No abstract available.
Esophageal Motility Disorders* ; Gastroesophageal Reflux*

Objective To investigate the association between M235T polymorphism of angiotensinogen (AGT) gene and cerebral infarction(CI) in Chinese.Methods AGT gene M235T polymorphism was examined in 75 cases with CI and 48 healthy controls by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP).Results The frequencies of AGT gene T235 allele and 235TT genetype were 0.78 and 0.64 respectively,compared with those of the control group (0.604 and 0.375 respectively ),the difference was significant(? 2=8.82, P

No abstract available.
Constipation* ; Diagnosis*

Thirty strains of seven Candida species from CICC(China Center of Industrial Culture Collection)were studied. The strains were differentiated by ITS1 region PCR-SSCP fingerprinting analysis and ITS2 region PCR-SSCP fingerprinting analysis. Results showed that both ITS1 region and ITS2 region were able to differentiate the seven species of Candida clearly. Contrasting the maps and effects on the identification of Candida species of ITS1 region with that of ITS2 region, result indicated that on the identification of Candida species the application of ITS2 region was better than ITS1 region.

No abstract available.
Gastrectomy* ; Humans ; Retrospective Studies*

Objective To construct the adenoviral vector carrying Mus-?NGF gene and to study the expression of the gene in the marrow mesenchymal stem-cells(MSCs).Methods Mus-?NGF cDNA was cloned from mice glandulae maxillaries whole mRNA by reverse-transcription polymerase-chain-reactions.The Mus-?NGF cDNA was positively cloned into the adenoviral shuttle vector pAdtrack carrying cytomegalovirus promoter(CMV)and tag-protein(GFP),and then homologous recombined into competent BJ5 183 cells with the adenoviral backbone plasmid pAdeasy-1.The recombinant adenoviral plasmid was identified by restriction enzymes and transfected into HEK293 cells to package and amplify recombinant adenoviral particles which was capable of infecting and would express ?NGF and GFP proteins.After the adenovirus infected the MSCs,the expression was observed via fluorescent microscrope and detected using immunocytochemical method.The secretion of NGF was detected by WB.Results The recombinant adenoviral vector carrying Mus-?NGF gene was constructed and confirmed by restriction endonuclease enzyme analysis.The relucent green fluor-light would be observed in the MSCs.IHC showed that Mus-?NGF gene was exactly transcripted and expressed in the MSCs,and the secretion of NGF was confirmed.Conclusion The exogenous gene,such as ?NGF,an be imported into the MSCs efficiently by recombinant adenoviral vector,and can be expressed and secreted successfully in the cells.It improves foundation for the celluar transplant and gene intervention of MSCs expressing NGF in inner ear.

Objective To develop an analyzing technique of single nucleotide polymorphism(SNP) based on non-probe real-time quantitative PCR, and to explore the relationship between SNP in human adiponectin (APM1) gene and type 2 diabetes (T2DM).Methods Design primers with specific 3′-terminal nucleotides and perform Real-time PCR using SYBR Green dye. Genotypes were determined by comparing the amplification efficiency of each pair of primer and verified by sequencing. SNPs 45 and 276 in APM1 of 20 T2DM, 24 obesity and 28 healthy persons were analyzed. Results This technique could discriminate genotype as efficient as sequencing. The genotype of APM1 at +45 site was significantly correlated with T2DM (P

Objective To determine the effect of resurfacing completely degloved thumbs by free perforator flaps in a spiral fashion.Methods A retrospective case series study was made on 22 patients with completely degloving injury of the thumb treated by different free perforator flaps in a spiral fashion from June 2009 to December 2014,including 19 male and 3 female patients at the age of 18-46 years (mean,27.8 years).A total of 13 patients were reconstructed using the medial arm perforator flaps ranging in size from 8.0 cm × 3.0 cm-14.5 cm × 3.5 cm,and 9 patients were reconstructed using the peroneal artery perforator flaps ranging in size from 6.0 cm × 3.5 cm-21.0 cm × 10.0 cm.Follow-up was made to evaluate the sensation,appearance and function of the thumbs and the recovery of flap donor sites.Results All flaps survived completely,with no occurrence of postoperative vascular crisis and infection.Only one medial arm flap and one soleus perforator flap distal flap showed blood flow disorder in the distal sections and were healed uneventfully after conservative therapy.Function and cosmetic recovery of the reconstructed thumbs were good.Follow-up ranged from 9 to 22 months (mean,14.5 months).Twopoint discrimination test of the flaps was recovered to 6-13 mm.No linear scar contracture was left.Flap donor site wound healed primarily.All patients were satisfied with the overall results.Conclusion The free perforator flap in a spiral fashion can avoid linear scar contracture,maximally decrease donor site morbidity and achieve satisfactory functional recovery and good aesthetic restoration,indicating a good candidate for reconstruction of thumb degloving injury.

CT may be valuably used indetermination of proptosis. Normal value of eyeball protrusion was measured innormal Koreans using CT. Among 86 Koreans, 26 have normal both eyes, but 60 have one normal eye with one abnormaleye (chiefly, proptosis and and eyeball tumor) The results of 112 eyes are as follows; Absolute measurement of“a”, that is, the distance between cornea & IZL (interzygomatic line) is 16.0±3.4mm in male (for 63 eye),15.7±4.0 mm in female for 49 eyes. Ratio of “a” over “A” (A-P diameter of eyeball) is 57±12% is male (for 63eye), 57±14% in female (for 49 eye) Discrepancy of eyeball protrusion between both eyes is 0.67±1.5mm (for 26adults) Practically, normal range of eyeball prototrusion(“a”) is 12.6mm< male< 19.4mm, 11.7mm
Adult ; Cornea ; Exophthalmos ; Female ; Humans ; Male ; Reference Values

Objective To compare the influence of the dynamic device(DD) of pedicle screw fixation and its equivalent rigid device (RD) on bone mineral density and bone structure of the vertebrae. Methods 24 goats were randomly divided into 4 groups: control group, single posterolateral fusion group, dynamic device with posterolateral fusion group and rigid device with posterolateral fusion group. After 24 weeks postoperative observation, the animals were sacrificed and the fixed lumbar spines were harvested. The bone mineral density (BMD) of these harvested specimens was measured by quantitative computed tomography (QCT). Then the L3 segment was embedded in methyl methacrylate and sectioned after alcoholic dehydration. Undecalcified slides (50 ?m in thickness) were stained with toluidine blue. Quantitative histologic analysis was performed in a semiautomatic way. The static parameters consisted of trabecular bone volume (TBV), the mean trabecular plate thickness (MTPT) and mean trabecular spacing (Tb.Sp). The main dynamic histomorphometric parameter, bone mineralization rate (MAR), was calculated by measuring the mean distance between two flurochrome labelings. Results The BMD in the rigid device group decreased significantly compared with control group (P