Objective The pathological changes on SARS CoV infected Microtus brandtii,Lewis rat and Rhesus macaque and the possibility as SARS animal model were studied.Methods The amimals were infected with SARS-CoV through nasal cavity and sacrificed for collecting pathologic tissues after inoculation.The pathological changes,immuno-response and the replication of SARS-CoV in vivo were tested with microscopy,ELISA and virus isolation.Results Similar pathological changes were observed in SARS CoV infected Rhesus macaques,Lewis rats and Microtus brandtii and the pathological changes were similar with those of human.Conclusion All of the tree species of animals infected with SARS-CoV showned similar pathological changes with human SARS case.Among them,SARS-CoV infected Rhesus macaques is the best one in terms of pathological changes.Therefor the infected Rhesus macaque may be one of the most ideal animal model to study the pathogenesis and evaluation of anti-SARS-CoV vaccine.

The outbreaks of Zika virus infection in different regions of the world underscore the potential for the virus to spread further in the Americas and beyond.Zika is a mosquito-borneFlavivirusand is transmitted by Aedes spp. mosquitoes.It is has been reported recently that Zika virus intrauterine infection causes fetal brain abnormality and microcephaly, and WHO encourages pregnant women in countries at risk MUST protecting themselves from mosquito bites. Until now, there is no imported case in China.We have established resource and technology storage to reply to the potentialZika cases in the future, therefore, people do not need to panic in China.

Alzheimer′s disease ( AD) , is a neurodegenerative disorder of the brain that is characterized by loss of memory and cognitive decline.At present, AD etiology remains unclear and there are no effective prevention and treatment measures in clinical practice.Peroxisome proliferator-activated receptorγ( PPARγ) is a ligand-regulated nuclear hormone receptor.Recent studies showed PPARγ-pathway played an important role in the pathogenesis of AD and some PPARγagonists have been proven to be neuroprotective in vitro and in vivo models.This paper reviews the roles of PPARγand related mechanisms in AD, summarizes affecting factors about PPARγpathway.Particularly, the effect of cyanidin-3-O-glucoside ( Cy3G) , one of the anthocyanidin glycoside forms, is a compound of naturally occurring phenolic compounds, suggesting the neuroprotective effect of Cy3G might be used as a potential natural PPARγagonist in the nervous system.

Optogenetics is a technology invented by Boyden in 2005 that allows targeted, fast control of precisely defined events in neural system. It was widely used in Parkinson disease and emotional neural circuit studies. Optogenetics was selected by Nature Methods for method of the year 2010. This paper reviews on the research advances and applications of optogenetics.

Animal model plays an important role in prevention and control of infectious disease, which could link basic research in laboratory with clinical diagnosis and treatment for human patients.Mouse is the most widely used animal model of infectious disease, however, adult immunocompetent mice are resistant to some pathogens.The highly genetically diverse Collaborative Cross (CC) mice could recapitulate many of the genetic characteristics of an outbred population, such as humans.Based on this, this review will focus on the application and research progress of CC mice in infectious disease (including viruses, bacteria, fungi etc.), which could provide useful reference data for expansion of animal model resource bank, and implement of precision medicine of major and new emerging infectious diseases.We hope this review could serve as a modest spur to induce other researchers to come forward with their valuable contributions.

H5N1 subtype of avian influenza virus (AIV) has been prevailing in many countries over the world, which has threaten human health. The establishment of AIV animal models may provide a tool for the study on the infection, mutation of avian influenza virus and pathogenesis of the disease. The pathogenicity of H5N1 to mammalian such as cynomolgus macaques, ferrets, mice, rats, gerbils, cats were summarized in current review, which may provide meaningful help for the establishment and study on ideal experiment animal models.

Dust ; Female ; Humans ; Male ; Occupational Exposure ; adverse effects ; Pneumoconiosis ; diagnostic imaging ; etiology ; Poaceae ; Radiography, Thoracic ; Silicates ; adverse effects

The dynamic characteristics of the area of the atrial septal defect (ASD) were evaluated using the technique of real-time three-dimensional echocardiography (RT 3DE),the potential factors re sponsible for the dynamic characteristics of the area of ASD were observed,and the overall and local volume and functions of the patients with ASD were measured,RT 3DE was performed on the 27 normal controls and 28 patients with ASD.Based on the three-dimensional data workstations,the area of ASD was measured at P wave vertex,R wave vertex,T wave starting point,and T wave terminal point and in the T-P section.The right atrial volume in the same time phase of the cardiac cycle and the motion displacement distance of the tricuspid annulus in the corresponding period were measured.The measured value of the area of ASD was analyzed.The changes in the right atrial volume and the motion displacement distance of the tricuspid annulus in the normal control group and the ASD group were compared.The right ventricular ejection fractions in the normal control group and the ASD group were compared using the RT 3DE long-axis eight-plane (LA 8-plane) method.Real-time three-dimensional volume imaging was performed in the normal control group and ASD group (n=30).The right ventricular inflow tract,outflow tract,cardiac apex muscular trabecula dilatation,end-systolic volume,overall dilatation,end-systolic volume,and appropriate local and overall ejection fractions in both two groups were measured with the four-dimensional right ventricular quantitative analysis method (4D RVQ) and compared.The overall right ventricular volume and the ejection fraction measured by the LA 8-plane method and 4D RVQ were subjected to a related analysis.Dynamic changes occurred to the area of ASD in the cardiac cycle.The rules for dynamic changes in the area of ASD and the rules for changes in the right atrial volume in the cardiac cycle were consistent.The maximum value of the changes in the right atrial volume occurred in the end-systolic period when the peak of the curve appeared.The minimum value of the changes occurred in the end-systolic period and was located at the lowest point of the volume variation curve.The area variation curve for ASD and the motion variation curve for the tricuspid annulus in the cardiac cycle were the same.The displacement of the tricuspid annulus exhibited directionality.The measured values of the area of ASD at P wave vertex,R wave vertex,T wave starting point,T wave terminal point and in the T-P section were properly correlated with the right atrial volume (P＜0.001).The area of ASD and the motion displacement distance of the tricuspid annulus were negatively correlated (P＜0.05).The right atrial volumes in the ASD group in the cardiac cycle in various time phases increased significantly as compared with those in the normal control group (P=0.0001).The motion displacement distance of the tricuspid annulus decreased significantly in the ASD group as compared with that in the normal control group (P=0.043).The right ventricular ejection fraction in the ASD group was lower than that in the normal control group (P=0.032).The ejection fraction of the cardiac apex trabecula of the ASD patients was significantly lower than the ejection fractions of the right ventricular outflow tract and inflow tract and overall ejection fraction.The difference was statistically significant (P=0.005).The right ventricular local and overall dilatation and end-systolic volumes in the ASD group increased significantly as compared with those in the normal control group (P=0.031).The aRVEF and the overall ejection fraction decreased in the ASD group as compared with those in the normal control group (P=0.0005).The dynamic changes in the area of ASD and the motion curves for the right atrial volume and tricuspid annulus have the same dynamic characteristics.RT 3DE can be used to accurately evaluate the local and overall volume and functions of the right ventricle.The local and overall volume loads of the right ventricle in the ASD patients increase significantly as compared with those of the normal people.The right ventricular cardiac apex and the overall systolic function decrease.

Collaborative Cross mice ( CC mice) are series of inbred mice strains generated from hybrid strains of mice with different genetic background which used for human complex diseases and genetic diversity diseases studies. Genetic diversity of CC mice can reflect different mouse subspecies, the single nucleotide polymorphism is four times than traditional inbred mice. CC mice are more and more widely used in the field of life science and medical research. Based on information retrieval of CC mice, we introduced the related information resources of CC mice origin, database, application tools, and research results, to promote CC mice resources to be used widely in China.

Objective The present study is to investigate the effect of EPO pretreatment on TNF-? expression in cultured cardiac myocytes with H/R and to explore the possible NF-?B signal transduction mechanism. Methods The model of cultured cardiac myocytes with H/R was established and the cardiac myocytes were divided into 4 groups, including EPO group (treat with EPO 10?U/ml 24?h before H/R), EPO+PDTC group (treat with EPO 10?U/mL and PDTC 5??g/ml 24?h before H/R), PDTC group (treat with PDTC 5??g /ml 24h before H/R) and control group. Change of TNF-? gene expression before and after H/R in cardiac myocytes was detected with RT-PCR and western blot. Change of NF-?B activity before and after H/R in cardiac myocytes was assayed with EMSA. Results Before H/R, there was no significant difference in TNF-? mRNA and protein expression between the 4 groups and after H/R, TNF-? mRNA and protein expression increased significantly in the 4 groups compared to control group before H/R. After H/R, TNF-? mRNA and protein expression was lower in EPO group than in the other 3 groups. Before H/R, NF-?B activity was higher in EPO group than in the other groups. After H/R, NF-?B activity increased significantly in all the 4 groups compared to the control before H/R and NF-?B activity was lower in EPO group than in the other groups. Conclusion EPO pretreatment inhibited the upregulation of TNF-? gene expression after H/R in cardiac myocytes, which might be related to the inhibition of NF-?B activation; EPO pretreatment might inhibit the activation of NF-?B after H/R through the negative feed-back mechanism of NF-?B activation.