Objective:To explore the influence of liquid for post-operative irrigation on the effect of ESS.Method:192 cases with chronic sinusitis and/or nasal polyps of groupIIstageII who had undergone the ESS from September,1998 to June,1999 and completed a3-month follow-up were divided randomly into 3 groups to accept 3 different kinds of irrigation liquid.The evaluation of curative effect was according to the index of HaiKou ESS-97.Result:The curative effect of Herba Houttuyniae group was better than the other two.Conclusion:The post-operative care is important and the irrigation liquid can affect the effect of ESS.

Objective: To explore the nature of voltage dependent ion channels and basic electrophysiological characteristics of cochlear spiral ganglion neurons of apical and basal turn by patch clamp techniques of whole cell configure on murine spiral ganglion neurons. Method: Different voltage dependent ionic currents were recorded with patch clamp techniques of whole cell configure on the condition of different internal electrode solution,blockers and stimulus protocol. Result: Inward sodium channel current(I_(Na)), hyperpolarization-activated inward cationic current(I_h) ,outward delay rectification potassium current (I_k) and outward transient potassium current (I_A)were recorded , significant difference of electrophysiological characteristics of I_A and I_k was found between apical and basal turns(P＜0.05). Conclusion: Various ionic currents are recorded,which shows that spiral ganglion neurons have the base of ionic channels to complete formation,conduction and modulation of action potential for auditory information transduction, the difference of electrophysiological characteristics between apical and basal turns contributes to the course of hearing formation.

OBJECTIVE: To study the clinical feature and treatment of extracranial and intracranial complications caused by otitis media. METHOD: Three hundred and twenty patients of acute and chronic otitis media were admitted to our department between 2005 and 2014. Among them, 34 patients were diagnosed with extracranial and intracranial complications. The clinical features and treatment outcome were retrospectively studied. Of the 34 patients associated with complications, 25 had a single complication,8 had two complications and 1 had three complications. Complications included labyrinthitis in 14 cases, facial paralysis in 11, postauricular subperiosteal abscess in 6, Bezold abscess in 1, thrombophlebitis of sigmoid sinus in 2, otitis meningitis in land otogenic brain abscess in 8. RESULT: Thirty-three patients were cured or improved and 1 patient died. CONCLUSION Due to the widespread use of antibiotics, the clinical manifestations of extracranial and intracranial complications of otitis media become more hidden and atypical. The surgery is the primary treatment method.
Brain Abscess ; complications ; Chronic Disease ; Facial Paralysis ; complications ; Humans ; Labyrinth Diseases ; complications ; Mastoiditis ; complications ; Meningitis ; complications ; Otitis Media ; complications ; physiopathology ; Retrospective Studies ; Treatment Outcome

Objective The purpose of this study w as to obtain tympanometric norms in Sichuan young adult population and compare the results with data obtained for a non-Hispanic Caucasian population. Methods Normative values for peak compensated static acou stic admittance(Peak Ytm), acoustic equivalent volume(Vea), and tympanometric wi dth(TW) were obtained for 102 Sichuan young adults. Results Significant differences were fo und between Roup′s results and ous for Peak Ytm, Vea and TW. The present study showed lower Peak Ytm, smaller Vea and larger TW values overall. Gender differences were noted for Peak Ytm and Vea in Sichuan subjects . Male had higher Peak Ytm and bigger Vea values than female. Conclusion We should establish our own tympanometric norm s to guide our audiological clinical work.

Objective To explore the influence of gentamycin on murine cochlea spiral ganglion neurons' electrophysiological properties and its significance.Methods Using whole-cell voltage clamp technique, we studied gentamycin's influence on the peak currents of the potassium and sodium ion channels on cell membranes of acutely dissociated murine spiral ganglion neurons,the relationship to gentamycin's concentration in extracellular fluid, and the currents' recovery after gentamycin being washed out.Results Gentamycin could inhibit voltage-dependent potassium channels, but it couldn't inhibit voltage-dependent sodium channels. Gentamycin's inhibitation on potassium currents had dose-dependence with gentamycin's concerntration in extracellular fluid and the currents recoverd incompletely after gentamycin being washed out.Conclusion This research explained the ototoxic mechanism of gentamycin through its action on keeping from spiral ganglion neurons' potassium ion channels from the electrophysiological aspect, and set a foundation for further research.

Objective To investigate the clinical procedural performance of CT-guided needle biopsy for peritoneal lesions.Methods CT-guided needle biopsy was performed in 84 consecutive patients (M : F=26 : 58) with peritoneal lesions.Results Among 84 cases,60 lesions were malignant (55 metastatic tumor,4 mesothelioma,1 lymphoma) and 24 were benign (11 tuberculosis, 13 inflammation).3 cases failed to get clear pathologic diagnosis because of lost data.Diagnostic accuracy was 91.7% (77/84).The major complications were noted in 3 patients(1 with bleeding,2 ascites exosmosis).Conclusion CT-guided needle biopsy for retroperitoneal lesions is highly practical and useful,should be considered complementary in the investigation of abdominal lesions.

OBJECTIVE: To establish an integrated database for laryngeal cancer, and to provide an information platform for laryngeal cancer in clinical and fundamental researches. This database also meet the needs of clinical and scientific use. METHOD: Under the guidance of clinical expert, we have constructed a web-based integrated clinical database for laryngeal carcinoma on the basis of clinical data standards, Apache+PHP+MySQL technology, laryngeal cancer specialist characteristics and tumor genetic information. RESULT: A Web-based integrated clinical database for laryngeal carcinoma had been developed. This database had a user-friendly interface and the data could be entered and queried conveniently. In addition, this system utilized the clinical data standards and exchanged information with existing electronic medical records system to avoid the Information Silo. Furthermore, the forms of database was integrated with laryngeal cancer specialist characteristics and tumor genetic information. CONCLUSION The Web-based integrated clinical database for laryngeal carcinoma has comprehensive specialist information, strong expandability, high feasibility of technique and conforms to the clinical characteristics of laryngeal cancer specialties. Using the clinical data standards and structured handling clinical data, the database can be able to meet the needs of scientific research better and facilitate information exchange, and the information collected and input about the tumor sufferers are very informative. In addition, the user can utilize the Internet to realize the convenient, swift visit and manipulation on the database.
Databases, Factual ; Internet ; Laryngeal Neoplasms ; Software ; User-Computer Interface

OBJECTIVE To evaluate the quality of follow-up in Chinese Archives Of Otolaryngology-Head And Neck surgery and offer evidence for the improvement of follow up. METHODS Chinese Archives Of Otolaryngology-Head And Neck surgery (January 1994 to December 2005) were searched,and clinical materials of follow-up was identified and analyzed. RESULTS Seventy-six issues were referred to and one hundred ninety-nine papers were finally identified and analyzed. CONCLUSION Although there are major problems on follow-up in Chinese Archives Of Otolaryngology-Head And Neck Surgery and Archives Of Otolaryngology-Head And Neck Surgery,much improvement has been made on the quantity and quality of clinical material follow-up in Chinese Archives Of Otolaryngology-Head And Neck Surgery.

OBJECTIVE To investigate the epidemic features of allergic rhinitis at out-patient in western area of China(Chengdu in Sichuan province, Chongqing, Urumchi in Xinjiang province, Nanning in Guangxi province). METHODS A questionnaire survey on AR, rhinology examination and skin prick test were carried out among 2267 AR patients at out-patient, from Mar. 2005 to Dec. 2006. RESULTS The incidence rate of sneezing、runny nose、stuffy nose、itchy nose were 88%, 89.19%, 85.58%, 83.58% respectively. 43.80% of AR patients had inherent predisposition that 25.50% had hereditary AR and 13.45% had hereditary asthma. 46.98% of AR patients had other allergic diseases, among them 10.50% combined with asthma. According to the classification criteria of Haikou in 1997 and ARIA in 2001, there were mainly seasonal AR and mild intermittent AR in Urumchi area, while perennial AR and moderate-severe persistent AR in other areas. There was a significant impairment in Quality of life that 50% were always upset and 40% didn't sleep well and more than 10% had emotional affections. CONCLUSION We had got epidemic data such as characteristics of clinical presentation of AR at out-patient in western of China. Strategy of prevention and treatment of AR should be worked out according to the epidemic feature of AR.

OBJECTIVETo evaluate the efficacy of combined interleukin (IL)-2 gene and IL-12 gene therapy in murine model with head and neck squamous cell carcinoma (HNSCC).

METHODSHNSCC model was established in the mouth floor of C3H/HeJ mice with SCCVII cell line. Lipid-IL2 and lipid-IL12 plasmid complexes were introduced either alone or in combination into the tumor by direct intratumor gene injection. Tumor size was measured before and after the treatment to evaluate the response of the different treatment and control groups. Enzyme-linked immunosorbent assay (ELISA) was used to measure the IL-2 and IL-12 expression. Lactic dehydrogenase (LDH) assay was used to evaluate the activity of Cytotoxic T-Lymphocyte (CTL) and natural killer (NK) cells.

RESULTSGrowth of HNSCC was significantly inhibited in combined IL-2 and IL-12 gene therapy group as compared with the other groups (P < 0.01). Increased level of IL-2 and IL-12 protein expression was found in both combined and single treatment groups. Greater activity of CTL and NK was also observed in these two groups as compared with the controls.

CONCLUSIONBoth IL-2 and IL-12 gene therapy is able to inhibit the growth of HNSCC and induce the host antitumor immune response efficiently in the murine model. Combination of the two in gene therapy may be additive or synergistic in antitumor effect.

Animals ; Carcinoma, Squamous Cell ; immunology ; physiopathology ; therapy ; Disease Models, Animal ; Female ; Genetic Therapy ; methods ; Head and Neck Neoplasms ; immunology ; physiopathology ; therapy ; Interleukin-12 ; genetics ; Interleukin-2 ; genetics ; Killer Cells, Natural ; immunology ; Mice ; Mice, Inbred C3H ; T-Lymphocytes, Cytotoxic ; immunology