Objective:To research on the immune recognition mechanism of synthetic pyrethroids and generic specific antibody.Methods:We studied on quantitative structure-activity relationship ( QSAR ) of synthetic pyrethroids and their analogs as well as antibody activity ( IC50:fifty percent inhibition concentration ) using stepwise multiple linear regression method.Based on calculating structure descriptors of synthetic pyrethroids and their analogs , two-demensional QSAR ( 2D-QSAR ) model was established.The main factors affecting antibody activity were screened using 2D-QSAR,and predictive ability of QSAR models were evaluated by the method of leave-one-out( LOO) cross-validation.Meanwhile, the structure parameters of synthetic pyrethroid fragments were calculated and then analyzed using partial least squares ( PLS) assay.And then hologram QSAR ( H-QSAR) model was constructed on molecular substructure and antibody activity.The fragments contribution to antibody activity were illustrated by encoding different colors.Results:Decision coefficent (R2) of 2D-QSAR model and HQSAR model were 0.920 and 0.917 individually,cross-validation coefficient ( Q2 ) of two QSAR models were 0.875 and 0.660 respectively ,which showed two models had good predictive abil-ity.The result from 2D-QSAR model was also obtained that smaller was hydrophobicity of pyrethroids , easier was recognized by antibody.In addition,the optimum HQSAR model was constructed after we tried many combinations of these parameters .The fragment size in optimum HQSAR model was between 4 to 10,a hologram length was 61,optimum principle component was 4,and the fragment type of B/C/Ch was selected.However ,the fingerprint encoded results of synthetic pyrethroids weren′t consistent completely with exper-imental IC50 values.Conclusion:Hydrophobicity of synthetic pyrethroids is the largest correlation factors in antibody recognization .

OBJECTIVETo observe the immune reaction in the mixed culture of host lymphocytes with allogenic and host endothelial cells.

METHODSThe host epithelial cells and lymphocytes from burn patients and allogenic epithelial cells were mix-cultured in different ratios, so as to simulate the local immune micro-environment of host skin island in intermingled skin grafting. In addition, the cells from normal human subjects were also mix-cultured as control. The lymphocyte cpm values were detected by (3)H-TdR and HLA molecules and T cell subgroup were determined by immunohistological technique.

RESULTS(1) The lymphocyte proliferation reaction could be effectively inhibited by the epithelial cells from burn patients but not from normal control. (2) The inhibition of host lymphocyte proliferation could not be mediated by the HLA-DQ molecules of epithelium from burn patients. (3) The positive expression rate of HLA-DR of epithelia from burn patients was evidently higher that that from normal control (P < 0.05), (4) The CD8 expression of lymphocyte in burn patients was significantly higher than that in normal control (P < 0.01), while the CD4 expression in burn patients was lower than that in normal control (P < 0.01). But there was no obvious difference of the CD3 expression between patients and normal subjects (P > 0.05).

CONCLUSIONThe lymphocyte proliferation reaction could be obviously inhibited by the host epithelium, which might be related to the specific immune state of the host lymphocytes and epithelium of burn patients.

Cell Communication ; immunology ; physiology ; Cell Culture Techniques ; Cell Division ; Epithelial Cells ; immunology ; physiology ; Humans ; Lymphocytes ; immunology ; physiology ; Skin Transplantation ; immunology

Objective To explore the perioperative psychological behavior interventions for patients with postoperative anxiety and pain of gastrointestinal tumor. Methods We selected 110 patients with gastrointestinal cancer during surgical treatment from gastrointestinal surgery department of Anhui provincial hospital from April 2014 to October 2014 and divided them into experimental group (n=58) and control group (n=52). The patients of control group were used conventional care while the patients of experimental group received the psychological behavior therapy, relaxation therapy and music therapy within 72 hours after surgery for evaluation of postoperative pain and anxiety on the basis of conventional nursing, and we carried on the comparison of two groups of patients with postoperative hospital stay. Results Two groups of patients with SAI and TAI were no statistical difference at time of admitting to hospital (P>0. 05) ; the SAI and TAI of the experimental group patients in postoperative one day and three days after operation are lower than those of the control group, and the difference between the two groups after surgery was statistically significant (P<0. 05);the time effect on the result without statistical significance (P>0. 05);NRS score of different time points after surgery within two groups showed that the experimental group NRS scores were lower than those of the control group, and the differences were statistically significant between two groups (P<0. 05); the time effect on the result without statistical significance (P >0. 05); the experimental group patients in hospitalization days and postoperative hospitalization days were shorter than these of the control group, the difference between the two groups have statistical significance (P <0. 05). Conclusions Relaxation therapy and music therapy can significantly improve the patient′s perioperative anxiety, reduce the pain after surgery. For gastrointestinal cancer patients perioperative health of body and mind and postoperative rehabilitation, it has a positive role.