Objective:To observe the changes of cerebrospinal-fluid albumin index and blood-brain barrier in patients with central nervous system infection. Methods:The levels of albumin in serum and CSF were determined by using immunoturbidimeter method in 18 patients with tuberculous meningitis(TBM) in acute phase and recovery phase,23 patients with viral encephalitis(VE) in acute phase, and 18 normal people. Results:In acute phase, the levels of CSF albumin and CSF albumin index in patients with TBM and VE were obviously higher than those of control group(P

ObjectiveTo investigate the perioperative nursing experience in patients with renal cell carcinoma treated by cryoablation.Method The clinical data of 34 patients with renal cell carcinoma treated by cryoablation were retrospectively analyzed to summarize the perioperative nursing strategies.Results All the patients lived through the operations.The complications after the surgery occurred in 7 cases,with bleeding in 4 cases,urinary fistula in 1 case and wound infections in 2 cases.All the complications were cured by active treatment.After following up for 6 months,all the tumors showed liquefaction and necrosis and no one relapse. Conclusion Active and effective nursing intervention is the key point for the success of cryoablation.

Objective To evaluate the incidence of cancer in children born with assisted reproductive technology (ART ) . Methods The literatures were obtained by computer through EBSCO full‐text database ,Pubmed ,Germany Springer journal data‐base ,Cochrane Libarary ,Chinese academic journals published in the pool of network (CNKI) ,Wanfang and Veipu Chinese Journal Full‐text databases ,and other search methods .ART and child health natural pregnancy cohort study were included .The Meta‐anal‐ysis was conducted by using RevMan5 .0 software .Results Sixteen studies were included according to the criteria ,and 89 894 cases of ART‐born offspring were collected .The results of Meta analysis revealed that there was no significant difference between ART and non‐ART children on the incidence of tumor (OR= 1 .85 ,P> 0 .05) .Conclusion This study showed that the probability of ART offspring suffering from malignant is basically the same with non‐ART children ,but the potential long‐term risks are not yet clear .

Melanoma is a highly aggressive malignant skin tumor characterized by rapid growth and early metastasis. Surgical resection is currently still the main treatment. There are still some challenges in the early diagnosis and thorough surgical resection of melanoma, which affect the prognosis of patients. To date, great progress has been made in the application of fluorescent probes to the diagnosis and surgical resection of, as well as sentinel lymph node localization and resection in melanoma. Fluorescent probes have advantages of good chemical stability, less adverse effects, high specificity, high accuracy and visualization, and may provid new ideas for the diagnosis and treatment of melanoma.

Background and purpose:The impact of the two factors of von Willnbrang factor(vWF) and integrin?3 on tumor metastasis has not been recognized. This study was done to investigate the expression of vWF and integrin?3 in human lung cancer cell line H460,the association between the two factors and tumor metastasis effect of vWF and integrin?3 on adhesion of human lung cancer cell line H460. Methods:The expression of vWF and integrin ?3 protein was examined by immunohistochemical staining. The impact of vWF and integrin?3 on adherent effect of tumor cells was evaluated by adhesion experiment, antibody inhibiting experiment and MTT.Results:Positive expression of vWF and integrin?3 was detected in H460 human lung cancer cells. H460 human lung cancer cells were able to adhere to vWF. Using anti-integrin?3, we observed that the ability of cell adhesion to vWF was inhibited,and A Value decreased from 1.59?0.06 to 0.55?0.03(P=0.01619). Using anti-vWF, we observed cell adhesion to vWF was inhibited too and A Value decreased from 1.60?0.06 to 0.54?0.03(P=0.01598),which had the same effect when using anti-integrin?3.Conclusions:H460 human lung cancer cells are capable of producing vWF, and vWF expression contributes to metastasis by adhering to cancer cells, integrin?3 is the vWF receptor on H460 human lung cancer cells.

Objective To investigate the vaginal microbilme characterization in women with human papillomavirus(HPV)16 and 18 infection and high-grade cervical intraepithelial neoplasia(CIN).Methods Three groups were set,including control group(without HPV infection and with colpitis),low-grade CIN group(with HPV 16 or/and 18 infection),and high-grade CIN group(with HPV 16 or/and 18 infection).Virginal microbial composition was determined by using 16S rRNA amplication sequencing and hierarchical clustering analysis among three groups was applied.Rank sum test was used to determine statistical significance.Results Diversity and complexity of bacteria was significantly elevated in high-grade group.Compared with the control,the growth of Lactobacillus crispatus(L.crispatus) and Lactobacillus casei(L.casei) was significantly decreased,while Lactobacillus iners(L.iners) was significantly increased in low-grade CIN group and high-grade CIN group,and both were proportionally correlated to the grade of the disease.Brucella canis(B.canis),as colonized bacteria,was significantly decreased in both low-grade and high-grade CIN groups,and the changes were proportional to the grade of disease.The growth of Dialistersuccinatiphilus(D.succinatiphilus),Gardnerella vaginalis(G.vaginalis) and Prevotellabivia(P.bivia) were significantly elevated and proportional to the grade of disease.Conclusion The diversity and complexity of virginal microbial composition could be significantly expanded in women with HPV infection and high-grade CIN.In contrast to the important role of lactobacillus in virginal health,L.inners could be pathogenic.The colonization of B.canis,D.succinatiphilus,G.vaginalis and P.bivia might be highly correlated with HPV chronic infection and further development of CIN.

The treatment of renal cacinoma with inferior vena cava tumor thrombus is priority to the surgery,because of serious tranma and high risk the traditional operative method.Surgical procedure has been developed and changes.The development and application of vascular intervcntional technique make operation more safe and convenient.This article summarizes the application of renal artery intervention,inferior vena cava filter,balloons and vascular endoscope in the treatment of renal carcinoma with inferior vena cave tumor thrombus.

Objective To investigate the clinical characteristics and surgical management of adult presacral tumors.Methods The clinical data of 24 patients with adult presacral tumors from 2007 to 2012 were retrospectively analysed.All patients were diagnosed by digital rectal examination and imaging examination before operation.Seventeen patients with via sacrococcygeal approach,4 patients with via abdominal approach,and 3 patients with via combined abdominal and sacrococcygeal approach.Results The incision of 22 patients primary healing.There were 2 patients with postoperative incision infections,after dressing change cure.No patients died during perioperative period.Twenty patients were followed up for 3-17 months,1 patient with via abdominal approach recurrenced,and was healed after a second surgical resection.Conclusions Digital rectal examination and imaging examination are the main methods for diagnosis of adult presacral tumors.Most adult presacral tumors can be resected through sacrococcygeal route.

Objective To investigate the results of tumor specific growth factor(TSGF) levels in in healthy people and cancer patients in Guangzhou areas .Methods The serum TSGF levels of 6 615 health check-up receivers and 287 patients with cancer in the hospital from July 2012 to June 2013 were detected .Results In health check-up receivers ,the level and positive rate of TSGF were higher in >40-50 age women .The level and positive rate of TSGF in men were increased with age .The levels of TSGF in pa-tients with lung cancer ,liver cancer and digestive system tumor were higher than that in health check-up receivers(P<0 .01) ,but there has no statistically difference between genital system cancer patients and healthy check-up receivers(P>0 .05) .Conclusion Performing TSGF test in health examination is great significance to the early diagnosis of malignant tumors .The reference value range of TSGF should be set according to different gender and age .

Objective To screen proteins from human pancreas cDNA library,which interact with hepatitis C virus(HCV)E1 protein.Methods The human pancreas cDNA library was amplified,purified and evaluated,and then the purified library plasmids were transformed into yeast strain Y187.The reconstructed plasmid pGBKT7-E1 was transformed into yeast strain AH109 and screened on the nutrient deficiency medium SD/-Trp.The transformed AH109 mated with Y187 that contained the library plasmids.The diploid yeast cells were plated on nutrient deficiency medium SD/-Trp/-Leu/-His/-Ade and SD/-Trp/-Leu/-His/-Ade containing X-α-gal for selecting.The plasmids in diploid yeast cells were extracted and electrotransformed into E.coli DH5α.The plasmids in DH5α were extracted,sequenced and blasted.Result Sixteen proteins interacting with HCV E1 were found.Conclusion Some of the sixteen pancreatic proteins may be related with metabolisms of glucose and lipid.