1.Epidemiological survey on a family aggregation COVID-19 in Y County, Chenzhou City, Hunan
Hong ZHOU ; Han wu ZHU ; Bai tang CHEN ; Wen HENG Z ; De biao HENG HE ; Jian HENG HUANG ; Shao feng HENG OUYANG ; Jun xiao HENG HUANG ; Hui HENG TAN
Shanghai Journal of Preventive Medicine 2020;32(2):E009-E009
Objective To investigate epidemic characteristics of a family aggregation COVID-19, and to provide scientific basis for prevention and control of family aggregation epidemic. Methods] Field epidemiological methods were used to investigate the cases and close contacts of a family aggregation COVID-19 in Y County, Chenzhou City, Hunan Province. Descriptive statistical analysis was used on epidemiological data . The 2019-nCoV nucleic acid was detected by real-time fluorescence quantitative RT-PCR. Results It was found that Ms. Deng was infected with COVID-19 and became the infectious source of the family aggregation epidemic , who had lived in Wuhan Hubei Province. Her boyfriend Mr. Cao became a second-generation case of COVID-19..Another two asymptomatic but infected persons were family members living with Ms.Deng . Conclusion COVID-19 easily spreads within families. The awareness of family members' protection, the education of new coronavirus pneumonia prevention and control in key groups should be strengthened to avoid the occurrence and spread of family aggregation epidemic.
2.Analysis of epidemic characteristics of COVID-19 clusters in Chenzhou, Hunan Province
Wei LIU ; Xun LIU ; Hong ZHOU ; Ying-xia LI ; Pin LI ; Wei-hua CHEN ; Wen ZHENG ; Han-wu ZHU ; Hui TAN ; Ya-meng HU
Shanghai Journal of Preventive Medicine 2021;33(11):1026-1030
Objective:To analyze the epidemiological characteristics of 8 clusters of coronavirus disease 2019 (COVID-19) in Chenzhou City, and provide scientific basis for epidemic prevention and control. Methods:Descriptive epidemiological analysis was conducted for 8 COVID-19 clusters, comparing and analyzing the differences of infection rates among close contacts within and outside the family, and emphatically describing two typical cases. Results:8 COVID-19 clusters were reported in Chenzhou with a total of 31 cases from January to February, 2020. The main source of infection of the family index cases was Hubei Province. Cough symptoms were observed in 67.74% of the cases, followed by fever (54.84%). The infection rate of close contacts within the family (55.00%) was higher than that outside the family (2.56%), and the difference was statistically significant (
3.Epidemiological characteristics of imported malaria in Chenzhou City, Hunan Province from 2010 to 2017
Han-Wu ZHU ; Yan-Qin WANG ; Hui TAN
Chinese Journal of Schistosomiasis Control 2019;31(2):182-184
Objective To study the epidemiological characteristics of imported malaria in Chenzhou City, Hunan Province, so as to provide the reference for consolidating the malaria elimination and formulating the prevention and control strategies of imported malaria. Methods The epidemiological characteristics of imported malaria were statistically analyzed in Chenzhou City from 2010 to 2017. Results Totally 46 malaria cases, which were all imported, were reported in Chenzhou City from 2010 to 2017, with an average annual incidence of 0.12/105. The reported malaria cases were mainly falciparum malaria, accounting for 60.87% of the total number of cases. There was no obvious seasonal distribution of malaria cases, but the top of reported cases were in June. Totally 73.91% of malaria cases were concentrated in Beihu District, Suxian District, Guiyang County and Zixing City. These cases were mainly the young and middle-aged and 69.57% of the cases were from 36 to 60 years old. There was a statistically significant difference in the distribution of malaria patients among the age groups (χ2 = 47.80, P < 0.01). The median time from onset to diagnosis was 6 days, and the case confirmed institutions were dominated by municipal and above medical institutions, accounting for 52.17% of the total number of cases. There was a statistically significant difference in the proportion of confirmed cases among medical and health institutions at all levels ( χ2 = 41.96, P < 0.01). Conclusions The importation of malaria in Chenzhou City is still severe. The awareness of malaria diagnosis and treatment in primary medical institutions, malaria patients' serum tests, and the health education of malaria control and prevention knowledge should be strengthened to consolidate the malaria elimination results.
4.Clinical and genetic analysis of a patient with Desminopathy manifesting initially with myalgia after lower limb activity.
Jibao WU ; Jiping YI ; Wenhua ZHU ; Dongyue YUE ; Bin CHEN
Chinese Journal of Medical Genetics 2024;41(1):96-100
OBJECTIVE:
To explore the clinical characteristics and genetic variant of a patient with desminopathy manifesting with atypical symptoms.
METHODS:
A patient who was admitted to the Department of Neurology of Jing'an District Central Hospital on February 24, 2021 was selected as the study subject. Clinical data, laboratory tests, muscle pathology, muscle magnetic resonance imaging (MRI) and genetic testing of the patient were retrospectively analyzed.
RESULTS:
The patient had developed myalgia after lower limb activity, and gradually developed asymmetrical muscle weakness and atrophy of the lower limbs. Cardiac examination revealed atrioventricular block and decreased left ventricular diastolic function. Muscle MRI showed that semitendinosus, sartorius, gracilis, fibula, gastronemius and supinator muscles were selectively involved at the early stage. Muscle biopsy confirmed pathological changes of desmin positive myofibrils. Genetic testing revealed that the patient has harbored a c.1024A>G (p.n342d) missense variant in exon 6 of the DES gene. Based on the guidelines from the American College of Medical Genetics and Genomics (ACMG), the variant was rated as likely pathogenic (PS4_moderate+PM2_supporting+PP3_moderate+PP1).
CONCLUSION
Desmin disease has a great clinical heterogeneity. Postexercise myalgia of lower limbs is a rare clinical phenotype. For patients harboring the c.1024A>G (p.n342d) variant of the DES gene, in addition to semitendinosus and fibula, Cardiac involvement is relatively insidious and easy to be ignored in clinic. Timely muscle MRI, muscle biopsy and gene detection will help the early diagnosis of the disease.
Humans
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Myalgia/genetics*
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Desmin/genetics*
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Retrospective Studies
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Muscle, Skeletal
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Lower Extremity
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Mutation
5.Expression of hypoxia-inducible factor-1 alpha and vascular endothelial growth factor in malignant melanoma.
Yong CHENG ; Yan LUO ; Yong-sheng ZHOU ; Hai-yan WU ; Zhi-bin XIE
Chinese Journal of Plastic Surgery 2009;25(2):134-136
OBJECTIVETo evaluate the expression of hypoxia-inducible factor-1 alpha (HIF-1 alpha) and vascular endothelial growth factor (VEGF) and their relationship in malignant melanoma.
METHODS32 cases of malignant melanoma and 11 samples of normal skin were examined for HIF-1 alpha and VEGF expression by immunohistochemistry.
RESULTSThe expression rate of HIF-1 alpha and VEGF was 62.5% (20/32) and 84.8% (27/32), respectively, in malignant melanoma, and 9.1% (1/11) and 18.2% (2/11), respectively, in normal skin, showing a significant difference between the two groups. Positive relationship between the expression of HIF-1 alpha and VEGF was found in malignant melanoma (P < 0.05).
CONCLUSIONS1) The high expression of HIF-1 alpha and VEGF in malignant melanoma indicates that it may play an important role in the generation and development of malignant melanoma. 2) The positive correlation between the expression of HIF-1 alpha and VEGF in malignant melanoma suggests that HIF-1 alpha may be involved in the regulation of VEGF in malignant melanoma.
Adult ; Aged ; Case-Control Studies ; Female ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit ; metabolism ; Male ; Melanoma ; metabolism ; pathology ; Middle Aged ; Skin Neoplasms ; metabolism ; pathology ; Vascular Endothelial Growth Factor A ; metabolism
6.Application of serum antibody detection in the identification of transmission chain of COVID-19
Han-wu ZHU ; Jing LIU ; De-biao HE ; Hong ZHOU ; Wen ZHENG ; Liang ZHOU ; Hui TAN
Shanghai Journal of Preventive Medicine 2021;33(3):192-
Objective To explore the transmission chain of COVID-19 by serum antibody detection, and to provide scientific evidence for the prevention and control of the epidemic. Methods Field epidemiological investigation was used to determine the COVID-19 cases and their close contacts. The 2019-nCoV nucleic acid in throat swabs and anal swabs were examined by RT-PCR. Serum specimens were collected for anti-2019-nCoV IgM antibody detection and combined IgM/IgG detection. Results Case A had no confirmed exposure to COVID-19. However, case C and D had dinner and lived together with case A; they reported contact history and dinner history with other confirmed COVID-19 cases(H, L). Case A tested positive for 2019-nCoV nucleic acid, whereas case C and D were negative. Moreover, case A and C were IgM antibody positive, while case D was negative. Case A, C and D were all positive for combined IgM/IgG. In addition, case D had clinical symptom, while case C did not. Conclusion Serum antibody detection can be used as an effective supplement to the inference of transmission chain of COVID-19, which may facilitate determining the source of infection and improving the evidence.
7.Absorbable collagen membrane for secondary alveolar bone grafting in alveolar cleft surgery:safety and effectiveness
Chenzhou WU ; Weiyi PAN ; Chong FENG ; Zexi DUAN ; Zhifei SU ; Chunjie LI
Chinese Journal of Tissue Engineering Research 2015;(38):6223-6227
BACKGROUND:Absorbable colagen membrane can be theoreticaly applied to secondary alveolar bone grafting in alveolar cleft surgery, which can improve the bone preservation and slow bone resorption. However, there is stil no unified conclusion.
OBJECTIVE:To assess the efficacy and safety of absorbable colagen membrane for secondary alveolar bone grafting viaa systematic review.
METHODS:MEDLINE, EMBASE, CBM and CAJD were searched for eligible articles addressing clinical randomized controled or controled trials of absorbable colagen membrane for secondary alveolar bone grafting. Test group received bone grafting with absorbable colagen membrane and control group only received bone grafting. Meta-analysis on the clinical success rate of bone grafting and incidence of complications in the recipient region was delivered with Revman 5.3.
RESULTS AND CONCLUSION:Five clinical trials, involving 416 cleft sites and 387 participants, were included. Two had high risk of bias and the rest had unclear risk of bias. If “the height of new bone is≥ 50% of alveolar height” was adopted as clinical success, the clinical success rate of the test group was significantly higher than that of the control group (P=0.002, relative risk value=1.33, 95% confidence interval [1.11, 1.60]). If “the height of new bone is≥ 75% of alveolar height” was chosen as clinical success, the clinical success rate of the test group was higher than that of the control group, but there was no significant difference between the two groups (P=0.06, relative risk value=1.40, 95% confidence interval [0.99, 1.99]). For safety, the use of absorbable colagen membrane could not increase the complications incidence (P=0.35, relative risk value=0.66, 95% confidence interval [0.28, 1.58]). So, the use of absorbable colagen membrane is safe to improve the clinical success rate of secondary alveolar bone grafting in alveolar cleft surgery. More randomized controled trials should be considered to reinforce the conclusion.
8.The clinical application of absorbable internal fixation system in maxillofacial fractures:systematic review and Meta-analysis
Hanghang LIU ; Chenzhou WU ; Weiyi PAN ; Zhifei SU ; Zexi DUAN ; Long SHI ; Chunjie LI
Chinese Journal of Tissue Engineering Research 2015;(52):8509-8516
BACKGROUND:There are numerous clinical studies on comparing absorbable internal fixation system and titanium metal internal fixation system in maxilofacial fractures; however, the systematic reviews and Meta-analysis in this field are rare. OBJECTIVE:To compare the clinical effect of absorbable internal fixation system and titanium internal fixation system in maxilofacial fractures through systematic review and Meta-analysis. MATERIALS AND METHODS:The randomized controled trials and controled clinical trials regarding the application of absorbable internal fixation system and titanium internal fixation system in maxilofacial fractures were electronicaly retrieved from Medline, Embase, the Cochrane Central Register of Controled Trials, China Biology Medicine disc, and China Academic Journal Network Publishing Database using the keywords. Meta-analysis was conducted using Revman 5.3 software. RESULTS AND CONCLUSION:Thirteen clinical studies were included, and totaly 1 718 patients were involved. The Meta-analysis results showed that the removal rate of implants in the absorbable internal fixation system group was significantly lower than that in the titanium internal fixation group (P=0.000 2); there were no significant differences in the healing rate of fracture I stage, the incidence of insufficient fixation in fracture site and the incidence of long-term complications between these two groups. These results demonstrate that the efficiency and safety of absorbable internal fixation system in maxillofacial fracture is satisfactory, and can reduce the proportion of secondary surgical removal of the implant. More randomized controlled trials should be conducted to confirm this conclusion.
10.Clinical characteristics of bocavirus infection among children.
Jin-song LIU ; Qiao-tu LIU ; Jin-liang TAN ; Ji-juan HE ; Wei-ping WU ; Wen-xiong LUO ; Xiao-wang QU ; Zheng-yu QI ; Zhao-jun DUAN
Chinese Journal of Experimental and Clinical Virology 2007;21(2):141-143
OBJECTIVETo study the clinical characteristics of human bocavirus (HBoV) among children and to understand the association of HBoV with human diseases.
METHODTotally 148 nasopharyngeal aspirate (NPA) samples were collect from hospitalized children with acute respiratory infection during Oct. 2005 to Feb. 2006. Two serum samples were obtained from HBoV positive patients. PCR was used to assay all these samples and PCR products were sequenced.
RESULTHBoV was positive in 11 of 148 NPA samples. The positive rate was 7.4 percent. The serum samples of HBoV infected patients showed that serum contained HBoV by PCR assay. All these HBoV positive patients had the clinical symptoms of bronchitis, bronchopneumonia and pneumonia. Some patients had diarrhea.
CONCLUSIONAll patients infected with HBoV had upper and lower respiratory tract infections. HBoV is a probable important pathogen of upper and lower respiratory tract infection. The HBoV could cause viremia. In addition, some HBoV patients had diarrhea. HBoV infection probably could also result in intestinal disease and other related symptoms.
Bocavirus ; genetics ; isolation & purification ; Child ; Child, Preschool ; DNA, Viral ; blood ; Female ; Humans ; Infant ; Male ; Parvoviridae Infections ; virology ; Respiratory Tract Infections ; virology