1.Application study of PET-CT image fusion in conformal radiotherapy of non-small cell lung cancer
Journal of International Oncology 2011;38(6):450-453
The application of PET-CT could not only play a part in the diagnosis of clinical stage for NSCLC on PET, but also make full use of the discrimination of anatomical structure on CT. The precision of target contouring could be increased and the normal tissue and risk organ are also protected efficiently.
2.Conservative Surgery by Laparoscopy for Ectopic Pregnancy
Chinese Journal of Minimally Invasive Surgery 2005;0(10):-
Objective To investigate the efficacy of conservative treatment with laparoscopy for patients with ectopic pregnancy.Methods From January 2000 to December 2005,76 patients with ectopic pregnancy received conservative treatment by laparoscopy in our hospital.The data of the patients were analyzed retrospectively.Results In this series,fenestration of the Fallopian tube was performed on 42 cases,salpingostomy on 21,fimbrial expression on 8,and segmental resection and end-to-end anastomosis on 5.Salpingography showed that the Fallopian tube was patent after the operation in all of the patients.The mean operation time was(58.5?19.4) minutes(ranged from 35 to 105).The serum level of ?-hCG decreased to a normal range in 3 weeks postoperation.Follow-up was available in 61 cases for 1 year,during which 35 of the cases(57.4%,35/61) had intrauterine pregnancy;2(3.3%) had spontaneous abortion,and 2(3.3%) showed ectopic pregnancy;the other 22(36.1%) had no pregnancy.Conclusions Conservative treatment by laparoscopy is safe and effective for ectopic pregnancy.To avoid repeated ectopic pregnancy,?-hCG monitoring after the operation is necessary.
3.How to deal with cerebral palsy in 21st century--a new epoch in clinic treatment.
Chun-Yu TIAN ; Li-Ge LENG ; Zeng-Min TIAN
Chinese Journal of Applied Physiology 2014;30(6):511-515
The aims of this paper were to define (1) criteria of cerebral palsy; (2) classification of cerebral palsy; (3) etiology, neuroimaging, and epidemiology of cerebral palsy; (4) different kinds of treatments of cerebral palsy. Data were drawn from an international survey of PUBMED (1994-2014) and CNKI (1994-2014). An expert panel used a consensus building technique. The10-point Jadad scale was used to assess the quality of the trials based on the following items, including allocation sequence generation, randomization concealment, methods of blinding, and descriptions of withdrawals and dropouts. Our clinical experience was also summarized. Below is a summary. (1) Further work is warranted to reach agreement for the classification of cerebral palsy. (2) A worldwide prevalence of 1.5-4.0 per 1 000 live births, with an average lifetime cost of 1 million dollars per person in the United States, while it is 1.8-6.0 per 1000 live births in China. (3) Comparison of clinical efficacy of different treatments. In this review, the current advances in different kind of treatments of brain injury are discussed with specific relevance to cerebral palsy.
Cerebral Palsy
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classification
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diagnosis
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therapy
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China
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Humans
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Prevalence
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United States
4.Basic fibroblast growth factor promotes the differentiation of rat bone marrow mesenchymal stem cells into Leydig cells.
Xiu-wen YAN ; Chun LIU ; Fei TIAN
National Journal of Andrology 2015;21(6):494-499
OBJECTIVETo explore the role of the basic fibroblast growth factor (bFGF) in the directional differentiation of bone marrow mesenchymal stem cells (BMSCs) into Leydig cells.
METHODSAfter purification and identification, we inoculated the third-generation BMSCs of SD rats onto a six-orifice board and then randomly divided them into groups A (normal saline control), B (human chorionic gonadotropin [hCG] + platelet-derived growth factor [PDGF] induction), C (hCG + PDGF + 5.0 ng/ml bFGF induction), D (hCG + PDGF + 10.0 ng/ml bFGF induction), and E (hCG + PDGF + 20.0 ng/ml bFGF induction). On the 7th, 14th and 21st day of induction, we observed the morphological changes of the cells and measured the level of testosterone (T) and expression of 3 beta hydroxy steroid dehydrogenase (3β-HSD) in the supernatant by immunofluorescence staining.
RESULTSAfter induction, the BMSCs of groups B, C, D, and E exhibited microscopic features of enlarged size, inter-connection, long-shuttle or irregular shape, adherent growth, and large round nuclei, all characteristic of Leydig cells. With the prolonging of time and enhanced concentration of bFGF, gradual increases were observed in the T level and the count of 3β-HSD-positive BMSCs in the four induction groups, with statistically significant differences between group B and groups C, D, and E (P < 0.05), as well as between group C and groups D and E (P < 0.05), but not between D and E (P > 0.05).
CONCLUSIONThe bFGF has an obvious promoting effect in the in vitro induced differentiation of rat BMSCs into Leydig cells.
Animals ; Bone Marrow Cells ; cytology ; drug effects ; metabolism ; Cell Differentiation ; Cells, Cultured ; Chorionic Gonadotropin ; metabolism ; Fibroblast Growth Factor 2 ; pharmacology ; Humans ; Leydig Cells ; cytology ; Male ; Mesenchymal Stromal Cells ; cytology ; drug effects ; metabolism ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Testosterone ; metabolism
5.Effects of Microwave Radiation Exposure of Pregnant Mice on Offspring
Juan TIAN ; Yuanzhen ZHANG ; Chun ZHOU
Journal of Environment and Health 1989;0(06):-
Objective To study the relationship between microwave radiation exposure during pregnancy and the offspring health. Methods The pregnant Kunming mice were divided into 3 groups and exposed to microwave radiation of 0, 570 and 1 400 ?W/cm2 respectively. The bone marrow cells of the offspring were collected for the determination of chromosome aberration (CA), sister chromatid exchange (SCE) and micronucleus frequency (MNF). Results SCE showed a significant change in high intensity group, as for CA and MNF, no significant change was seen compared with the control. Conclusion Microwave radiation exposure during pregnancy may induce germ plasm damages in the offspring.
6.Reformation of Fundamental Medicine Education as Development of EMB
Chun-Xiang LI ; Mao-You TIAN ;
Chinese Journal of Medical Education Research 2006;0(07):-
The conception of evidence-based medicine (EMB) has a strong influence on the medical world since it built up.This concept has been widely used in all sorts of clinic and basic medical research,however,it is little reported to be practised in our fundamental medical education.As for it,this article will briefly analyze the importance of introducing the idea into fundamental medical education,and for one thing,suggest some more reform measures for medical teaching staff.
7.TCM Tongue-appearance Analysis and Pathology Discussion on 223 HIV/AIDS Patients in Different Periods of Disease
Xin CHEN ; Chun TIAN ; Yuzhen CHEN
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(12):-
Objective To analyze and sort the character of symptom and pathology of 223 HIV/AIDS patients in Yunnan Provine. Methods Sort the clinical periods into groups subject to 223 HIV/AIDS patients who voluntarily accept the consultations and treatments for the first try in Yunnan province, then observe their tongue-appearance accordingly. Results By separately observing the color, shape and aspect of the tongue and tongue-fur which belongs to patient during AIDS period and asymptomatic period, discovered that the pathological tongue-fur appearance of all clinical periods trends to greasy, thereinto, in period of AIDS, 47.45% were white-greasy, 16.06% were yellow-greasy, 8.02% were yellow and thin. In terms of color of tongue, 46.71% of the total samples were light pink and white, 32.12% were red, and 21.17% were purple and gore. In period of asymptomatic, most of the tongue-appearance was light pink and thin white, which obviously exceeded the AIDS period. It indicates that the higher level of immunity, the more normal appearance of tongue can be expected. Group A (200≤CD4
8.Clinical Analysis on 38 Cases of Laparoscopic Operation in the Treatment of Large Ovarian Cysts
Hong ZHOU ; Chun TIAN ; Aihua LI
Chinese Journal of Minimally Invasive Surgery 2005;0(08):-
Objective To investigate the feasibility of laparoscopic operations in treating large ovarian cysts.Methods A total of 38 patients with large ovarian cysts underwent laparoscopic cyst cystectomy or adnexectomy on the affected side from December 2002 to October 2005.The 2nd or 3rd trocar(usually on the tumor side)was inserted into the cyst,the fluid in which was drained with a suction unit.The cyst was then removed after being elevated toward the incision,which was extended to 20 mm.Results Large ovarian cysts in the 38 cases were all removed successfully,including cystectomy in 28 cases and adnexectomy in 10 cases respectively.No complication occurred.The follow-up period was 1-24 months(mean:10 months)for 35 cases.No abnormal findings in pelvic examination and B ultrasonography were observed.Conclusions Laparoscopic operation is a safe and feasible therapeutic method for large ovarian cysts.
9.Effect of down-regulation of nucleolin on adriamycin-induced apoptosis and inhibition of proliferation in breast cancer cells.
Xiao-cai TIAN ; Xian-ling LIU ; Chun-hong HU
Chinese Journal of Oncology 2010;32(4):271-272
Antibiotics, Antineoplastic
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pharmacology
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Apoptosis
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drug effects
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Breast Neoplasms
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metabolism
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pathology
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Cell Line, Tumor
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Cell Proliferation
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drug effects
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Down-Regulation
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Doxorubicin
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pharmacology
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Female
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Humans
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Oligonucleotides, Antisense
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pharmacology
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Phosphoproteins
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metabolism
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RNA-Binding Proteins
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metabolism
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Random Allocation