Objective To analyze the complications of patients with gastric cancer after laparoscopic radical operation and its influencing factors.Methods The clinical data of 297 patients with gastric cancer undergoing laparoscopic radical gastrectomy was retrospective analysized,the occurrence of postoperative complications and influencing factors were analysized.Results In 297 patients,37 cases occurred postoperative complications,the postoperative complication rate was 12.46%.Single factor analysis,patients with onset age,BMI (body mass index (BMI),surgery preoperative comorbidity,operation time,tumor size,surgical experience and lymph node metastasis and the laparoscopic radical operation of gastric cancer patients with postoperative complications are closely related (t =8.238,9.728,9.453,8.042,6.355,11.597,6.455,all P <0.05).Multi factor logistic regression analysis,showed that BMI,preoperative complications,tumor size,surgical experience and lymph node metastasis were independent risk factors for postoperative complications (P <0.05 ).And the patients with complications after anal exhaust time of (3.91 ±1.63)was later than the patients without complications(t =7.863,P <0.05),the length of stay of (20.14 ± 6.28)d was longer than that of the patients without complications (t =9.293,P <0.05).Conclusion There are complications after laparoscopic radical gastrectomy for gastric cancer,the independent risk factors of complications are BMI,preoperative complications,tumor size,surgical experience and lymph node metastasis.

Multidrug regimens and corresponding drug interactions cause many adverse reactions and treatment failures. Drug efflux transporters: P-glycoprotein (P-gp), multidrug resistance associated protein (MRP) and breast cancer resistance protein (BCRP) in conjunction with metabolizing enzymes (cytochrome P450, CYP450) are major factors in such interaction. In recent years, a large number of studies have shown that P-gp plays a role in the oxidative metabolism of its substrates that are also substrates of CYP3A4. Combined actions of P-gp and CYP3A could account in some part for the low oral bioavailability determined for many of these dual substrates. P-gp along with efflux transporters (MRP and BCRP) having overlapping substrate specificity plays critical role in drug disposition. The relationship between MRP or BCRP and CYP3A is similar to that between P-gp and CYP3A. In this paper, we summarize the classification of efflux transporters, the main metabolizing enzymes CYP3A, clinical significance interactions mediated by efflux transporters and CYP450 enzymes and in vitro studies.

[Summary] With an increasing higher failure rate from 50%to 70%after simple sutured surgery for rotator cuff tears , scholars have put forward the patch augmented biomechanics for healing massive tears .Research hotspots lie in the material structural and physiological properties .There are two types of rotator cuff patches , which are synthetic materials ( non-degradable and degradable type ) and biological rotator cuff patch ( autologous or allogeneic tissue , xenogeneic material ) .The synthetic materials rotator cuff patch is able to provide powerful biomechanics but has severe immune rejection , and the biomaterials patch provides excellent histological reconstruction in rotator cuff and accurate regulation of biological activity of metabolic degradation but lower biomechanical maximum tensile strength .This article reviewed the current common problems and progress in the application of the rotator cuff patches .

Carrier State ; immunology ; prevention & control ; China ; epidemiology ; Hepatitis B ; epidemiology ; prevention & control ; Hepatitis B Vaccines ; immunology ; Humans ; Prevalence ; Vaccination

Objective: To study the effect of glutamate on the expression of vascular endothelial growth factor (VEGF) mRNA and protein in cultured rat astrocytes. Methods: Cultured rat astrocytes were randomly divided into 6 groups: control group (C), glutamate group (G), QA group (Q), DCG-IV group (D), L-AP4 group (L) and glutamate+MCPG group (G+M). Cells were cultured under nomoxic condition (95% air, 5% CO2). RT-PCR and ELISA methods were used to detect the expression of VEGF mRNA and protein in cultured astrocytes, respectively. G+M group was preincubated with 1mM MCPG for 30 min prior to the stimulation with glutamate. There were 7 time points at 0, 4, 8, 12, 16, 24 and 48 h in each group except G+M group. Results: The expression of VEGF mRNA and protein did not differ significantly among D group, L group and C group. Different from that in C group, the expression of VEGF mRNA and protein could be enhanced both in a dose-dependent and time-dependent manner in G group and Q group. Meanwhile, the enhanced expression of VEGF mRNA and protein in G group was completely suppressed by MCPG after 24 h. Conclusion: Glutamate can increase the expression of VEGF mRNA and protein in cultured astrocytes, which may be due to the activation of group I metabotropic glutamate receptors in astrocytes.

Objective: To study the effect of glutamate on the expression of vascular endothelial growth factor (VEGF) mRNA and protein in cultured rat astrocytes. Methods: Cultured rat astrocytes were randomly divided into 6 groups: control group (C), glutamate group (G), QA group (Q), DCG-IV group (D), L-AP4 group (L) and glutamate+MCPG group (G+M). Cells were cultured under nomoxic condition (95% air, 5% CO2). RT-PCR and ELISA methods were used to detect the expression of VEGF mRNA and protein in cultured astrocytes, respectively. G+M group was preincubated with 1mM MCPG for 30 min prior to the stimulation with glutamate. There were 7 time points at 0, 4, 8, 12, 16, 24 and 48 h in each group except G+M group. Results: The expression of VEGF mRNA and protein did not differ significantly among D group, L group and C group. Different from that in C group, the expression of VEGF mRNA and protein could be enhanced both in a dose-dependent and time-dependent manner in G group and Q group. Meanwhile, the enhanced expression of VEGF mRNA and protein in G group was completely suppressed by MCPG after 24 h. Conclusion: Glutamate can increase the expression of VEGF mRNA and protein in cultured astrocytes, which may be due to the activation of group I metabotropic glutamate receptors in astrocytes.

Multidrug regimens and corresponding drug interactions cause many adverse reactions and treatment failures. Drug efflux transporters: P-glycoprotein (P-gp), multidrug resistance associated protein (MRP) and breast cancer resistance protein (BCRP) in conjunction with metabolizing enzymes (cytochrome P450, CYP450) are major factors in such interaction. In recent years, a large number of studies have shown that P-gp plays a role in the oxidative metabolism of its substrates that are also substrates of CYP3A4. Combined actions of P-gp and CYP3A could account in some part for the low oral bioavailability determined for many of these dual substrates. P-gp along with efflux transporters (MRP and BCRP) having overlapping substrate specificity plays critical role in drug disposition. The relationship between MRP or BCRP and CYP3A is similar to that between P-gp and CYP3A. In this paper, we summarize the classification of efflux transporters, the main metabolizing enzymes CYP3A, clinical significance interactions mediated by efflux transporters and CYP450 enzymes and in vitro studies.
ATP Binding Cassette Transporter, Sub-Family G, Member 2 ; ATP-Binding Cassette Transporters ; metabolism ; ATP-Binding Cassette, Sub-Family B, Member 1 ; metabolism ; Biological Availability ; Cytochrome P-450 CYP3A ; metabolism ; Cytochrome P-450 Enzyme System ; metabolism ; Drug Interactions ; Humans ; Multidrug Resistance-Associated Proteins ; metabolism ; Neoplasm Proteins ; metabolism ; Substrate Specificity

Diagnostic Imaging ; Echocardiography ; Humans ; Magnetic Resonance Imaging ; Myocarditis ; diagnosis ; Radionuclide Imaging

[Abstract] Objective To investigate the effect of post-traumatic stress disorder posttraumatic stress disorder (PTSD) on blood pressure, heart rate, and heart rate variability. Methods Seventy-eight patients with admitted in the 986th Hospital of PLAAF (former the 323th Hospital of PLA) from May 2016 to January 2018 and our hospital from February 2018 to May 2018 were selected as the study group, and 72 patients with non stress disorder in the same period served as the control group A, and 74 healthy persons enrolled in the same period served as the control group B. The blood pressure, heart rate, and heart rate variability of the three groups were monitored and compared between the groups. Results The systolic and diastolic blood pressures of the patients in the study group were significantly higher than those in the control group A and control group B, and the control group A was significantly higher than the control group B (P<0.05). The 24h total heart rate, average heart rate, fastest heart rate, and slowest heart rate of the patients in the study group were significantly higher than those of the control group A and control group B (P<0.05), but there was no significant difference between the control group A and control group B (P<0.05). The standard deviation of normal to normal intervals (SDNN), SDANN, and SDNN index of the patients in the study group were significantly lower than those of the control group A and control B, and the control group A was significantly lower than the control group B (P<0.05), however, there was no significant difference between the two groups in rMSSD and PNN50 (P>0.05). Conclusion PTSD can raise blood pressure, increase heart rate, and lead to abnormal autonomic nervous tone and imbalance of regulatory function.

0.05);but the levels of OPN in stageⅢ(84.0% and 88.0%)were significantly higher than those of stageⅡ(P