OBJECTIVE To explore the infection control process in neonatal care unit by using evidence-based medicine(EBM).METHODS Under the guidance of EBM and process management theory,investigating and collecting relevant materials on hospital infection,searching the related literatures from the EBM websites(MEDLINE database,etc) and undertaking comprehensive evaluation,to establish the infection control process in neonatal care unit,in connection with practice,then using it in internal medicine and surgery wards.RESULTS After using infection control process,neonatal infection rate was reduced to 1.87%.Hygienic monitoring index of incubator,ventilator tube,pipe atomization,hand,air and handwashing compliance of medical staff were effectived changed(P