2.Effect of Psychological Stress on Rat Behavior and Condylar Cartilage
Journal of Kunming Medical University 2013;(9):1-6
Objective To establish the Sprague-Dawley rats psychological stress model and investigate the effect of the psychological stress on behavior and condylar cartilage of Sprague-Dawley rats. Methods Sprague-Dawley rats were randomly divided equally into psychological stress group, foot shock group and control group, and the foot shock group was only as sources of stimuli. The Sprague-Dawley rats psychological stress model was built, and the rats were killed at 1, 3 and 5 weeks respectively after stimulation. The behavior changes were observed by open field test,etc. The histology of condylar cartilage was observed by HE stain. Results Psychological stress model was effective in evaluating behavior changes. The pathological examination revealed structural changes of the condylar cartilage in the psychological stress group. The psychological stress groupⅡwas the most serious group with collagenous fibers disintegrated and gaps formed. Conclusion This rats model of the psychological stress shows great practicability and reproducibility. The long-term psychological stress can lead to SD rats behavior changes and temporomandibular joint condylar cartilage change.
3.Advance in glutathione S-transferase kappa 1
Journal of Central South University(Medical Sciences) 2017;42(4):468-475
Glutathione S-transferase kappa 1 (GSTK1) is a key regulator for adiponectin secretion and multimerization.In Caenorhabditis elegans,GSTK1 is involved in energy production and lipid metabolism.Meanwhile,the GSTK1 level is negatively correlated with obesity.It may alleviate the endoplasmic reticulum stress-mediated downregulation of adiponectin.Moreover,a polymorphism in human GSTK1 promoter is related with insulin secretion and fat deposition.Therefore,GSTK1 might be a novel target for the treatment of insulin resistance and the relevant metabolic diseases.
6. Optimization of urinary sample preparation process for real-time PCR detection of BK virus
Academic Journal of Second Military Medical University 2010;31(4):438-441
Objective: To develop an effective preparation method to improve the sensitivity and accuracy of real-time PCR in detection of BK virus's (BKV's) load in urine samples. Methods: A total of 24 samples documented as positive probes in primary detection were enrolled in this study. The candidate samples were prepared by 4 different approaches: unprocessed urine, BKV's DNA extracted from urine, 1:10 diluted urine, and 1:100 diluted urine; and then they were subjected to real-time PCR examination to obtain the viral load. The data obtained were analyzed by SPSS 11.0. Results: The four different preparation processes for urinary specimens had significant impact on detection results of real-time PCR. Three samples were negative in the unprocessed urine group and 66.7% of its samples had the lowest viral loads compared with the other three groups. Two samples in the 1:100 diluted urine group were negative and 79.2% of its samples had the highest viral loads, but its median load was similar to that of the 1:10 group. Viral gene was detected in all samples in the DNA extraction group and 1:10 diluted urine group, but the loss of the target gene was more severe in the DNA extraction group. Conclusion: The 1:10 diluted urine is better for real-time PCR detection of BKV's load, as it lose less viral gene and is more efficient, easy to perform and economical.
8.Pittsburgh Sleep Quality Index Investigation of Depressive Disorder Patients in the East-region of Shenyang
Jiang-ying WU ; Xiao-chun WANG ; Chun-lin LI
Chinese Journal of Rehabilitation Theory and Practice 2006;12(6):545-546
ObjectiveTo investigate sleep disorder of patients with depression and find out the regularity.Methods78 depression cases in the east-region of Shenyang were evaluated by Pittsburgh Sleep Quality Index (PSQI) questionnaire and mental health symptom checklist-90 (SCL-90). The results were contrasted with normal control group.Results63 depression cases (80%) had sleep disorder (PSQI scores≥8) and were significantly higher than normal control group. The PSQI evaluation showed that sleep quality, enter-sleep time, sleep efficiency, sleep disorder, hypnotige drugs, function of daytime and total scores of PSQI of the depression group were significantly higher than that of normal control group (P<0.05~0.01). The SCL-90 test showed that scores of somatic, obsessive, depressive, anxiety, phobia, paranoid and mental-illness, etc. and total quota of bad-sleep quality group were significantly higher than that of the fine-sleep quality group (P<0.05~0.01).ConclusionDepression patients have significantly descent in sleep quality, latter is often closely related to the mental psychotic expressiveness of somatic, obsession, depression, anxiety, phobia, etc.
9.A more sensitive method for the evaluation of visceral pain sensitivity in rat.
Yu CHEN ; Chun LIN ; Ai-Qin CHEN
Chinese Journal of Applied Physiology 2009;25(1):54-59
Abdominal Muscles
;
physiology
;
Abdominal Pain
;
diagnosis
;
physiopathology
;
Animals
;
Animals, Newborn
;
Colon
;
innervation
;
Electrodes
;
Electromyography
;
Female
;
Pain Measurement
;
methods
;
Pain Threshold
;
physiology
;
Rats
;
Rats, Sprague-Dawley
;
Viscera
;
Visceral Afferents
;
physiology
10.Superficial angiomyxoma of neck: a case report.
Chinese Journal of Pathology 2006;35(7):438-438
Adult
;
Diagnosis, Differential
;
Humans
;
Immunohistochemistry
;
Male
;
Myxoma
;
metabolism
;
pathology
;
surgery
;
Neck
;
Neoplasms, Muscle Tissue
;
pathology
;
Soft Tissue Neoplasms
;
metabolism
;
pathology
;
surgery
;
Vimentin
;
metabolism