AIM: To investigate the mechanisms of the anti-myocardial ischemia of Sini decoction at the level of proteome. METHODS: The total proteins of left ventricular muscle were separated with two-dimensional electrophoresis. The 2-DE images were analyzed with the PDQuest7 1 1 software package. The peptide mass fingerprinting(PMF) was made with MALDI-TOF-MS, then the identification and function of the protein was made via bioinformatics. RESULTS: Sini decoction could regulate the expression of 13 proteins in the ischemic myocardium. The proteins could allocated into five groups: energy metabolism-associated proteins, signal-associated proteins ,self-repair-associated proteins, anti-free radical injury-associated proteins and myocardial contractile-associated proteins. CONCLUSION: Sini decoction could prevent the myocardium from ischemia via regulating expression of different proteins.

Objective To investigate the applicability of the K~+ concentration in aqueous humor to estimate postmortem interval(PMI).Methods 30 White New-Zealand rabbit were sacrificed by air embolism and divided into 10 groups.Aqueous humor and vitreous humor were sampled at 0.5h,1h,3h,6h,8h,12h,16h,20h,and 24h after death.The concentration of potassium,sodium and calcium were analyzed by an autoanalyzer and the data were statistically processed by SPSS software for Windows.Results Increase in potassiam concentration in aqueous humor was correlated with the postmortem interval(R~2=0.956).Conclusion Measurement of potassium concentration in aqueous humor may be used for PMI estimation.

Objective To find the mutation of disease-causing genes of sudden unexplained death syn-drome (SU D S ) in the young by whole exome sequencing in one case. Methods O ne SU D S case was found no obvious fatal pathological changes after conventional autopsy and pathological examination. The whole exome sequencing was performed with the Ion Torrent PGMTM Systemwith hg19 as reference se-quence for sequencing data. The functions of mutations were analyzed by PhyloP, PolyPhen2 and SIFT. A three-step bioinformatics filtering procedure was carried out to identify possible significative single nu-cleotide variation (SN V ), which was missense mutation with allele frequency <1% of myocardial cell. Results Four rare suspicious pathogenic SN V were identified. C ombined with the analysis of convention-al autopsy and pathological examination, the mutation MYOM 2 (8_2054058_G/A ) was assessed as high-risk deleterious mutation by PolyPhen2 and SIFT, respectively. Conclusion Based on the second genera-tion sequencing technology, analysis of whole exome sequencing can be a newmethod for the death cause investigation of SU D S. The gene MYOM2 is a newcandidate SU D S pathogenic gene for mecha-nismresearch.

Objective To investigate the expression of tumor necrosis factor alpha(TNF-α)and its relationship with infiltrating lymphocytes in lichen planus(LP).Methods Tissue specimens were obtained from the lesions of 60 patients with LP and normal skin of 20 human controls.Immunohistochemical SP method was used to detect the expression of TNF-α,and infiltrating lymphocytes were counted in TNF-α-positive tissue sections.Results TNF-α was expressed in 72％ of the LP specimens but in none of the control specimens(P ＜ 0.01).Positive staining for TNF-α was mainly located in the membrane of prickle cells,cytoplasm or membrane of dermal infiltrating lymphocytes.The expression of TNF-α in LP was uncorrelated with age,sex or disease course(all P ＞ 0.05),but was positively correlated with infiltrating lymphocyte number (rs =0.47,P ＜ 0.01).Conclusion TNF-α seems to play a certain role in the pathogenesis of LP.

【Objective】 To explore the effects of transurethral blue laser vaporization of prostate for benign prostatic hyperplasia (BPH) on the sexual function and lower urinary tract symptoms. 【Methods】 A retrospective analysis was conducted on 91 BPH patients admitted to our department during Mar. and Aug. 2023. The patients were divided into two groups, 46 in the blue laser group, and 45 in the transurethral plasmakinetic resection of prostate (PKRP) group. The operation time, bladder irrigation time, catheter indwelling time and postoperative hospitalization time of the two groups were compared. Postvoid residual (PVR), maximum urinary flow rate (Qmax), international prostatic symptoms score (IPSS), quality of life score (QoL), international index of erectile function-5 (IIEF-5) score, the Chinese index of premature ejaculation (CIPE) score and incidence of retrograde ejaculation were compared before and 6 months after operation. 【Results】 The operation time \[(24.66±5.38) min vs. (62.50±3.85) min\], bladder irrigation time \[(20.40±2.78) h vs. (51.93±5.02) h\], catheter indwelling time (1.65±0.89) d vs. (6.73±2.98) d\] and postoperative hospitalization time \[(4.26±1.57) d vs. (8.45±2.28) d\] were significantly shorter in the blue laser group than in the PKRP group (P<0.05). There were no significant differences in Qmax, PVR, QoL and IPSS between the two groups before and 6 months after operation (P>0.05). Six months after surgery, the IIEF-5 score and CIPE score of the blue laser group were higher than those of the PKRP group \[(20.02±2.63) vs. (17.64±1.75); (30.88±3.45) vs. (26.25±3.51)\], but the incidence of retrograde ejaculation was lower (4.35% vs. 35.60%), with statistical significance (P<0.05). 【Conclusion】 Blue laser vaporization of the prostate can improve lower urinary tract symptoms and protect sexual function.