Medicine, Chinese Traditional ; Reproductive Techniques, Assisted

Drugs, Chinese Herbal ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; methods ; Phytotherapy ; Reproductive Techniques, Assisted

Objective To investigate the immune privilege of cotransplanted parathyroid cells induced by testicular Sertoli cells expressing Fas ligand (FasL).Methods Different testicular Sertoli cells and allogeneic parathyroid cells were cotransplanted respectively. Allografts survival,cells component and the apoptosis of infiltrative lymphocytes in vitro were analyzed.Results The parathyroid cells transplanted alone were all rejected with the mean survival time of ( 17.22 ? 3.63 ) days. When parathyroid cells were cotransplanted with testicular Sertoli cells,survival time of allografts was prolonged. When the quantity of testicular cells was increased to 4?10 6,most animals remained normal serum calcium and PTH throughout the follow-up period ( P

Objective To explore the pattern and quantify the heat shock protein HSP)70 and HSP70 mRNA in Vero-E6 cells after infection with Hantann virus(HTNV).Methods The expres- sion of HSP70 and change of its mRNA level were detected by immunocytochemical staining,nucleic acid hybridization in situ and RT-PCR.Results In situ hybridization and RT-PCR were used to eval- uate the level of HSP70 mRNA during Hantaan 76-118 infection.HSP70 mRNA increased 0.5 h after infection,reached its peak by 12 h and gradually declined to steady state level by 72 h(vs.sham infec- ted group,P＜0.05).The expression of HSP70 protein induced by Hantaan 76-118 infection was e- valuated by quantitative immunocytochemical staining.HSP70 increased 0.5 h after infection,reached its peak by 12 h and decreased at 72 h after infection(vs.sham infected group,P＜0.05).Conclu- sions HSP70 can be induced directly by HTNV infection at both mRNA and protein levels,It pro- vides a basis for the further study of the pathogenesis,prevention and treatment of hemorrhagic fever with renal syndrome(HFRS).

Objective:To investigate the role of blockade of CD40 ligand-CD40 costimulatory pathway by anti-CD40L mAb on T lymphocytes typing and cytokines, and provide the experimental clues of inducing donor-specific T cell anergy.Methods:To monitor primary MLR, C57BL/6 H-2b spleen T cells were isolated as responder cells, and BALB/C H-2d spleen cell as stimulator cells. Anti-CD40L mAb was added to primary MLR cultures(termed anti-CD40L mAb group), and in control group(no anti-CD40L mAb). Incubated for 7 days, the cells responsiveness rates were detected by 3 H-TdR methods at the indicated time points, and supernatants were assayed for IFN-?,IL-2,IL-4,IL-10 cytokines levels by commercial ELISA kits. Day 5 MLR-cultured cells were assessed for the expressions of CD4, CD8, CD25, CD69, CD40L and CD45RA with flow cytometry(FCM).Cells proliferation for 5 days and cytokines in secondary MLR were assayed according above-mentioned method.Results:Blockade of the CD40-CD40L pathway by anti-CD40L mAb can induce the hyporesponsiveness to alloantigen in primary and secondary MLR culture. In primary MLR culture, the expressions of CD4 +T and CD8 +T cells and CD4 +CD25 +T, CD4 +CD69 +T, CD4 +CD40L +T, CD8 +CD25 +T and CD8 +CD69 +T cells in anti-CD40L mAb group were lower than that in control group ( P 0.05).The expression of CD4 +CD45RA + T in anti-CD40L mAb-cultured group was higher than that in control group( P

Crossbite of upper and lower second molars is a common malocclusion. It is difficult to treat such cases. 4 kinds of treatments according to different situations are recommended. One is a mutual elastics on the upper and lower teeth. The second method is to move upper and lower teeth by archwire lingually and bucally. The third is to extract the second molars, while the third molars drift automatically. The fourth is using mini-implant as an anchorage to move the teeth to correct position. This article evaluated the advantages and disadvantages of the 4 kinds of methods. We also discussed how to choose the appliances as well as what we should pay attention to treat this malocclusion.

Animals ; Coxsackievirus Infections ; metabolism ; Enterovirus B, Human ; Female ; Male ; Mice ; Myocarditis ; metabolism ; Myocardium ; metabolism ; Tumor Necrosis Factor-alpha ; metabolism

Objective To investigate the effect of Fas ligant (FasL) expression of testicular Sertoli cells on cotransplanted parathyroid cells. Methods Various numbers of rat testicular Sertoli cells with FasL expression and allogeneic parathyroid cells were cotransplanted. Allograft survival, change in cell components, apoptosis of infiltrative lymphocytes and parathyroid function were analyzed. Results The parathyroid cells when transplanted alone were all rejected, the mean survival time of allografts was (17?4)days. When parathyroid cells were cotransplanted with testicular Sertoli cells, the survival time of allografts was prolonged over 60 days. When the quantity of testicular cells was increased to 4?10~6, 6 of 8 rats maintained normal serum calcium and PTH levels throughout the follow-up period of 60 days (P

0.05). However, statistically significant changes of those data were found perioperatively in parathyroid gland excision group (P

Aim To evaluate the antianginal efficacy of trimetazidine in combination with other regular anti-ischemic drugs in the treatment of stable angina. Methods Twenty-two male cases with stable,effort-induced angina and positive exercise ECG test were treated with trimetazidine for 12 weeks.Exercise ECG test was examined again in the end of the study. Results There were obviously increased in exercise tolerance,total exercise workload after treatment(P