OBJECTIVE:To investigate the improvement effects of Zhuanggushenjin capsules on osteoporosis induced by reti-noic acid in mice. METHODS:84 mice were randomly divided into normal control group,model control group,positive control group [Gushukang granules,5 g(crude drug)/kg] and Zhuanggushenjin capsules high-dose,medium-dose and low-dose groups [7.80,3.90,1.95 g(crude drug)/kg]. Except for normal control group,those groups were given retinoic acid(90 mg/kg)to induce osteoporosis model. 7 days after modeling,all drug groups were given relevant medicine intragastrically,normal control group and model control group given normal saline intragastrically ,once a day,for consecutive 14 days. ALP activity and the contents of cal-cium and phosphorus in serum and thigh-bone were determined. The body weight, the dry weight, length and diameter of thigh-bone were determined,and organ coefficients (spleen,testicle,ovarium and uterus) were calculated. RESULTS:Compared with normal control group,the activity of ALP in serum of mice increased in model control group,while the contents of calcium and phosphorus,body weight,length and diameter of thigh-bone decreased significantly;spleen coefficients increased,while testi-cle and uterus coefficients decreased(P<0.05 or P<0.01). Compared with model control group,the contents of calcium and phos-phorus increased in Zhuanggushenjin capsules groups,while spleen coefficient decreased;the activity of ALP in serum of mice de-creased in high-dose and medium-dose groups,while body weight,the length of thigh-bone,testicle and uterus coefficients in-creased;diameter of thigh-bone increased in high-dose group (P<0.05 or P<0.01). CONCLUSIONS:Zhuanggushenjin capsules can improve retinoic acid-induced osteoporosis in mice to certain extent.