The purpose of this study was to investigate the effects of different Ca~(2+) concentrations on the ventricular fibrillation threshold(VFT) in the acute reg- ional ischemic isolated, paced rat hearts and to investigate their relations to the levels of cAMP, cGMP and ATP in the myocardium. It was demonstrated that a positive correlation was observed between [Ca~(2+)] and the percentage of VFT lowered in the ischemic hearts (r=0.7998, P

Protein Z (PZ) is a vitamin K-dependent protein. As a cofactor for the protein Z-dependent protease inhibitor (ZPI), it inhibits coagulation factor X under the existence of phospholipid and calcium ion, and increases the ZPI activity by nearly 1000-fold, thus it plays a role in the process of thrombosis. ZPI inhibits coagulation factor Ⅺ a alone. ZPI activity is also consumed in the process of inhabiting factor Ⅹa and Ⅺa. This article reviews the biological characteristics of PZ and ZPI and their association with stroke.

Objective: To explore the effect of selenium-protein polysaccharide extracted from Qinba (selenium-mushroom)(秦巴硒菇) on apoptosis of K562 cells.Methods: Tumor-bearing mice model was established,and selenium-protein polysaccharide extracted from Qinba selenium-mushroom contained-herb serum was prepared by the method of serum pharmacology.K562 cells were conventionally cultured.Growth inhibition rate of tumor cells was detected by methyl thiazolyl tetrazolium(MTT) method.Morphological changes of apoptotic cell were observed under a fluorescent microscope.Cell apoptosis was observed by DNA electrophoresis.Caspase3 relative gene was measured by colorimetry.Results: Selenium-protein(polysaccharide) extracted from Qinba selenium-mushroom could significantly inhibit the growth of K562 cells,there was a remarkably positive correlation between drug concentration,time and inhibitory rate.Apoptotic phenomenon was certained via morphological examination and DNA electrophoresis.Compared with control group,caspase3 gene was markedly upregulated.Conclusion: Selenium-protein(polysaccharide) extracted from Qinba selenium-mushroom could induce apoptosis of K562 cells.The mechanism may be related to upregulation of caspase3.

OBJECTIVE: To summary the application of new types of nanoparticles carriers emerged in recent years in drug or gene delivery. METHODS: By sorting, analyzing and summarizing domestic and foreign literatures, the characteristics, in vivo and in vitro properties and the applications in pharmacy of novel nanoparticles carriers such as nano cochleates, virus-like particles, hydrogel nanoparticles, gold nanoshells, carbon nanomaterials, quantum dots and dendrimers were reviewed and elaborated. RESULTS AND CONCLUSION: Nanoparticles possess special physical and chemical properties which could improve the stability and bioavailability of drugs and have a targeting and sustained release effect.

Aged ; Endocarditis ; etiology ; therapy ; Humans ; Kidney Transplantation ; adverse effects ; Male

Objective To compare the surgical outcome of Lichtenstein,open preperitoneal technique and double-laver mesh technique in tension free repair of incarcerated inguinal hernia.Methods Totally 438 cases of indirect inguinal hernia in our hospital were retrospectively analyzed from January 2008 to January 2012,io which 154 patients received Lichtenstein,186 patients received open preperitoneal technique and 98 patients received double-layer mesh technique.Results The recurrence rates of these three groups were low,0.65％,0.54％,0,respectively,and there was no significant difference in the recurrence rate between these 3 groups (P ＞0.05),but significant difference in the foreign body sensation and postoperative pain between the open preperitoneal technique group and the other two groups(P ＜ 0.05),and the cost of the double-layer mesh technique group was ahout 2000 yuan higher than the other two groups.Conclusion The open preperitoneal technique can reduce the postoperative pain and foreign body sensation after surgery with low cost,and it is a good choice for inguinal hernia repair.

OBJECTIVE To compare the changing trends of antibiograms of Salmonella typhi and S.paratyphi A isolates.METHODS A total of 42 isolates of Salmonella obtained from blood cultures patients in the First Affiliated Hospital of Chinese Medical University between 2001-2004 were included in the study.K-B test was used for the antimicrobial susceptivity test and the results were read based on National Committee for Clinical Laboratory Standards(NCCLS) of USA.RESULTS Twenty two isolates were identified as S.typhi and 20 were identified as S.paratyphi A.Sensitivity of S.typhi isolates to cephalosporins and chloramphenicol was found to have increased from 2001 to 2004 while that of S.paratyphi A showed a decline,meanwhile there was obviously increasing in resistance to ciprofloxacin.CONCLUSIONS Perhaps the schedule of empirical treatment of typhoid fever needs to be adjusted.

Objective To observe the clinical efficacy of psoriasis treated with avermectin A and astragalus and detect the changes of TNF-a in serum. Preliminarily study the anti-inflammatory and immunomodulatory mechanism of avermectin A and astragalus in the treatment of psoriasis. Methods 20 patients were randomly recruited into a control group (9 cases) and a treatment group (11 cases). Another 20 healthy people were recruited for comparison. The control group was administrated with avermectin A orally, 0.2±0.6 mg/kg·d, with the symptoms turning better, reduced the dosage till the recovery, the treatment group was injected with astragalus injection on the therapeutic basis of the control group. Detect and compare the levels of TNF-α in the serum of the patients with psoriasis before and after the treatment in both groups, then make a contrast to the healthy people. Results 20 patients got clinical cure. The level of TNF-α in the serum of healthy persons is 11.7± 6.7 ng/L, and the level of TNF-α in the serum of psoriasis patients before treatment is (45.4±12.4) ng/L and (46.4±16.9) ng/L in the treatment group and the control group respectively. Compared with healthy control group, the difference is significant. After treatment, is the TNF-αlevels dropped to (13.8± 5.4) ng/L and (20.9±6.3) ng/L in the treatment group and the control group respectively, showing no significant difference compared with healthy control group. The difference between the two group is also significant. Conclusion The efficacy of treating psoriasis with avermectin A and astragalus is better than using avermectin A exclusively. The mechanism may lie in its inhibiting the formation of TNF-α and so thus exerting the anti-inflammatory and immune regulatory functions.

Objective To assess the clinical history and MRI findings of pituitary adenoma of different hormonesecreting type.Methods We retrospectively studied 200 postoperated patients,from 1996 to 2000,who all had the preoperated MRI exams.All cases had histopathological evalutions.Both clinical presentation(age,sex,symtom,sign)and MRI findings(size,form,signal intensity,extension)were investigated.Results There was a great span of age with sixty percent owing for 35～55 years old.The male and female proportion was 1∶2 in PRL hormone-secreting pituitary adenoma.12 cases with luleinizing hormone-secreting and 2 cases with thyrotropin hormone-secreting pituitary adenoma were just male.In other types the incidences were similar in man and woman.Clinical symptom and sign present overlapping.The volume of tumor was from 0.5 cm?0.8 cm?1 cm to 6 cm?7 cm?8 cm with most multihormone adenoma's maxium dimension out of 5 cm.The signal intensity was not characteristic pre-and post-enhancement.No calcification could be seen.In term of extension,most was to suprasellar,then infrasellar,unilateral or bilateral sinus cavernous,last was to lower part of the third ventricle.Conclusion Different hormonesecreting pituitary adenomas have some speciality in clinical and MRI presentation.Although an accurate diagnosis can not be made according to it,more information have been obtained.

Objective To investigate the effect of nerve growth factor (NGF) and ciliary neurotrofic factor (CNTF) on the regeneration and functional recovery of sciatic nerves following sciatic nerve injuries (SNI). Methods The left sciatic nerves for 6 mm in 120 Wistar ratswere removed and divided randomly into 4 groups. Target muscular injection of NGF at concentration of 100 ng/kg and CNTF at 100 ng/kg was given to Group NGF+CNTF; CNTF at 100 ng/kg to Group CNTF; NGF at 100 ng/kg to Group NGF and equal quantity of normal saline to Group NS. Sciatic nerve functional index (SFI) measurement, morphometric analysis, S-100? and NF200 immunohistochemistry were performed for all the animals after operation. Results SFI, diameter and number of myelinated axons, and axonal quantity of S-100? and NF200 were significantly higher in Group NGF+CNTF than that in other groups. Conclusions Combined application of NGF and CNTF can enhance regeneration and functional recovery of sciatic nerves posterior to SNI.