Anti-Infective Agents ; administration & dosage ; Clinical Competence ; Hematologic Diseases ; Hospitals, Special ; manpower ; Humans ; Medical Staff, Hospital

Acupuncture Therapy ; instrumentation ; methods ; Adult ; Aged ; Female ; Hot Temperature ; Humans ; Male ; Middle Aged ; Needles ; Spondylosis ; therapy ; Treatment Outcome ; Young Adult

Acute Disease ; Combined Modality Therapy ; Humans ; Pancreatitis ; therapy ; Pancreatitis, Acute Necrotizing ; therapy

Objective To investigate distributions and drug resistance of pathogens in cirrhotic ascites comphcated with spontaneous bacterial peritonitis (SBP). Methods Results of bacterial culture and drug sensitivity tests of 117 aacitie specimens from cirrhotic patients with SBP were analyzed retrospectively. Results From the 117 ascitie specimens, 117 bacterial strains were isolated, among which 71 (60.68%)were gram-negative bacteria, 45 (38.46%) were gram-positive bacteria. The isolated strains were highly resistant to antibacterial agents, especially for Escherchia coli and Staphylococcu, the resistances to most antibiotics were above 60%. Conclusions High drug resistances were common for pathogenic bacteria in aacites of liver cirrhosis patients complicated with SBP.

Objective To investigate the degenerative changes in the inner rat retina after photic injury. Methods After 24 hour-dark adaptation, sixty Lewis rats were exposed in a ventilated green plexiglass chamber that transmitted continuous green light between 480-520 nm with an intensity of 900～1 000 lx. After 24-hour exposure, the rats stayed in darkness and were sacrificed after 1 day, 3,7 or 14 days. The neurons in the inner retina were marked by immunohistochemical technique and observed by light and electronic microscope. Results The apoptotic photoreceptor cells were noted after photic injury. The degeneration and decreasing number of rod bipolar cells were found after 3 days; the edema of horizontal cells occurred after 1 day but ameliorated gradually; decreasing number of amacrine cells was found after 1 day; sustained edema of ganglion cells and prolifeeration of the M?ller cells were found after photic injury. Pyknotic and edematous neruronal degenerations of inner retina were found in ultrastructural study. Conclusion The neurons in the inner retina as well as M?ller cells are involved in the degeneration after photic injury. Different neurons manifest different patterns of degeneration.

Objective: To investigate the effect of oat on blood glucose、insulin and serum lipid in aged patients with type-2 diabetes mellitus.Methods: Blood glucose(FBG,PBG),insulin(F-INS,P-INS) and serum lipid(TG,TC) were determined in eighty aged patients with type-2 diabetes mellitus before and after 50-100 g oat intake. Results: FBG,PBG and P-INS were decreased significantly(P

Objective: To investigate the relationship between attributional style and personality. Methods: We measured undergraduates with Attributional Style Questionnaire(ASQ)and Sixteen Personality Factor Questionnaire(16-PF).Results: Students with high-sensitivity, high-insecurity, high-tension, anxiety, not tough poise and low marks in Mental Health are apt to have negative attributional style. These personalities are the nuclear personalities of negative attributional style. Students with high-suspiciousness, high-insecurity,anxiety and low marks in Academic Achievement and Adjustment are apt to attribute fatefully. Conclusion: The nuclear personalities for negative attributional style are high-sensitivity, high-insecurity, high-tension, anxiety, not tough poise and low marks in Mental Health.

Objective To observe the therapeutic effect of Pinggan Yishen Ditan Decoction (Liver-Calming Kidney-Boosting Phlegm-Flushing Decoction) for hypertensive obesity and its possible mechanism.Methods Sixty cases of hypertensive obesity were randomly divided into Chinese medicine group (Chinese medicine was added on the basis of treatment in control group) and control group.The treatment course lasted for 8 weeks.Blood pressure (BP),body weight,waist circum-ference(WC),waist-to-hip ratio(WHR),metabolism parameters,fasting insulin(FINS) and Leptin were observed and compared before and after treatment.Results There was significant difference in SBP,DBP,BMI,WC,WHR,FPG,TC,TG,HDL-C,LDL-C,FINS,Leptin and ISI after treatment in Chinese medicine group (P

AIM To study the hepato-protection of indole-3-carbinol (I3C), one of glucosinolate compounds in cruciferous vegetables, on ethanol-induced hepatotoxicity. METHODS Ethanol 50 mmol?L -1 was used to induce hepatotoxicity in precision liver-cut slices of rats. The release of glutamic pyruvic transaminase(GTP), glutamic oxaloacetic transaminase (GOT), lactate dehydrogenase (LDH) and glutathione S-transferase (GST) into culture medium, and aniline hydroxylase (ANH) and alcohol dehydrogenase (ADH) in cytosol of hepatocytes in the precision liver-cut were measured with different doses of I3C. The histopathological observation of liver slices was also done to evaluate the effect of I3C. RESULTS The release of GTP, GOT, GST and LDH in the medium increased significantly when the slices were treated with 50 mmol?L -1 ethanol for 4 h. I3C at concentrations of 100～400 ?mol?L -1 effectively reduced the release of GTP, GOT, GST and LDH in the medium of ethanol-treated slices. Meanwhile, the activities of cytosol ANH and ADH decreased to the normal levels. The histopathological changes of liver slices induced by ethanol were lessened by I3C treatment. CONCLUSION I3C can markedly protect rat liver slices from ethanol-induced hepatotoxicity, which partly results from the change of ethanol metabolism in liver.

Objective To study the changes in the expression of GABA and its receptors in the dorsal horn of rat spinal cord induced by hind paw formalin injection. Methods Twelve healthy male SD rats weighing 300-325 g were randomly divided into two groups: (A) control group (n=6) and (B) peripheral inflammatory hyperalgesia group in which 5% formalin 50?l was injected subcutaneously into the plantar region of right hind paw to produce persistent pain(n=6). The animals were killed 24h after formalin injection. L_(4-6) segment of the spinal cord was removed. The number of GABA immuno-reactive cells was examined by immuno-cytochemistry technique and the exprpession of GABA_(A?3) and GABA_(B1) receptor mRNA in the spinal dorsal horn were determined by in situ hybridization. Results In control group(A), GABA immuno-reactive cells and GABA_(A?3) and GABA_(B1) receptor mRNA were observed throughout the lumbar spinal dorsal horn. The density of GABA immuno-reactive cells and GABA_(B1) receptor mRNA was highest in the superficial laminae Ⅰ-Ⅲ; while the GABA_(A?3) receptor mRNA was evenly distributed in the spinal dorsal horn. In group B, 24h after formalin injection there was a significant increase in the GABA immuno-reactive cells in the ipsilateral spinal dorsal horn. The expression of GABA_(A?3) and GABA_(B1) receptor mRNA were significantly increased in bilateral dorsal horn compared to group A. The three parameters were positively correlated and their locations in the spinal dorsal horn were not significantly different from those in group A. Conchusion GABA and its receptors may play an important role in nociceptive modulation.