1. The internal iliac artery was investigated in 100 cases. In 23 cases this arterywas divided into anterior and posterior trunks, while in the remaining 77 cases suchtrunks were absent. 2. The mode of origin of the superior, inferior gluteal arteries and the internalpudendal artery from the internal iliac artery, may be classified into five principal typesand four subtypes. Among them the second type is most frequent (54 cases), the firstand the third type are next in order, their occurrence are 24 cases and 17 cases respec-tively. The fifth type possesses 2 common trunks and occurs in 4 cases. 3. The obturator artery, according to its origin, is divided into three types. Thefirst type, arises from the internal iliac artery, comprises the largest number (81 cases).The second type, arises from the external iliac artery, is next in number (15 cases). Thethird type has two obturator arteries arising from the internal and external arteries (4cases) respectively. 4. The iliolumbar artery arises from the internal iliac artery (54 cases), some fromthe superior gluteal artery (12 cases). 5. The lateral sacral artery, mostly arises from the superior gluteal artery, occursfrom 1--3 branches. There are 2 branches in 43 cases, one branch in 32 and threebranches in 25 cases.

Child ; Child, Preschool ; Drug Therapy ; Humans ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; drug therapy ; Remission Induction ; methods ; Survival Analysis ; Treatment Outcome

Objective:To observe the effects of double-stranded small interfering RNA (siRNA) of the silent mating-type infor-mation regulation 2 homolog 1 (SIRT1) on the cell proliferation, cell cycle progression, and expression levels of the cell cycle negative regulators. These regulators include P21, P27, and phosphorylated retinoblastoma (PRb) proteins present in prostate cancer PC3 cells. This work further aims to explore the possible underlying mechanism for such effects. Methods:PC3 cells were cultured in vitro and then randomly divided into the mock group, scramble siRNA transfected group, and SIRT1 siRNA-transfected group. SIRT1 siRNA ef-ficiency was examined through reverse transcription polymerase chain reaction and Western blot analysis. The inhibitory rate of PC3 cell growth was determined through a methyl thiazolyl tetrazolium assay, and the cell cycle was investigated with the use of flow cytom-etry. The P21 and P27 protein expression levels and PRb status were determined by Western blot assay. Results:Compared with those of the mock and scramble siRNA groups, the expression levels of SIRT1 mRNA and protein significantly decreased in SIRT1 siR-NA-transfected cells. In addition, the inhibitory rate of PC3 cell growth was markedly increased, and the cell cycle of the PC3 cells was arrested at the G1 stage. The expression levels of negative cell cycle regulators, including P21 and P27 protein levels increased, whereas Rb protein phosphorylation was inhibited in SIRT1 siRNA-transfected PC3 cells. Conclusion: SIRT1 RNA interference inhibits PC3 cell growth and arrests cell cycle progression through the upregulation of the P21 and P27 proteins and the inhibition of Rb protein phosphorylation.

ObjectiveTo seek an effective protocol fortreating spinal cord injury-induced limb dysfunction by comparing the therapeutic effects of electroacupuncture at Huatuo jiaji points plus Du meridian points versus conventional electroacupuncture. MethodForty patients with spinal cord injury-induced limb dysfunction were allocated, using a random number table, to treatment and control groups, 20 cases each. Both groups were treated with ganglioside and routine rehabilitation therapy. In addition,the treatment group received electroacupuncture at Huatuo jiaji points and Du meridian points and the control group, conventional acupuncture. Lower limb function was assessed using ASIA Motor Score, Sensory Score and the MBI in the two groups of patients before treatment and after one and two courses. The clinical therapeutic effects were evaluated.ResultThere was a statistically significant pre-/post-treatment difference in lower limb dysfunction in both treatment and control groups (P<0.05). The improving effect was better in the treatment group than in the control group (P<0.05,P<0.01).ConclusionElectroacupuncture at Huatuo jiaji points and Du meridian points has a definite therapeutic effect on spinal cord injury-induced limb dysfunction. It is superior to conventional electroacupuncture.

Objective To study CT findings of sacral tuberculosis.Methods CT findings of sacral tuberculosis confirmed pathologically in 13 cases were analysed combined with literature review.Results The S 1～2 tuberculosis was most common.The types of sacrum destruction were osteolytic and bone destruction along the sacral foramen.The cold abscess were distributed at the presacral,unilateral iliac fossa and/or the gluteal region.Three patients(23.1%) were associated with sacroiliac joint tuberculosis(right 2,left 1).Conclusion In the patients with sacral tuberculosis the patterns of sacrum destruction,region of cold abscess and its relationship with the neighboring structures can be well showed by CT,and it can help surgeons to choose the appropriate surgical approach.

Objective To develop models of epileptic discharge by activating group Ⅰ metabotropic glutamate receptors (mGluR) or by blocking gamma-aminobutyric acid (GABA) receptors on rat hippocampal slices. Methods Rat hippocampal slices were exposed to mGluR group Ⅰ specific agonist dihydroxyphenylglycine (DHPG) or to GABAA receptor antagonists bicuculline methiodide(BMI), and single pyramidal cell in the CA3 region of the slice was recorded by whole cell patch clamp technique. Results Exposure to DHPG or BMI resulted in the induction of spontaneously occurring epileptic discharge in the CA3 region of rat hippocampal slice, and there was no significant difference in the frequency of discharge between them(P>0.05). Conclusion Epileptic discharge can be generated in vitro in response to a loss of balance between excitatory and inhibitory influences.

Central Nervous System ; pathology ; Central Nervous System Neoplasms ; drug therapy ; Child ; Child, Preschool ; Humans ; Methotrexate ; therapeutic use ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; drug therapy

ObjectiveTo solve the problem about the shortage of the human specimen,we aimed to reform the anthropotomy experiment teaching mode.MethodsThe nursing specialty students in Grade 2010 were randomly selected with 70 students traditional groups and 71 students teaching reform groups.The students of traditional groups were taught the key contents mainly in class time and were taught on the normally-exposed bottle specimen,while the students of teaching reform groups spend the primary class time on self-study.First,the students of teaching reform groups were informed of key contents and sealed bottle specimen. Second, the students of teaching reform groups observed only sealed bottle specimen in accordance with the main points of teaching materials.ResultsTeaching reform groups' average score of specimen examination is ( 81.30 ± 13.71 ),and theory examination is ( 70.83 ± 11.49 ),while the tradition groups' average score of specimen examination is ( 71.47 ± 16.00 ),and theory examination.is ( 66.29 ± 12.15 ).Results of teaching reform groups are much better than results of tradition groups in specimen test and theory test.Learning interest of teaching reform groups are elevated remarkably.They have improved much in their learning ability,cooperation ability,sense of group honor and the ability of originative thinking.ConclusionTeaching reform students' test result and comprehensive efficiency are promoted.The discussion-based experiment teaching mode is worth spreading.

BACKGROUND: Plenty of materials have been used as stent in total auricular reconstruction with varying advantages and disadvantages. The in vitro prepared tissue engineered complex cartilage stent has been not applied in clinic.OBJECTIVE: To observe the ultrastructure changes of surrounding tissues following total auricular reconstruction with nickel-titanium (NiTi) shape memory alloy stent in vivo.DESIGN, TIME AND SETTING: The randomized, controlled, animal experiment was performed in the Central Laboratory of EENT Hospital of Fudan University, Anhui Provincial Hospital and Department of Nuclear Medicine, Huashan Hospital of Fudan University from April 2004 to October 2005.MATERIALS: A total of 12 New Zealand rabbits were randomized divided into the skin-expanded and unexpanded groups, with 6 animals in each group.METHODS: Each animal underwent subcutaneous implantation with a NiTi alloy stent like human auricle. In the skin-expanded group, the stent was implanted after skin expansion, while no treatment in the unexpanded group.MAIN OUTCOME MEASURES: The ultrastructure changes of surrounding tissues were detected by scanning electron microscope at months 6 and 12 after implantation.RESULTS: Structure of nickel-titanium alloy implant stent make fibrovascular ingrowth more easily. The surface of nickel-titanium alloy stent was smooth without corrosion. There was no evidence of infection in the host tissue. Microstructure of Ni-Ti stent wires showed no morphologic and functional changes after subcutaneously implanted in vivo, and the difference between 2 groups had no significance.CONCLUSION: NiTi shape memory alloy stent can smoothly pass the process of fibrovascularization with less complication.According to the examination by SEM, the nickel-titanium stent showed good biocompatibility.

Long-term prognosis of stroke rehabilitation is associated with a number of factors, such as age, sex, race, lesion sites and sides, lesion nature and various complications. Under-standing these related factors not only contribute to clinical judgement of prognosis, but also provide the basis for the targeted rehabilitation therapy.