The stability of the authors' self-formulated Ftorafur liposome was studied by the classical isothermal method at 60, 70 & 80℃. The particle size distribution of the particulates and the rate of their coalescence were determined by using the Coulter counter. It was shown that the change of particle size of Ftorafur liposome was a first-order reaction. The rate equation and the activation energy of coalescence and the kinetic constant of coalescence at room temperature were derived. The particle size distribution, appearance and homogeneity changed just slightly after storage at room temperature and in refrigerator (5℃) for 30 months, respectively. Drug enclosed within liposome leaked out about 3.8 % after storage for 3 months at 37℃ (?0.1℃). The above results indicate that the Ftorafur liposome is rather stable.

BACKGROUND:Nowadays treatment of osteoarthritis with drugs is not ideal. In recent years, more and more scientists try to use stem cells to treat osteoarthritis.
OBJECTIVE:To explore the stem celltreatment for osteoarthritis in order to promote its clinical application and find out its difficulties.
METHODS:A computer-based online search of PubMed database and CNKI database between March 1998 and October 2013 was performed to search related articles with the key words of“stem cell, osteoarthritis, bone metabolism, bone marrow mesenchymal stem cells”in English or in Chinese, respectively. The word“AND”was used for the connection between the word retrieval. Literatures related to stem cells treatment for osteoarthritis were selected;in the same field, the articles published lately in authoritative journals were preferred.
RESULTS AND CONCLUSION:A total of 79 literatures were primarily selected, and 50 documents were involved for summary according to inclusion criteria. The stem celltreatment for osteoarthritis is realized mainly by promoting the repair of cartilage tissue. Commonly used methods are stem celltransplantation and induced differentiation of stem cells. Stem celltreatment has broad application prospects for the treatment of osteoarthritis.

Objective To investigate the method of handmade microwire lasso device for the management of coil migration during intracranial aneurysm embolization. Methods Two migration coils were removed from intracranial arteries using handmade microwire lasso rings. The first coil prolapsed completely from an aneurysm sac and flowed to the middle cerebral artery M2 segment. The second coil partially prolapsed out into a parent artery. The microwire lasso device was made of 4-0 silk thread and fixed using 0. 36 mm (0. 014 inch)microwire and 0. 43 mm (0. 017 inch)microcatheter. Results After removing 2 migration coils from intracranial arteries,angiography did not reveal vascular injury and thrombosis. There was no aneurysm rupture. After awaking from anesthesia,the patient did not have neurological deficit. The patient was followed up for 3 months after procedure. MR angiography confirmed the patency of related arteries. Conclusion The handmade microwire lasso device for the management of coil migration in the process of aneurysm embolization is a simple,effective,and economical method.

Objective: To study the pharmacokinetics of fluoroful temperature-sensitive liposomes in rabbits. Methods: Fluoroful temperature-sensitive liposomes were intravenously administered into rabbits. Livers were heated to (42?0.1)℃ by microwave, blood concentrations of fluoroful were determined, the pharmacokinetics parameters of it were calculated. Results: After being heated, the drug releasing of fluoroful temperature-sensitive liposomes was accelerated, and the drug were controlled to be released into liver. Conclusion: Fluoroful temperature-sensitive liposomes can be controlled to release specifically into livers by heating the organs.

Animals ; Apoptosis ; genetics ; immunology ; Apoptosis Regulatory Proteins ; genetics ; Autoimmune Lymphoproliferative Syndrome ; genetics ; immunology ; physiopathology ; Humans ; Mutation ; T-Lymphocytes ; immunology

Objective To analyze stenting and dual catheter technique in protection of posterior inferior cerebellar artery and its imaging and clinical follow-up results in the endovascular treatment of vertebral artery dissecting aneurysms. Methods From January 2012 to December 2014,the clinical data of 4 patients with vertebral artery dissecting aneurysm involving posterior inferior cerebellar artery treated with endovascular method and admitted to the Department of Neurosurgery,Xuanwu Hospital,Capital Medical University were enrolled retrospectively. The whole brain DSA examinations were performed at 6 months after procedure. The clinical follow-up period ranged from 12 to 24 months. Results Three of the 4 patients were treated with posterior inferior cerebellar artery-vertebral artery stenting,one patient was treated by using bidirectional dual catheter technique to protect posterior inferior cerebellar artery. The interventional therapy of 4 patients were all successful. Immediately after procedure,angiography revealed that the posterior inferior cerebellar arteries were patent,no ischemic symptoms were observed. The angiography at 6 months after procedure revealed no recurrence of aneurysms;the posterior inferior cerebellar arteries were patent without in-stent stenosis. No new symptoms of neurological deficit were observed during 12 to 24-month follow-up. Conclusion In the interventional treatment of vertebral artery dissecting aneurysms of posterior inferior cerebellar artery,using the posterior inferior cerebellar artery-vertebral artery stenting technique and bidirectional dual catheter technique may safely and effectively protect the posterior inferior cerebellar artery.

Benzene ; adverse effects ; Hearing ; Humans ; Noise, Occupational ; adverse effects

Objective To observe the effect of immunity condition and clinical manifestations of children with recurrent oral-ulcer(ROU)treated with aciclovir(ACV) and a-polyresistin.Methods Forty-eight patients with ROU were randomly divided into whole treated group(25 cases)and partial treatment group(23 cases).The changes of their peripheral blood lymphocyte subgroups,before and after treatment,were measured and contrasted to analyze the relationship with curative effect.Results The ratios of CD3,CD4,and CD4/CD8,after the treatment,increased obviously,while CD8 lowered obviously in treatment group(P0.05).The total clinical efficacy rate was 47.82%,moreover the patients were recurrent after 1 year.Conclusions ACV and a-polyresistin,through antivirus and regulating the immunity function get the 96% total clinical efficacy rate,and that controle the recurrent rate.It is superior to the traditional method.

Triptolide(TP)is a diterpenoid epoxide purified from a Chinese herb Tripterygiumwilfordii Hook. f.. lt has been shown to have multiple pharmacological activities,such as anti-tumor,anti-inflammatory,immune-suppressive effects. TP can reduce airway hyperresponsiveness,decrease the proliferation of airway smooth muscle,and produce potential therapeutic effect in airway remodeling. The effect of TP on allergic asthma may be related to its inhibition of maturation of dendritic cells,rebalance of the function of Th1 and Th2,reduction of the secretion of inflammatory factors,and decrease of the infiltration of eosinophils.

Objective To evaluate the diagnosis and therapy of intestinal obstruction caused by multiple jejunal diverticula.Methods Clinical data of intestinal obstruction caused by multiple jejunal diverticula in 18 cases admitted from Jan 2001 to Aug 2012 were analyzed retrospectively.Results All patients experienced the symptoms of abdominal pain and abdominal distension,5 cases experienced nausea and vomit,2 cases had a fever.Plain abdominal X-ray series demonstrated distension of small bowel and multiple air-fluid levels,5 cases with anemia,the average value of hemoglobin was (9.5 ± 1.0) g/L,B-ultrasonography revealed bowel dilatation in 7 out of 15 cases,9 cases underwent abdominal computed tomography and all had positive sign of small bowel distension and multiple air-fluid levels,mesenteric volvulus was suspected in 1 case.All patients underwent laparotomy,the diagnosis of multiple jejunal diverticula were confirmed clinically and by the pathology.In a follow-up ranging from 2 to 25 months,1 case died,the others were symptoms free.Conclusions Multiple jejunal diverticula was a less common disorder,occurring mainly in old man,with a low preoperative definite diagnosis.Resection of affected intestinal segment with primary anastomosis results in satisfactory prognosis.