Objective To study the producing method for Euphorbia pekinensis promoted by endophytic fungi.Methods The tissue culture plantlets were innoculated with endophytic fungi,then it was transplanted to the pods and cultured for one year.The biomass and content of terpenoids(isoeuphpekinensin and euphol)were determined.Results The growth of the E.pekinensis was promoted by endophytic fungi E4(Fusarium sp.Ⅰ)and E5(Fusarium sp.Ⅱ).The fresh weight of the root increased 49.45% and 59.74% for E4 and E5 treated,respectively.The plants dry weight coefficient increased 2.99% and 6.48% for E4 and E5 treated,respectively.The contents of isoeuphpekinensin and euphol in the plants were increased for tissue cultured in both bottles and pods.For the plants cultured in pods for one year,the isoeuphpekinensin and euphol increased 92.79% and 40%,respectively treated by E4.The isoeuphpekinensin and euphol increassed 105.32% and 241.38%,respectively treated by E5.Conclusion The endophytic fungi E4 and E5 could promote the growth of E.pekinensis and accumulated terpenoids.

Objective To investigate the effect of heat stress on organ indices, intestinal morphology, gastric mu-cosal HSP70 mRNA expression and glucose metabolic hormones in mice.Methods A single-factor experiment was de-signed for the present research.Eighteen KM mice of the similar age and weight were randomly divided into control group and heat stress group.The weight of the heart, liver, spleen, lung and kidney, as well as the expression of HSP70 mRNA in the mouse gastric mucosa were measured.The plasma concentration of insulin and glucagon, the villus height and crypt depth of the duodenum and jejunum were detected.The histological changes of the liver, duodenum and jejunum were also examined.Results There was no effect of heat stress on the organ indices.It significantly increased the expression of HSP70 mRNA in the gastric mucosa, reduced the plasma insulin level and caused serious injury to the liver, duodenum and jejunum in the mice.Conclusions Heat stress does not significantly affect the organ indices in mice, but can significantly increase the expression of HSP70 mRNA in the gastric mucosa, cause apparent damages in the liver, duodenum and jeju-num, reduce the villus height, crypt depth and villus height/crypt depth ratio of the duodenum and jejunum, and also de-crease the blood insulin concentration in the mice.

Objective To investigate the expression of thymidylate synthase (TS),and the correlations with the clinicopathologcal factors and the prognosis of postoperative fluorouracil chemo-therapy in gastric cancer.Methods 42 patients with gastric cancer who underwent radical gastrectomy were collected from April 2011 to April 2014 in Lu'an Hospital of Anhui Medical University,and the chemotherapy regimen was Cisplatin + Capecitabine or Cisplatin + Teggio.The levels of TS in tumor tissues of 42 patients with gastric cancer were detected by immunohistochemical method.The relationships between TS expression level,clinical features and the prognosis of patients treated with fluorouracil chemotherapy were analyzed.Results In 42 patients with gastric cancer,19 patients (45.2％) have TS high expressions,23 patients (54.8％) have TS low expressions.There were no significant correlations between the expression of TS and age,sex,clinical stage,differentiation degree,tumor location,depth of invasion,lymph node metastasis,vascular tumor thrombus and nerve bundle invasion (all P ＞0.05).There were significant differences between low TS expression and high TS expression in median progression-free survival and 3-year survival rate of patients [35 months vs.17 months,73.9％ (17/23) vs.42.1％ (8/19)],and the differences were statistically significant (x2 =4.788,P =0.029;x2 =4.369,P =0.037).In patients with stage Ⅲ gastric cancer,the median progression-free survival of patients with low TS expression was significantly prolonged compared to that with high TS expression (33 months vs.9 months),and the difference was statistically significant (x2 =4.731,P =0.030).Conclusion TS expression levels have no significant correlations with the clinicopathologcal factors,but closely relate to the prognosis of gastric cancer patients treatment with fluorouracil chemotherapy.The chemotherapy prognosis of TS high expression is worse than that of TS low expression in gastric cancer.

【Objective】 To explore the effects of transurethral blue laser vaporization of prostate for benign prostatic hyperplasia (BPH) on the sexual function and lower urinary tract symptoms. 【Methods】 A retrospective analysis was conducted on 91 BPH patients admitted to our department during Mar. and Aug. 2023. The patients were divided into two groups, 46 in the blue laser group, and 45 in the transurethral plasmakinetic resection of prostate (PKRP) group. The operation time, bladder irrigation time, catheter indwelling time and postoperative hospitalization time of the two groups were compared. Postvoid residual (PVR), maximum urinary flow rate (Qmax), international prostatic symptoms score (IPSS), quality of life score (QoL), international index of erectile function-5 (IIEF-5) score, the Chinese index of premature ejaculation (CIPE) score and incidence of retrograde ejaculation were compared before and 6 months after operation. 【Results】 The operation time \[(24.66±5.38) min vs. (62.50±3.85) min\], bladder irrigation time \[(20.40±2.78) h vs. (51.93±5.02) h\], catheter indwelling time (1.65±0.89) d vs. (6.73±2.98) d\] and postoperative hospitalization time \[(4.26±1.57) d vs. (8.45±2.28) d\] were significantly shorter in the blue laser group than in the PKRP group (P<0.05). There were no significant differences in Qmax, PVR, QoL and IPSS between the two groups before and 6 months after operation (P>0.05). Six months after surgery, the IIEF-5 score and CIPE score of the blue laser group were higher than those of the PKRP group \[(20.02±2.63) vs. (17.64±1.75); (30.88±3.45) vs. (26.25±3.51)\], but the incidence of retrograde ejaculation was lower (4.35% vs. 35.60%), with statistical significance (P<0.05). 【Conclusion】 Blue laser vaporization of the prostate can improve lower urinary tract symptoms and protect sexual function.

A microbial fuel cell (MFC)-based microbial electrochemical sensor was developed for real-time on-line monitoring of heavy metals in water environment. The microbial electrochemical sensor was constructed with staggered flow distribution method to optimize the parameters such as external resistance value and external circulation rate. The inhibition of concentration of simulated heavy metal wastewater on voltage under optimal parameters was analyzed. The results showed that the best performance of MFC electrochemical sensor was achieved when the external resistance value was 130 Ω and the external circulation rate was 1.0 mL/min. In this case, the microbial electrochemical sensors were responsive to 1-10 mg/L Cu²⁺, 0.25-1.25 mg/L Cd²⁺, 0.25-1.25 mg/L Cr⁶⁺ and 0.25-1.00 mg/L Hg²⁺ within 60 minutes. The maximum rejection rates of the output voltage were 92.95%, 73.11%, 82.76% and 75.80%, respectively, and the linear correlation coefficients were all greater than 0.95. In addition, the microbial electrochemical sensor showed a good biological reproducibility. The good performance for detecting heavy metals by the newly developed microbial electrochemical sensor may facilitate the real-time on-line monitoring of heavy metals in water environment.
Bioelectric Energy Sources ; Electrodes ; Metals, Heavy/analysis* ; Reproducibility of Results ; Waste Water ; Water