1.Effects of bilateral superficial cervical plexus block on sevoflurane consumption during thyroid surgery under entropy-guided general anesthesia: a prospective randomized study.
Sudheesh KANNAN ; Nethra S SURHONNE ; Chethan Kumar R ; Kavitha B ; Devika Rani D ; Raghavendra Rao R S
Korean Journal of Anesthesiology 2018;71(2):141-148
BACKGROUND: Bilateral superficial cervical plexus block (BSCPB) provides good postoperative analgesia, but its effect on anesthetic consumption is unknown. This study evaluated the effects of BSCPB on sevoflurane consumption during thyroid surgery. METHODS: Fifty patients were randomly allocated into groups A and B of 25 each in this prospective double-blind study. Group A received BSCPB with 20 ml 0.25% bupivacaine, whereas group B received 20 ml saline immediately before entropy-guided general anesthesia. Intraoperative hemodynamic parameters, end-tidal sevoflurane concentration, minimum alveolar concentration, and sevoflurane consumption were recorded. Postoperative pain was assessed using a visual analog scale, and the time of the first request for analgesia was noted. All side effects were recorded. RESULTS: Demographics were comparable. Mean sevoflurane consumption [for 30 min: group A = 7.2 (1.1) ml, group B = 8.8 (2.0) ml, P = 0.001; for 60 min: group A = 13.5 (1.7) ml, group B = 16.5 (3.9) ml, P = 0.002] and mean end-tidal sevoflurane concentration [for 30 min: group A = 1.2% (0.2%), group B = 1.4% (0.2%), P = 0.008; for 60 min: group A = 1.2% (0.1%), group B = 1.4% (0.2%), P = 0.010] were significantly lower in group A. Patients in group A had a longer duration of analgesia [361.6 (79.5) min vs. 151.0 (60.2) min, P < 0.001] compared to those in group B. CONCLUSIONS: Preinduction BSCPB during thyroid surgery significantly reduced sevoflurane consumption and increased the duration of postoperative analgesia.
Analgesia
;
Anesthesia, General*
;
Bupivacaine
;
Cervical Plexus Block*
;
Cervical Plexus*
;
Demography
;
Double-Blind Method
;
Entropy
;
Hemodynamics
;
Humans
;
Nerve Block
;
Pain, Postoperative
;
Prospective Studies*
;
Thyroid Gland*
;
Thyroidectomy
;
Visual Analog Scale
2.An in vivo electromyographic evaluation of pain relief using different therapies in masticatory myalgia patients
Parvathi K. BALAKRISHNAN ; Sowmya M. KUMAR ; Purushotham CHIPPALA ; Chethan HEGDE
Journal of the Korean Association of Oral and Maxillofacial Surgeons 2020;46(5):321-327
Objectives:
This study is aimed to evaluate and compare the effect of moist heat fomentation therapy with ultrasound therapy in patients with the masticatory myalgia.
Materials and Methods:
The study was conducted on 42 patients with masticatory myalgia, dividing them into two groups; Group A (21 patients), received moist heat therapy and Group B (21 patients), received ultrasound therapy for seven effective days. Prior and after the treatment the numeric rating scale (NRS) and the electromyography (EMG) scores were recorded and compared. The observations were analyzed clinically and statistical support was taken to assess the NRS and EMG data.
Results:
Irrespective of the groups, patients testified a significant reduction in pain after the treatment. From the EMG readings; even though the standard deviation for each group was varied considerably, EMG recorded an improved muscle activity. Statistical analysis was used to assess and identify the best treatment methodology between the two modalities.
Conclusion
From the statistical analysis, it is concluded that, though both the therapies had significantly reduced the symptomatic response, it is moist heat fomentation that improved muscle activity both statistically and clinically in comparison to ultrasound.
3.Inhibition of protein kinases by anticancer DNA intercalator, 4-butylaminopyrimido4',5':4,5thieno(2,3-)quinoline.
HeggoduG Rohit KUMAR ; Chethan S KUMAR ; Hulihalli N Kiran KUMAR ; Gopal M Advi RAO
Acta Pharmaceutica Sinica B 2017;7(3):303-310
Targeting protein kinases (PKs) has been a promising strategy in treating cancer, as PKs are key regulators of cell survival and proliferation. Here in this study, we studied the ability of pyrimido[4',5':4,5]thieno(2,3-)quinolines (PTQ) to inhibit different PKs by performing computational docking andscreening. Docking studies revealed that 4-butylaminopyrimido[4',5':4,5]thieno(2,3-)quinoline (BPTQ) has a higher order of interaction with the kinase receptors than other PTQ derivatives.screening confirms that BPTQ inhibits VEGFR1 and CHK2, with the ICvalues of 0.54 and 1.70 µmol/L, respectively. Further, cytotoxicity of BPTQ was measured by trypan blue assay. Treatment with BPTQ decreased the proliferation of HL-60 cells with an ICvalue of 12 µmol/L and induces apoptosis, as explicated by the fall in the mitochondrial membrane potential, annexin V labeling and increased expression of caspase-3. Taken together, these data suggest that BPTQ possess ability to inhibit PKs and to induce cell death in human promyelocytic leukemia cells.
4.In Vitro Evaluation of Human Demineralised Teeth Matrix on Osteogenic Differentiation of Gingival Mesenchymal Stem Cells
Dhanashree Deshpande ; Arvind Karikal ; Chethan Kumar ; Basavarajappa Mohana Kumar ; Veena Shetty
Archives of Orofacial Sciences 2022;17(2):247-258
ABSTRACT
The use of tooth-derived material as a scaffold has gained attention recently due to its ease of availability
and bioactive properties. Hence, the objective of this study was to determine in vitro interaction of human
gingival mesenchymal stem cells (hGMSCs) with human demineralised teeth matrix (hDTM) on osteogenic
potential with or without osteogenic inducers. The hGMSCs were established and characterised on their
morphology, proliferation, population doubling time (PDT), viability, colony-forming ability, expression of
cell surface markers and adipogenic differentiation. Further, the effect of hDTM on the biocompatibility
and osteogenic differentiation ability of hGMSCs was evaluated. The hGMSCs displayed a fibroblast-like
appearance and exhibited a greater proliferative activity. The cells showed > 91% viability, and PDT varied
between 39.34 hours and 62.59 hours. Further, hGMSCs indicated their propensity to form clusters/
colonies, and expressed the markers, such as CD29, CD44, CD73 and CD90, but were negative for CD34
and CD45. When treated with adipogenic induction medium, hGMSCs were able to exhibit the formation
of neutral lipid vacuoles. The hGMSCs cultured with hDTM did not show any cytotoxic changes including
morphology and viability. Mineralisation of calcium nodules was observed in hGMSCs when cultured in
osteogenic induction (OI) medium as an indication of osteogenesis. hGMSCs when cultured with hDTM
confirmed the presence of a mineralised matrix. Further, when the cells were cultured with hDTM along
with OI, they showed slightly enhanced differentiation into osteocytes. In conclusion, hGMSCs were shown
to be biocompatible with hDTM, and demonstrated their enhanced osteogenic potential in the presence of
hDTM and osteogenic supplements.
Mesenchymal Stem Cells
;
Dental Pulp--cytology
;
Dentin
5.In Vitro Evaluation of Human Demineralised Teeth Matrix on Osteogenic Differentiation of Gingival Mesenchymal Stem Cells
Dhanashree Deshpande ; Arvind Karikal ; Chethan Kumar ; Basavarajappa Mohana Kumar ; Veena Shetty
Archives of Orofacial Sciences 2022;17(2):247-258
ABSTRACT
The use of tooth-derived material as a scaffold has gained attention recently due to its ease of availability
and bioactive properties. Hence, the objective of this study was to determine in vitro interaction of human
gingival mesenchymal stem cells (hGMSCs) with human demineralised teeth matrix (hDTM) on osteogenic
potential with or without osteogenic inducers. The hGMSCs were established and characterised on their
morphology, proliferation, population doubling time (PDT), viability, colony-forming ability, expression of
cell surface markers and adipogenic differentiation. Further, the effect of hDTM on the biocompatibility
and osteogenic differentiation ability of hGMSCs was evaluated. The hGMSCs displayed a fibroblast-like
appearance and exhibited a greater proliferative activity. The cells showed > 91% viability, and PDT varied
between 39.34 hours and 62.59 hours. Further, hGMSCs indicated their propensity to form clusters/
colonies, and expressed the markers, such as CD29, CD44, CD73 and CD90, but were negative for CD34
and CD45. When treated with adipogenic induction medium, hGMSCs were able to exhibit the formation
of neutral lipid vacuoles. The hGMSCs cultured with hDTM did not show any cytotoxic changes including
morphology and viability. Mineralisation of calcium nodules was observed in hGMSCs when cultured in
osteogenic induction (OI) medium as an indication of osteogenesis. hGMSCs when cultured with hDTM
confirmed the presence of a mineralised matrix. Further, when the cells were cultured with hDTM along
with OI, they showed slightly enhanced differentiation into osteocytes. In conclusion, hGMSCs were shown
to be biocompatible with hDTM, and demonstrated their enhanced osteogenic potential in the presence of
hDTM and osteogenic supplements.
Mesenchymal Stem Cells
;
Dental Pulp--cytology
;
Dentin