi-Gualouzi.

Objective To verify the protective effects of Gualou Xiebai dropping pills(GX) on myocardial ischemia-reperfusion injury(MIR.I) in rats. Methods After screening the qualified SPF rats were randomly divided into four groups, with 12 in each group. Sham-operated group and model group rats were respectively treated with normal saline, and rats in GX group and compound Danshen dropping pills group were given the corresponding dropping pills equivalent to 22.5 g/kg and 85.05 mg/kg of the crude herb, respectively. All groups were administered once a day for 7 successive days. One hour after the last administration, the MIRI models were produced by occluding the left coronary artery for 30 min and releasing the occlusion for 120 min. The changes in ST segment were observed, and the contents of creatine kinase-MB (CK-MB), cardiac troponin-T (CTNT) and myoglobin (MYO) in blood plasma were measured. The pathological changes in myocardial tissues were observed by optics and electric-microscope and the percentage of myocardial infarction was measured by detecting the content of Evan blue in myocardium. Results Compared to normal saline in the model group, GX had antagonism to ECG S-T segments elevation in rats with myocardial ischemia, while the contents of CK-MB, MYO, and CTNT in blood plasma declined significantly (P<0.05 or P<0.01); the disorder condition of myocardial cell fiber arrangement was improved, and edema between myocardial tissue and cells was relieved; the inflammatory cell infiltration and myocardial necrosis in cardiac allograft were significantly alleviated, and the percentage of myocardial infarction was decreased significantly (P< 0.01). Conclusion GX may play an important protective role against the MIRI in rats.