OBJECTIVE To observe the situation of Mycoplasma and Chlamydia trachomatis(Ct) infection in the patients with reproductive tract infection(RTI),and their antibiotic resistance analysis and therapeutic strategies.METHODS To analyze Ct antibody with monoclonal antibody immunogold filtration assay,and to detect Mycoplasma and their drug sensitivity with culture method.RESULTS From 581 specimens there were 42 cases who had Ct(7.2%),and 239 cases who had Mycoplasma(41.1%).The single infection of Ureaplasma urealyticum(Uu) was in 165 cases(28.4%) and of Mycoplasma hominis(Mh) in 18 cases(3.1%),respectively,Uu+Mh were in 39 cases(6.7%).Uu+Ct were in 17 cases(2.9%).The result of drug sensitivity showed that the drug resistance of Uu to erythromycin was 64.8%,to roxithromgcin was 62.6%.Mh was also with the similar tendency.The mixed infections rate was elevated.Doxycycline,minocycline,and josamycin had the higher bacteriostatic ability.CONCLUSIONS With the growing number of mycoplasma infections,and large-scale application of antibiotics themultidrug-resistance was occurred,so it is important to clinical reference to the selection of antibiotic susceptibility test results,and sufficient and adequate course of therapy to reduce the selection of resistant strains.Experimental evidence showed doxycycline,minocycline and josamycin can be used as the first choice for clinical medicine in mycoplasma infection.