Bronchiolitis ; epidemiology ; virology ; Case-Control Studies ; Child, Preschool ; China ; epidemiology ; DNA, Viral ; Female ; Human bocavirus ; isolation & purification ; Humans ; Infant ; Male ; Metapneumovirus ; isolation & purification

Objective To analyze relationship between elderly patients with acute cerebral infarction hyponatremia and their nutrition status.Methods One hundred and twenty patients were selected who hospitalized from January 2011 to December 2012.All the patiens' fasting venous blood samples were taken in the next day morning after hospitalied.and then performed the routine blood test,blood biochemical testing items,observed the level of serum sodium (Na),hemoglobin (Hb),total protein (TP),serum albumin (ALB),prealbumin (PA),C-reactive protein (CRP) and lymphatic cell counts (LY) were measured.According to the value of serum sodium,all patients were classified to lower sodium group (serum sodium ＜ 135 mmol/L,36cases),normal group (serum sodium range from 136 to 145 mmol/L,76 cases),and higher sodium group (serum sodium ＞ 145 mmol/L,8 cases).The results were statistically analyzed.Results The incidence of hyponatremia was 30％ (36/120).The levels of TP,ALB,PA,Hb and LY in lower sodium group were (50.35 ± 8.61) g/L,(28.35 ± 6.98) g/L,(89.96 ± 12.13) mg/L,(94.13 ± 25.36) g/L and (0.87 ±0.51) × 109 respectively,lower than that of normal group ((65.30 ± 5.48) g/L,(37.50 ± 3.63) g/L,(178.14 ± 18.61) mg/L,(124.87 ± 29.08) g/L,(1.67 ± 0.98) × 109 respectively,t =5.0897,7.1058,4.3216,3.8174,5.4237 respectively,P ＜ 0.01),but the level of CRP ((76.55 ± 49.95) mg/L) in lower sodium group was obviously higher than the normal serum sodium group ((21.33 ±35.04) mg/L,t =0.1287,P ＜ 0.01).The level of the serum sodium in ICU was related to the TP,ALB,PA,Hb and CRP(r =0.3176,0.4369,0.3695,0.2408,0.3612,0.0753 respectively,P ＜0.05).Conclusion The level of the merger of hyponatremia was correlated with malnutrition in the elderly acute cerebral infarction patients in ICU.We must strengthen the nutrition support treatment while correcting hyponatremia,in order to improve the clinical curative effect.

Objective To evaluate the efficacy and safety of traditional Chinese medicine in the treatment of skin rash caused by epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI).Methods The clinical randomized controlled trials of traditional Chinese medicine in the treatment of EGFR-TKI-induced skin rash which published in domestic journals were selected.The quality assessment of included literature was made by Jadad score,RevMan 5.2 software was used to make Meta-analysis.Results A total of 6 studies met the inclusion criteria,the 6 studies included 248 patients,133 cases in the treatment group,115 cases in the control group.All patients were 30 to 75 years old.Compared with the control group,the combined OR value of the clinical efficacy of traditional Chinese medicine in the treatment of EGFR-TKIinduced rash was 7.51,with 95 ％ confidence interval 4.46-12.65.Conclusions Meta-analysis shows that the clinical efficacy of traditional Chinese medicine in the treatment of EGFR-TKI-induced skin rash is better than Western medicine,and no adverse reactions.It can be widely used in clinical practice.

Objective To detect the expression of Per2 and VEGF in NSCLC tissue,and analyze its clinical significance.Methods The expression of Per2 and VEGF was measured in 60 NSCLC and 20 normal lung tissue by immunohistochemistry assay,then the relation with clinicopathological parameters was analyzed.Results The positive expression rate of Per2 in NSCLC and in normal lung tissue were 71.7％ and 95.0％ ( x2 =4.683,P ＜0.05 ),while the positive expression rate of VEGF were 58.3％ and 15％ (x2 =11.295,P ＜ 0.01 ).The negative expression of Per2 in NSCLC was correlated with pathological differentiation or TNM stage( x2 =4.413,6.179,all P ＜0.05),while the positive expression of VEGF in NSCLC was correlated with pathological differentiation or lymphatic metastasis (x2 =6.524,P ＜ 0.05 ).The expression of Per2 was negatively correlated with the expression of VEGF in NSCLC (r =-0.381,P ＜ 0.01 ).Conclusion The overexpression of VEGF in NSCLC may contribute to invasion and metastasis,while Per2 may be a beneficial prognosis factor in patients with NSCLC.

Objective To investigate the application data of 6-minute walk test to evaluate the surgical effect of congenital heart disease with pulmonary hypertension.Methods 83 cases of congenital heart disease with pulmonary hypertension were selected and divided into mild(28 patients) ,moderate(30 patients) and severe(25 patients)three groups according to the ultrasound preoperative pulmonary artery systolic pressure estimation (SPAP), all patients underwent surgery correction of heart defects, and before and after surgery the patients were detected SPAP and 6MWT,6MWT observed for evaluating the effect of surgery. Results After surgery,the SPAP of, mild, moderate and severe group were ( 28.7 ± 5. 6) mm Hg, ( 28.7 ± 5.6 ) mm Hg, ( 28.7 ± 5.6 )mm Hg, significantly decreased, 6 MWT were (436. 5 ± 37. 0) m, ( 411.0 ± 39. 8 ) m, ( 338.7 ± 32. 4 ) m, significantly increased, more significant changes in moderate group; The mechanical ventilation and ICU stay of severe group were ( 12. 6 ± 3.2) h, ( 5.5 ± 1.0) d, significandy longer than the mild and moderate groups ( t = 11. 770,6. 118,18. 721,9. 970, all P ＜ 0. 05 ). Conclusion 6-minute walk test to evaluate the surgical effect of congenital heart disease with pulmonary hypertension had clinical significance,and in moderate to severe pulmonary hypertension in patients with clinical assessment more meaningful,and it was worthy of attention.

Objective To explore how to effectively prevent and detect cancer early postoperative intestinal obstruction and treatment methods and means.Methods This retrospective analysis of 13 years occurred in 57 patients with rectal cancer diagnosis and treatment of cases of early postoperative intestinal obstruction process, including fasting,gastrointestinal decompression,fluid therapy,liquid paraffin,diatrizoate and surgical treatment of oral contrast.Results In 57 cases,paralytic intestinal obstruction in 7 cases,conservative treatment was relieved,the success rate was 100.0%;early inflammatory intestinal obstruction in 37 cases after conservative treatment of 36 cases of remission,the success rate was 97.3%;mechanical intestinal obstruction in 13 cases after conservative treatment could not be alleviated,conservative success rate was 0.0% and was cured by reoperation.The first two groups of patients compared with mechanical intestinal obstruction patients,conservative success rate had statistical differences (χ2 =40.08,11.08,all P<0.01).Conclusion Cancer treatment lies in early postoperative intestinal obstruction effective prevention,early detection,close observation and timely and effective treatment,a good grasp of timing of surgery and surgical indications.

By a orthogonal experiment, the influence of different ratio of phosphorus and potassium fertilizers on imperatorin, isoimperatorin and psoralen contents and yield of Glehnia littoralis were studied. The results showed that root dry weight and the yield of G. littoralis increased when reasonably applied phosphorus fertilizer combined with potassium fertilizer within a certain range. And the influence of phosphorus fertilizer was greater than that of potassium fertilizer. The optimal value of root dry weight and yield achieved at both P2O5 360 kg x hm(-2), K2O 270 kg x hm(-2) and P2O5 360 kg x hm(-2), K2O 180 kg x hm(-2). The effects of different phosphorus and potassium treatments on the content of imperatorin, isoimperatorin and psoralen in G. littoralis were determined, which shows that the content increased with the moderate increase of phosphorus and potassium. And the effects of phosphorus fertilizer were more significantly. The isoimperatorin content achieved the largest value at P2O5 360 kg x hm(-2), K2O 270 kg x hm(-2), also a larger content of imperatorin and psoralen. The imperatorin content is the largest when applied P2O5 360 kg x hm(-2), K2O 180 kg x hm(-2), and the isoimperatorin content was higher as well. So that the treatment of P2O5 360 kg x hm(-2), K2O 180 kg x hm(-2) are suitable for promote to the agricultural production, which could improve the quality and yield of G. littoralis.
Agriculture ; Apiaceae ; chemistry ; growth & development ; metabolism ; Coumarins ; analysis ; metabolism ; Drugs, Chinese Herbal ; analysis ; metabolism ; Fertilizers ; analysis ; Phosphorus ; analysis ; metabolism ; Potassium ; analysis ; metabolism

OBJECTIVE: To investigate whether the acetaldehyde dehydrogenase 2 specific activator, Alda-1, can alleviate brain injury after cardiopulmonary resuscitation (CPR) by inhibiting cell ferroptosis mediated by acyl-CoA synthetase long-chain family member 4/glutathione peroxidase 4 (ACSL4/GPx4) pathway in swine. METHODS: Twenty-two conventional healthy male white swine were divided into Sham group (n = 6), CPR model group (n = 8), and Alda-1 intervention group (CPR+Alda-1 group, n = 8) using a random number table. The swine model of CPR was reproduced by 8 minutes of cardiac arrest induced by ventricular fibrillation through electrical stimulation in the right ventricle followed by 8 minutes of CPR. The Sham group only experienced general preparation. A dose of 0.88 mg/kg of Alda-1 was intravenously injected at 5 minutes after resuscitation in the CPR+Alda-1 group. The same volume of saline was infused in the Sham and CPR model groups. Blood samples were collected from the femoral vein before modeling and 1, 2, 4, 24 hours after resuscitation, and the serum levels of neuron specific enolase (NSE) and S100 β protein were determined by enzyme-linked immunosorbent assay (ELISA). At 24 hours after resuscitation, the status of neurologic function was evaluated by neurological deficit score (NDS). Thereafter, the animals were sacrificed, and brain cortex was harvested to measure iron deposition by Prussian blue staining, malondialdehyde (MDA) and glutathione (GSH) contents by colorimetry, and ACSL4 and GPx4 protein expressions by Western blotting. RESULTS: Compared with the Sham group, the serum levels of NSE and S100β after resuscitation were gradually increased over time, and the NDS score was significantly increased, brain cortical iron deposition and MDA content were significantly increased, GSH content and GPx4 protein expression in brain cortical were significantly decreased, and ACSL4 protein expression was significantly increased at 24 hours after resuscitation in the CPR model and CPR+Alda-1 groups, which indicated that cell ferroptosis occurred in the brain cortex, and the ACSL4/GPx4 pathway participated in this process of cell ferroptosis. Compared with the CPR model group, the serum levels of NSE and S100 β starting 2 hours after resuscitation were significantly decreased in the CPR+Alda-1 group [NSE (μg/L): 24.1±2.4 vs. 28.2±2.1, S100 β (ng/L): 2 279±169 vs. 2 620±241, both P < 0.05]; at 24 hours after resuscitation, the NDS score and brain cortical iron deposition and MDA content were significantly decreased [NDS score: 120±44 vs. 207±68, iron deposition: (2.61±0.36)% vs. (6.31±1.66)%, MDA (μmol/g): 2.93±0.30 vs. 3.68±0.29, all P < 0.05], brain cortical GSH content and GPx4 expression in brain cortical was significantly increased [GSH (mg/g): 4.59±0.63 vs. 3.51±0.56, GPx4 protein (GPx4/GAPDH): 0.54±0.14 vs. 0.21±0.08, both P < 0.05], and ACSL4 protein expression was significantly decreased (ACSL4/GAPDH: 0.46±0.08 vs. 0.85±0.13, P < 0.05), which indicated that Alda-1 might alleviate brain cortical cell ferroptosis through regulating ACSL4/GPx4 pathway. CONCLUSIONS Alda-1 can reduce brain injury after CPR in swine, which may be related to the inhibition of ACSL4/GPx4 pathway mediated ferroptosis.
Male ; Animals ; Swine ; Phospholipid Hydroperoxide Glutathione Peroxidase ; Ferroptosis ; Brain Injuries ; Glutathione ; Cardiopulmonary Resuscitation ; Ligases ; Iron

Objective To investigate the clinical value of enhanced recovery after surgery (ERAS) in patients undergoing postoperative early enteral nutrition (EEN) with radical resection for hilar cholangiocarcinoma.Methods The clinical data of 48 patients with hilar cholangiocarcinoma who were admitted to the Drum Tower Clinical Medical College of Nanjing Medical University from July 2006 to September 2014 were retrospectively analyzed.All the 48 patients underwent radical resection for hilar cholangiocarcinoma,including 24 patients receiving postoperative EEN (EEN group) and 24 patients receiving total parenteral nutrition (TPN group).The serologic indices and liver function were detected regularly after operation.Ten percent of albumin (Alb) 10 g was administered by intravenous infusion when Alb ＜ 30 g/L.The indexes of all the 48 patients were compared in the 2 groups at postoperative day 3 and 7,including the serologic indices and liver function,the exhaust time,the volume of Alb infusion,the complications (incisional infection,abdominal infection,pleural effusion,peritoneal effusion and bile leakage) and the duration of hospital stay.The patients were followed up by outpatient examination and telephone interview till September 2014.The measurement data with normal distribution were presented as x ± s,comparison between groups and count data were analyzed using the t test and chi-squared test,respectively.Results Patients in the 2 groups were cured successfully and discharged,and no patient died perioperatively.Patients in the EEN group had a good tolerance for EEN and no occurrence of EEN-related complications was detected.The level of the GGT was (108 ± 73) U/L in the EEN group,which was significantly lower than (225 ± 121) U/L in the TPN group at postoperative day 3 (t =4.041,P ＜ 0.05).The level of the GGT was (142 ± 86) U/L in the EEN group,which was no significantly different from (183 ± 107)U/L in the TPN group at postoperative day 7 (t =1.477,P ＞ 0.05).The postoperative time to anal exsufflation and the duration of hospital stay were (73 ± 18) hours and (15 ± 4) days in the EEN group,which were significantly different from (97 ± 21) hours and (18 ± 4) days in the TPN group,and the volume of Alb infusion was (44 ± 29)g in the EEN group,which was significantly lower than (101 ± 92) g in the TPN group (t =4.295,2.615,2.916,P ＜ 0.05).All the 48 patients were followed up for 1 to 71 months (mediantime,10 months),no patients received reoperation or re-admitted to the hospital due to complications.Conclusion The application of postoperative EEN in enhanced recovery of patients undergoing radical resection for hilar cholangiocarcinoma is safe and effective,it could accelerate the recovery of enteral function,shorten the postoperative duration of hospital stay and reduce the supplement of extrinsic Alb,which is helpful for the fast recovery of patients.

In this study,70 cows with (34.55 ± 8.44) kg per day milk,(2.24 ± 1.01) years at (60-90)d postpartum were chosen from an intensive dairy farm in Heilongjiang province.Ten plasma parameters including reproductive hormones,energy metabolism,liver function and minerals were measured at(60-90)d postpartum from the estrous and ovarian cyst dairy cows,combined with binary Pearson correlation analysis,Logistic analysis and ROC analysis.The results showed that:the incidence of ovarian cysts in this farm was 9.8％,its main reason is negative energy balance;the plasma concentrations of FSH,LH and P4 in ovarian cysts were lower than those in estrus group,but E2 (6.2 ng/L) was significantly higher than that in estrus group ((91.97± 10.62) ng/L);the plasma concentrations of NEFA,BHBA and AST were significantly increased in ovaries cysts at 14-21 d and 60-90 d,and Glu was significantly lower than that in estrus group.When the plasma concentration of BHBA were more than 0.855 mmol/L,NEFA more than 0.585 mmol/L,AST more than 77.0 U/L at 14-21 d postpartum,the risk of ovarian cyst increased.Negative energy balance can cause disorder of reproductive hormone secretion in early lactation cows,and then provoke cyst of follicle.Plasma NEFA,BHBA and AST of dairy cows postpartum can be used to predict the risk of ovarian cysts.