Objective To explore the influence of arsenous acid on hepatic and colonic cancer cell lines BEL-7402 and HT-29 .The effect of bortezomib combined with chemotherapeutic medicine and the optimal combination plan of them were also investigated .Methods We detected cell proliferation by MTT to get the inhi-bition rate of two cell lines and further to determine IC 50 ( inhibitory concentration50%) of 5-Fu,oxaliplatin or arsenous acid;preincubated cancer cells for 2 h、4 h or 8 h by bortezomib .Chemotheraputic medicine were com-bined respectively .The cell proliferation and apoptosis were analysed by MTT ,TUNEL and Annexin V -PI.Re-sult s The 24 h inhibition rate of arsenous acid to BEL -7402 and HT-29 was:0.59 ±0.09、0.71 ±0.12 re-spectively;the IC50 of 5 -Fu,Oxaliplatin or arsenous acid to BEL -7402 was:5.33 ±0.07 mg/L,28.73 ± 0.72 mg/L,25.93 ±4.05 mg/L, while to HT -29 was:7.33 ±1.13 mg/L、53.94 ±1.23 mg/L,21.93 ± 2.05 mg/L.Both inhibition rate and apoptosis rate were enhanced when chemotheraputic medicine was combined with bortezomib .Co nclusion Arsenous acid can inhibit the growth of cancer cells obviously;the susceptibility of each chemotherapy medicine can be strengthened significantly when it is combined with bortezomib ,P<0.05.

Aquaporin-4 (AQP4) is an important structural basis for water regulation and trans- portation in the central nervous system,which participates in physiopathological processes, such as cerebrospinal fluid reabsorption, osmotic regulation, and cerebral edema formation. Protein kinase C (PKC) exists in various cells, it catalyzes the phosphorylation of serine or threonine residues on various protein substrates.In recent years, studies have found that there is a certain correlation between the expressions of AQP4 and PKC after ischemic stroke.This article re- views the distribution,function,functional regulation of AQP4 and PKC,and their relations in ischemia-reperfusion.

Objective To investigate the protective effect of recombinant human growth hormone (rhGH) on gastric mucosa of rats with hemorrhagic shock. Methods Eighteen healthy male Sprague Dawley (SD) rats were randomly assigned into 3 groups, 6 rats for each group: control group, hemorrhagic shock group and rhGH treated group. The animals in control group were subjected to anaesthesia and intubation, without hemorrhagic shock and resuscitation, and all the parameters were determined 4h after intubation. The animals in hemorrhagic shock group and the rhGH treated group were not only given anaesthesia, intubation for carotid artery and internal jugular vein, but also reproduced as hemorrhagic shock-resuscitation model by bleeding from carotid artery and blood transfusion via internal jugular vein. The animals in rhGH treated group were given rhGH (1.5U/kg) when resuscitation began. In the hemorrhagic shock group and rhGH treated group all the parameters were determined 2h after resuscitation. Gastric mucosal blood flow (GMBF) was determined with laser doppler flowmetry (LDF), and the extent of gastric mucosal injury was assessed with optical microscope and transmission electron microscope (TEM). Results GMBF of the rats in hemorrhagic shock group was significantly lower than that in control group (260.4?49.6bpu vs. 418.6?57.3bpu, P0.05). The gastric mucosa injuries in the rats of rhGH treated group were greatly improved. Conclusion The rhGH, through enhancing the GMBF, can ameliorate the gastric mucosal ischemic-reperfusion injury in rats with hemorrhagic shock.

Aged ; Endocarditis ; etiology ; therapy ; Humans ; Kidney Transplantation ; adverse effects ; Male

4 ml, which was 45.1% (23 of 51)(?2=20.7, P4 ml were 3.8% (4 of 105) and 29.4% (15 of 51) respectively, with statistically significant difference between the two groups (?2=21, P68Gy was 81.3% (65 of 80), and group ≤68Gy 56.6% (43 of 76)(?2=11.1, P

Forty eight patients with knee joint lipohemarthrosis underwent X-ray, CT, MRI and ultrasonography examinations, the diagnosis was confirmed by knee operation or puncture.The findings of 4 imaging methods were compared.Plain radiographs displayed fracture lines in 39 cases and fat-liquid layer of suprapatellar bursa in 40 cases, it displayed suprapatellar fat-liquid layer in 5 out of 9 occult fracture cases.CT displayed fracture lines in 48 cases and joint lipohemarthrosis in 48 cases; 3D imaging showed fracture lines better.MRI displayed fracture lines in 45 cases and joints lipohemarthrosis in 48 cases, and also showed the intracapsular structure injury.Ultrasonography revealed fracture lines in 15 cases and joint lipohemarthrosis in 48 cases, lipohemarthrosis was displayed in 7 out of 9 occult fracture cases.All 4 diagnostic imaging methods have advantages and disadvantages for diagnosis of lipohemarthrosis, and should be chosen on the basis of clinical needs.

Object To develop a mathematical model on the relationship between the temperature used in the course of extraction of medicinal herbs and the concentration of active component obtained Methods Based on the kinetic model on medicinal herb extraction process reported in our previous article *, diffusion coefficient and concentration gradient varying with temperature were further studied Results The process which tanshinone was extracted from Salvia miltiorrhiza Bge at different temperatures showed that the model could well match the experimental data Conclusion The relation between the temperature and the concentration of active component could be described by this model

Objective To study the clinical significance of recognizing Zuckerkandl tubercle (ZT) during thyroid surgery. Methods One hundred and sixty-one patients (218 sides) having underwent thyroid lobectomy or thyroidectomy were retrospectively analyzed. The presence of ZT, the position and size of ZT, and the relationship between ZT and recurrent laryngeal nerve (RLN), superior parathyroid (SP) were observed. Results In 218 sides, 179 sides (82.1%) found ZT, with right side in 93 cases and left side in 86 cases. The main relationship between ZT and RLN was A type, accounting for 90.5%(162/179). There were no statistical differences in ZT grade and the relationship type between ZT and RLN between left side and right side (P>0.05). There was negative correlation in ZT grade and the relationship type between ZT and RLN (r=-0.269, P<0.01). In right side, 92.3%(72/78) of SP located on the top of ZT at 10-11 o′clock position;in left side, 94.6%(70/74) of SP located on the top of ZT at 1-2 o′clock position. Conclusions ZT is an important anatomical mark in the thyroid surgery, which helps to identify and protect the RLN and SP, so as to reduce surgical complications.

Objective To investigate the associations of IL-6(rs6949149,rs1800796 and rs10499563) and IL-6R(rs2228145) single nucleotide polymorphisms(SNP) with the risk of gastric cancer in Chinese Han population of Xuyi,Huai'an.Methods A total of 400 patients with gastric cancer and 400 healthy controls were enrolled in this case-control study.The genotypes of the four SNPs were detected by the Mass-Array technology.The associations between the IL-5/IL-6R SNPs and the risk of gastric cancer were analyzed by Logistic regression model.Results There was a significant difference in the distribution of the IL-6 rs1800796 genotypes between the patients with gastric cancer and healthy controls(x2 =6.213,P =0.045).The IL-6 rs1800796 GG genotype was associated with a increased risk of gastric cancer(adjusted OR =1.27,95 ％ CI:1.15-3.20,x2 =6.326,P =0.012).Subgroup analysis showed that the associations between the IL-6 rs1800796 GG genotype and the risk of gastric cancer were obvious in the female(adjusted OR =3.99,95％ CI:1.36-11.71,x2 =6.358,P =0.012) and the patients with H.pylori infection(adjusted OR =2.20,95％ CI:1.08-4.45,x2 =4.770,P =0.029).Conclusion The IL-6 rs1800796 GG geuotype is associated with the risk of gastric cancer,especially in the female and the patients with H.pylori infection.

Objective To explore central lymph node metastasis (CLNM) of cN0 papillary thyroid carcinoma(PTC).Methods We evaluate the clinical data of 245 patients with cN0 unilateral TPC undergoing total thyroidectomy and bilateral central lymph node dissection.Results Central lymph node metastasis of cN0 TPC was 36.8％ (91/245),significantly related to gender(x2 =5.626,P =0.018),age (x2 =6.255,P =0.012),tumor size (x2 =20.063,P ＜ 0.001) and capsule invasion (x2 =15.024,P ＜ 0.001).The central lymph node metastasis was 51.7％ (60/116) in 116 patients with tumor size ＞ 1 cm,and the contralateral CLNM was 22.4％ (26/116) which was related to capsule invasion (x2 =9.737,P =0.002) and delphian LNM(x2 =10.982,P =0.001) There were 6 cases (2.4％) suffering from temporary unilateral recurrent laryngeal nerve injury.96 cases (39.2％) suffered from temporary hypoparathyroidism and 3 cases (1.2％) from permanent hypoparathyroidism.The median follow-up time was 40 months.6 cases were found regional lymph node recurrence on the ipsilateral side.Tumor size ＞ 1 cm was significantly related with recurrence (P =0.008),as CLNM with higher recurrence rate (P =0.001).Conclusions CLNM is common in cN0 PTC patients,and central lymph node metastases increase the risk of recurrence.Prophylactic bilateral central lymph node dissection is suggested for tumor size ＞ 1 cm cases.