Objective To understand the status quo of related knowledge,attitude and practice of religious belief among undergraduate nursing students and provide reference for education on religious belief.Methods A self-filled questionnaire on religious belief was used randomly among undergraduate nursing students in 4 medical universities in Wuhan.Results Most nursing students were inferior to the cognitive degree of related knowledge on belief religious,which was greatly related to the grade,nationality,parents’ religious belief and family residence factors.The notion of nursing students towards religious belief policy was nebulous.Most students could cognize the relationship between religion and science correctly.The university had minor effect on students’ religion view.Students contacted religious belief with various channels and the role of the network could not be underestimated.Undergraduate nursing students understood and pardoned their religious belief and other students’.Their religious belief was not the mainstream of current belief.The Buddhist belief of religion was in the first place.The majority of students went to religious activities not for the belief.Conclusions We should face the reality,examine rationally at this society and strengthen the social,school,family and individual guidance on religion together with related education coustruction.

The article describes the current situation of training mode of community nurses in community health service institutions in Hubei province from several aspects,including training object,training target,training base,teaching staff,training organization and government,training method and time,training curriculum,training effect evaluation and certification.The article suggests a multitude of factors influencing the training quality,such as formal training,dominant job training,and monotonous community training content and less training time.Overall,the government should be done something to increase government support and funding and accelerate the community nursing services and community nursing education.It is also essential to establish community nursing personnel training mechanism,improve and perfect the community health personnel training system,increase the construction of community nursing practice base and facilities and strengthen clinical practice skills.

To address the role of Pr1 gene in the process of Hirsutella sinensis infecting Hepialus gonggaensis, differential expression of subtilisin-like protease gene was detected. In the present study, Pr1 gene analogues from H. sinensis were obtained by PCR strategy using specific primers designed from conserved regions of Pr1 gene reported in the GenBank. Then we detected the changes in the expression of Pr1 gene before and after infecting H. gonggaensis using real-time quantitative PCR. We obtained the partial sequence of Pr1 gene with the length of 535 bp (GenBank accession: KC009680). Real-time PCR results showed that the expression level of Pr1 gene was significantly different among 8 samples (P < 0.01). Pr1 gene showed the obvious higher expression level (2-3 folds) after infecting the H. gonggaensis, suggesting that the Pr1 gene may play an important role in the process of H. sinensis infecting H. gonggaensis. The present study paves a way for further identification on infectivity assessment of H. sinensis.
Amino Acid Sequence ; Animals ; Hypocreales ; genetics ; metabolism ; pathogenicity ; Larva ; Lepidoptera ; microbiology ; Real-Time Polymerase Chain Reaction ; Subtilisin ; genetics ; metabolism

【Objective】 To investigate the management of adverse reactions to blood donation(ARBD) in blood services, so as to promote the surveillance of ARBD and improve the quality of blood donation service in Chongqing. 【Methods】 A questionnaire, involving the staff and facilities in blood donation sites as well as the prevention and treatment, the record and report, the following up and data related to ARBD was developed by Chongqing Society of Blood Transfusion in February 2019, and was issued to 18 blood services(1 blood center and its sub-center, 6 central blood stations and 11 hospital blood banks) in the Chongqing via email. The questionnaire was filled in and submitted before March 31 by management personnel participating in the investigation, and the data was collected, collated, revised and analyzed by Excel 2011. 【Results】 A total 19 questionnaires were collected, with the valid rate at 100%(19/19). 78.95%(15/19) of the blood services met the requirements of medical personnel allocation(>6 medical staff) when the number of daily blood collection was more than 60, and 100%(19/19)met the requirements of medical personnel allocation(2 to 6 medical staff) when the number of daily blood collection was less than 60. 89.47%(17/19) of the blood services were equipped with epinephrine hydrochloride, and 84.21%(16/19) with dexamethasone(an anti-allergic drug). There were significant differences in the allocation of other types of drugs. 100.00%(19/19) of the blood services formulated prevention and treatment measures concerning ARBD. In 2019, the incidence of ARBD in Chongqing was reported to be 0.54%(1 958 / 359 871), with the highestas [1.35%(223/16 543)] in subcenters and the lowest [0.32%(179/56 299)] in central blood centers (P<0.05). There was statistical significances in the incidences of ARBD reported by different blood stations(P<0.05). 【Conclusion】 The monitoring and management of ARBD among blood services in Chongqing should be further standardized in terms of staffing allocation, emergency drugs allocation and reporting, so as to gradually realize regional homogenization and ensure blood safety.

The theories of Shushu in the literature of Qin and Han periods are reorganized and expositions of foreigner scholars about ancient way of thought are consulted, and compared with the recordation about the ebb-flow of ying-qi and the length of meridians in ancient medical books to probe into the relationship between Shushu and these recordation. It is found that the ebb-flow of ying-qi and the length of meridians are completely conform with the principle of Shushu, i. e. the ebb-flow degrees of ying-qi and the length of meridians all are deduced from Shushu for building the close inseparable response relation between nature and humans. Because Shushu can not be verified, modern scientific methods are not suitable to the study on the ebb-flow of ying-qi.
Humans ; Mathematics ; Medicine, Chinese Traditional ; Meridians ; Qi ; Yin-Yang

To probe into the relation between spirit, pivot and the way for holding pivot. Find out the discussion about spirit, pivot and holding pivot in acupuncture from ancient books, including literature, history and philosophy, and analyze, check and compare with the theory of controlling spirit in acupuncture, and find out the internal relations. Controlling spirit is applied to hold pivot, the way for holding pivot includes finding fine evidence of diseases, holding chance for needling, seizing opportunity, using circumstances and considering the coming-and-going of evil, constitution of patients and reinforcing-reducing methods of acupuncture. The way of holding pivot generalizes the main thought of acupuncture treatment.
Acupuncture Therapy ; Humans ; Medicine, Chinese Traditional ; Spirituality

Ancient literatures on traditional Chinese medicine were retrieved, and theory of Biao (Branch) and Ben (Root) of meridians was studied from the aspect of acupuncture based on tracing of Weiqi (defensive qi). It was found that Biao and Ben were related with the gathering and dispersing of Weiqi (defensive qi) in 12 regular meridians. Therefore, observation of Weiqi (defensive qi) and tracing its running course is required before the application of acupuncture. Disease may cause migration of Weiqi (defensive qi) between Biao and Ben of meridians. Thus, points selection from Biao of meridians, Ben of meridians or both Biao and Ben can be adopted. Primary disease and secondary disease are held as the primary consideration for point selection from Biao or Ben. The idea can also be applied in treatment of complicated syndromes to clarify and simplify the situation. And during the treating process, physicians should proceed from Biao to Ben so as to conduct the anti-pathogenic qi to play its role in disease cure and health maintenance.
Acupuncture ; history ; Acupuncture Therapy ; history ; China ; History, Ancient ; Humans ; Medicine in Literature ; Meridians

OBJECTIVETo investigate the dynamic changes of surfactant protein-D (SP-D) and Clara cell protein (CC16) expressions in lung tissue and bronchoalveolar lavage fluid (BALF) of silica-treated rats.

METHODS80 rats were randomly divided into the control group and the silica group. The silicotic animal model was established by direct tracheal instillation of silica suspension into rat lungs surgically. On 7, 14, 21, 28 and 60 d after establishment of the animal model, eight rats in each group were sacrificed and lung tissue and BALF were collected. Lung tissue chip microarray was made in different time points after the silica was injected. Expressions of SP-D and CC16 on tissue microarray were detected with immunohistochemistry and quantified by Image-Pro Plus Version 4.5 for Windows(TM); The SP-D and CC16 levels of BALF were detected with western blot and quantified by Quantity One Version 4.6.2.

RESULTSSP-D expressed very little in alveolar type II and Clara cell intracytoplasmic of control group while its expression significantly increased after 7 d in silica group (P < 0.01) and it reached the peak on the 14 d, after this SP-D expression decreased gradually. CC16 was expressed strongly in intracytoplasmic and it expressed little in nucleus of Clara cell by bronchioles of control while it significantly decreased after 7 d in silica group (P < 0.01), and CC16 expression decreased gradually with the exposed silica time, which was correlated negatively among them (r(s) = -0.967, P < 0.01). On 7 d and 28 d, the SP-D levels of BALF in silica group were significantly higher than control (P < 0.01). Furthermore the SP-D levels of BALF on 28 d was significantly elevated than that on 7 d in silica group (P < 0.01). On 7 d and 28 d, the CC16 levels of BALF in silica group were significantly lower than control (P < 0.01). Moreover, CC16 levels of BALF on 28 d was significantly decreased than that on 7 d in silica group (P < 0.01).

CONCLUSIONThe dynamic changes of SP-D and CC16 protein expressed in lung tissue and bronchoalveolar lavage fluid could be induced by silica exposure and are related with the silica exposure time.

Animals ; Bronchoalveolar Lavage Fluid ; chemistry ; Disease Models, Animal ; Lung ; metabolism ; Male ; Pulmonary Surfactant-Associated Protein D ; metabolism ; Rats ; Rats, Wistar ; Silicosis ; metabolism ; Uteroglobin ; metabolism

OBJECTIVETo probe into the reflection of "artery" phenomenon in human channels.

METHODSFind out the discussion about "artery" from ancient medical books and compare with Chinese ancient philosophy thoughts.

RESULTSIn TCM, "artery" has been used as reference in differentiating channels. The circulation system of channels is possibly established on the expectation of "to and fro circulation" in the Chinese philosophy, and the power of channels originates from the qi stored between the kidney, and the pricking blood therapy was used for treatment of many kinds of diseases in ancient times.

CONCLUSIONStudy on channels should pay attention to this link of "artery".

Arteries ; anatomy & histology ; physiology ; Humans ; Medicine, Chinese Traditional ; Meridians

UNLABELLEDFind out the discussion about sensation response and controlling spirit in acupuncture from ancient books,

METHODSFind out the discussion about sensation response and controlling spirit in acupuncture from ancient books, including literature, history and philosophy, and analyze, check and compare with the theory of controlling spirit in acupuncture to find out the internal relations.

RESULTSControlling spirit is based on the theory of sensation response, and it is a special training and a treatment course which forwardly controlled by acupuncturist.

CONCLUSIONThe therapy of controlling spirit in acupuncture incarnates that traditional Chinese medicine advocates the experience of life and pays attention to the amalgamation of lives.

Acupuncture Therapy ; Humans ; Medicine, Chinese Traditional ; Sensation ; Spirituality