1.Extract of buckwheat sprouts scavenges oxidation and inhibits pro-inflammatory mediators in lipopolysaccharide-stimulated macrophages (RAW264.7).
Rajendra KARKI ; Cheolho PARK ; Dongwook KIM
Journal of Integrative Medicine 2013;11(4):246-52
Buckwheat has been considered as a potential source of nutraceutical components on the world market of probiotic foodstuffs. The purpose of this study was to evaluate the effects of tartary buckwheat (Fagopyrum tataricum) sprouts on oxidation and pro-inflammatory mediators.
2.Natural infection of Crenosoma vulpis (Nematoda: Crenosomatidae) in an urban Korean dog.
Sungjin CHOI ; Cheolho SIM ; Hyeon Cheol KIM ; Ho Jung CHOI ; Bae Keun PARK
Korean Journal of Veterinary Research 2014;54(2):127-129
A male, 3.5 month old Pomeranian dog was diagnosed with a natural infection of Crenosoma (C.) vulpis in Daejeon, Korea. First stage larvae of C. vulpis were detected by fecal examination using the Baermann technique. Thoracic radiographs revealed mild, pervasive bronchial infiltration of the lung. Enumeration of larvae via the McMaster technique revealed 1,600 larvae per gram of feces. The dog was treated with mebendazole, and clinical symptoms were resolved 2 weeks post-treatment, as indicated by the subject presenting fecal tests negative for C. vulpis.
Animals
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Dogs*
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Feces
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Humans
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Korea
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Larva
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Lung
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Male
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Mebendazole
3.A Horsehair Worm, Gordius sp. (Nematomorpha: Gordiida), Passed in a Canine Feces.
Eui Ju HONG ; Cheolho SIM ; Joon Seok CHAE ; Hyeon Cheol KIM ; Jinho PARK ; Kyoung Seong CHOI ; Do Hyeon YU ; Jae Gyu YOO ; Bae Keun PARK
The Korean Journal of Parasitology 2015;53(6):719-724
Nematomorpha, horsehair or Gordian worms, include about 300 freshwater species in 22 genera (Gordiida) and 5 marine species in 1 marine genus (Nectonema). They are parasitic in arthropods during their juvenile stage. In the present study, the used gordian worm was found in the feces of a dog (5-month old, male) in July 2014. Following the worm analysis using light and scanning electron microscopes, the morphological classification was re-evaluated with molecular analysis. The worm was determined to be a male worm having a bi-lobed tail and had male gonads in cross sections. It was identified as Gordius sp. (Nematomorpha: Gordiidae) based on the characteristic morphologies of cross sections and areole on the cuticle. DNA analysis on 18S rRNA partial sequence arrangements was also carried out, and the gordiid worm was assumed to be close to the genus Gordius based on a phylogenic tree analysis.
Animals
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Dog Diseases/diagnosis/*parasitology
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Dogs
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Feces/*parasitology
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Helminthiasis, Animal/diagnosis/*parasitology
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Helminths/classification/genetics/*isolation & purification
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Male
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Molecular Sequence Data
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Phylogeny
4.Urogonimus turdi (Digenea: Leucochloridiidae) from the White’s Thrush, Zoothera aurea, in the Republic of Korea
Hyeon Cheol KIM ; Eui Ju HONG ; Si Yun RYU ; Jinho PARK ; Do Hyeon YU ; Joon Seok CHAE ; Kyoung Seong CHOI ; Cheolho SIM ; Bae Keun PARK
The Korean Journal of Parasitology 2019;57(5):461-467
Avian trematodes, Urogonimus turdi (Digenea: Leucochloridiidae), were collected from the intestine of wild birds, Zoothera aurea, 2013–2017 in the Daejeon Metropolitan City, Korea. The body was ellipsoidal, attenuated and/or round ends, 1,987–2,120 long and 819–831 μm wide. The oral sucker was subterminal, rounded anteriorly, and 308–425×351–432 μm in size; the prepharynx and esophagus were almost lacking; pharynx was well-developed, 142–179×78–170 μm in size; intestine narrow, bifurcating just after pharynx, ascending to the oral sucker before looping posteriorly and terminating near the posterior end; ventral sucker larger, in almost median, 536–673×447–605 μm and approximately 1.5 times larger than oral sucker. A phylogenetic tree constructed with 18S ribosomal RNA showed inter- and intraspecific relationships. Based on these morphological and molecular findings, we report here a U. turdi from White’s thrushes in Korea.
Birds
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Candidiasis, Oral
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Esophagus
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Intestines
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Korea
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Pharynx
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Republic of Korea
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RNA, Ribosomal, 18S
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Songbirds
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Trees
5.Detection and Identification of Sarcocystis cruzi (Protozoa: Apicomplexa) by Molecular and Ultrastructural Studies in Naturally Infected Korean Cattle (Bos taurus coreanae) from Daejeon, Korea
Tong Il CHOI ; Eui Ju HONG ; Si Yun RYU ; Cheolho SIM ; Joon Seok CHAE ; Hyeon Cheol KIM ; Jinho PARK ; Kyoung Seong CHOI ; Do Hyeon YU ; Jae Gyu YOO ; Bae Keun PARK
The Korean Journal of Parasitology 2018;56(2):121-127
To survey the prevalence of Sarcocystis infections, 210 heart samples were collected from Korean native cattle (Bos taurus coreanae) at an abattoir in Daejeon Metropolitan City, Republic of Korea. Sarcocysts were detected form 31 specimens (14.8%) and identified as Sarcocystis cruzi via transmission electron microscopy. The wall of S. cruzi has flattened protrusions that did not contain fibrils or microfilaments. The protrusions arose irregularly from the base, contained a fine granular substance, lacked internal microfilaments, and measured approximately 0.21–1.25 μm in length and 0.05–0.07 μm in width. Sequence analysis revealed 99.5% homology to S. cruzi. This is the first report on the prevalence of S. cruzi in native cattle from the Republic of Korea.
Abattoirs
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Actin Cytoskeleton
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Animals
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Cattle
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Heart
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Korea
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Microscopy, Electron, Transmission
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Prevalence
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Republic of Korea
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Sarcocystis
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Sequence Analysis