Objective: To explore the biological effect of high frequency ultrasound and low frequency ultrasound on different layers in vitro swine adipose tissue. Methods: In vitro experiment was divided into two groups with working frequency of transducers being 6.5 MHz and 3 MHz. In 6.5 MHz group, ultrasonic power 2-5 W and irradiation time 5, 10, 20, 40, 60 and 80 s were adopted and cell activity was determined through MTT staining method and thus the area of coagulation necrosis was calculated. In 3 MHz group, biological effect induced by focused ultrasound on different layers of the swine adipose tissue was observed and the difference in result was discussed under same experiment parameters. Results:Lipolysis effect changes in an order with increasing ultrasonic power and irradiation time. 5 s irradiation time shows no obvious damage to the tissue, however shows obvious damage above 10 s. Under the same experiment parameters, deep adipose tissue (DAT) shows higher level of tissue damage than superficial adipose tissue(SAT). Only 3 MHz frequency can pass through the skin of thickness 5 mm at irradiation time≥40 s. Conclusion:Both high and low frequency ultrasound can effectively destroy adipose tissue.

On the grounds of multicultural coexistence system of Chinese University, this paper propose the basic elements management culture, namely integrity management, innovation management, intelligence management, fine management, system management, which referred to as primitive management culture. This paper also describes the meaning, and explores the function, values and application of primitive management culture. This paper advocates the development of the primitive management culture, and learns primitive management at the same time. We aim to cultivate talent with primitive management cultural qualities, and to achieve the dream of culture encouraged strong school, strong and prosperity nation with the production activities, social and natural science learning and practice.

AIM To study the hepato-protection of indole-3-carbinol (I3C), one of glucosinolate compounds in cruciferous vegetables, on ethanol-induced hepatotoxicity. METHODS Ethanol 50 mmol?L -1 was used to induce hepatotoxicity in precision liver-cut slices of rats. The release of glutamic pyruvic transaminase(GTP), glutamic oxaloacetic transaminase (GOT), lactate dehydrogenase (LDH) and glutathione S-transferase (GST) into culture medium, and aniline hydroxylase (ANH) and alcohol dehydrogenase (ADH) in cytosol of hepatocytes in the precision liver-cut were measured with different doses of I3C. The histopathological observation of liver slices was also done to evaluate the effect of I3C. RESULTS The release of GTP, GOT, GST and LDH in the medium increased significantly when the slices were treated with 50 mmol?L -1 ethanol for 4 h. I3C at concentrations of 100～400 ?mol?L -1 effectively reduced the release of GTP, GOT, GST and LDH in the medium of ethanol-treated slices. Meanwhile, the activities of cytosol ANH and ADH decreased to the normal levels. The histopathological changes of liver slices induced by ethanol were lessened by I3C treatment. CONCLUSION I3C can markedly protect rat liver slices from ethanol-induced hepatotoxicity, which partly results from the change of ethanol metabolism in liver.

Objective: High Intensity Focused Ultrasound (HIFU) is classified as the third category medical instruments, which means it require much more attention on its safety to patient. Methods: To achieve this goal, a safety monitor system was applied on “High Intensity Focused Ultrasound therapeutic equipment for Shallow tissue”. To design parallel port-based safety monitor method for high intensity focused ultrasound therapeutic system and make the bidirectional data flow. Results: By using parallel computation of FPGA, the safety monitor independently monitor several modules of the “Ultrasound therapeutic equipment”, and handles mal-function. A new EPP-Parallel port basic intelligent watchdog was introducing to monitor PC working state. Conclusion: The safety monitor system enhances the safety and reliability of the equipment, and provides helpful information for malfunction diagnose.

Carcinoma, Hepatocellular ; virology ; Genome, Viral ; Hepatitis B virus ; genetics ; Humans ; Liver Neoplasms ; virology ; Mutation

Annexins ; Humans ; Pulmonary Fibrosis ; pathology

Animals ; Curcumin ; pharmacology ; therapeutic use ; Humans ; Liver Cirrhosis ; drug therapy

Objective In order to explore the effectiveness of defluoride project on the control of endemic fluorosis in Yantai economic & technological development area. Methods Nine hundred forty-five children aged 7-12 were selected in 4 endemic fluorosis disease areas (Guxian,Bajiao,Dajijia Streets and Changjiang community) and the control area of Yantai economic & technological development area. Prevalence of children's dental fluorosis was determined by Dean method. Water fluoride content was measured in the 4 endemic fluomsis disease area by means of fluorides ion selective electrode. Results Childr dental fluorosis prevalence rate in Guxian,Bajiao,Dajijia Streets and Changjiang community was respectively 30.1% (55/183),41.2% (73/177),33.7% (66/196),31.8% (64/201),higher than those in the control area [13.3% (25/188)]; the median of water fluoride in the 4 disease areas was 1.84,3.46,2.39,2.10 mg/L,respectively,higher than the National Health on the control of endemic fluorosis is not obvious,Standard (1.00 mg/L). Conclusions Effect of the defluoride project in Yantai economic & technological development area needs to be improved.