Objective:This study aimed to validate the accuracy and validity of Her-2 assessment used for endoscopic biopsies and lymph node metastases by comparing these samples with the surgical materials obtained from the same patients. This study also aimed to analyze the relationship between primary tumor and clinicopathological characteristics of gastric cancer. Methods:Her-2 sta-tus was evaluated according to Gastric cancer Her-2 testing guidelines in 107 cases of gastric cancers with coupled endoscopic biopsies and surgical materials and in 76 cases of the corresponding lymph node metastases. Results were then compared and analyzed. Re-sults:The complete concordance of the Her-2 status of endoscopic biopsies and surgical samples (n=107) was noted in 86.9%of the cases. This result is consistent between the two different samples (Z=6.381 3, P<0.000 1). Primary gastric cancer and the corresponding lymph node metastases also displayed uniformity (Z=3.027 4, P=0.002 5). The positive rate of Her-2 was significantly associated with Lauren type, tumor histological grade, depth of invasion, lymph node metastasis, and TNM stage. Conclusion:A high concordance was observed between primary tumor and biopsy specimen (endoscopic biopsies and lymph node metastases), in which Her-2 status exhibit-ed a high predictive value. Her-2 status is correlated with invasion and cancer metastasis. Hence, the most appropriate criteria for Her-2 status assessment in lymph nodes should be considered to improve the Her-2 diagnosis system of gastric cancer.

Objective:To investigate the protective effect of dagglitazine on cardiovascular and renal function in patients with type 2 diabetes mellitus.Methods:A total of 200 patients with type 2 diabetes patients with poor blood glucose control who received treatment in Huxi Hospital Affiliated to Jining Medical University (Shanxian Central Hospital) from January to December 2019 were included in this study. They were randomly assigned to receive treatment with either metformin ( n = 100, control group) or dagglitazine ( n = 100, observation group) based on diet control and exercise therapy for 12 weeks. Before and after treatment, blood glucose, blood lipid, blood pressure, body mass index, cardiac function and renal function were compared between the control and observation groups. Adverse reactions were also monitored in each group. Results:After treatment, blood glucose, blood lipid, body mass index, cardiac function and renal function in each group were improved compared with before treatment (all P < 0.05). Fasting blood glucose, 2-h postprandial blood glucose, glycosylated hemoglobin, systolic blood pressure, diastolic blood pressure, total cholesterol, triglyceride, low density lipoprotein cholesterol, body mass index, left ventricular end diastolic diameter, serum creatinine, uric acid and cystatin C in the observation group were (6.48 ± 0.72) mmol/L, (8.03 ± 0.77) mmol/L, (6.16 ± 0.63)%, (126.03 ± 3.86) mmHg, (75.62 ± 2.87) mmHg, (3.83 ± 0.17) mmol/L, (2.21 ± 0.36) mmol/L, (2.34 ± 0.15) mmol/L, (23.03 ± 0.55) kg/m 2, (52.10 ± 2.13) mm, (39.97 ± 1.62) μmol/L, (237.17 ± 20.34) μmol/L, (0.64 ± 0.06) mg/L, respectively, which were significantly lower than (8.01 ± 0.84) mmol/L, (10.03 ± 0.90) mmol/L, (7.30 ± 0.72)%, (130.06 ± 4.79) mmHg, (79.60 ± 3.19) mmHg, (4.67 ± 0.37) mmol/L, (2.51 ± 0.57) mmol/L, (2.74 ± 0.19) mmol/L, (24.03 ± 0.60) kg/m 2, (57.22 ± 1.74) mm, (80.00 ± 6.88) μmol/L, (281.62 ± 40.52) μmol/L, (0.76 ± 0.09) mg/L, t = 13.850, 16.866, 11.933, 6.549, 9.263, 20.879, 4.469, 16.982, 12.410, 18.634, 10.626, 9.804, 18.876, all P < 0.001). After treatment, high density lipoprotein cholesterol, left ventricular ejection fraction, and the average distance walked by patients during the 6-min walk test in the observation group were (2.47 ± 0.15) mmol/L, (39.97 ± 1.62)%, (366.50 ± 17.74) m, which were significantly superior to those in the control group [(1.70 ± 0.20) mmol/L, (36.77 ± 1.21)%, (323.70 ± 12.48) m, t = 30.255, 16.435, 19.733, all P < 0.001). There was no significant difference in the incidence of adverse reactions between the two groups ( P > 0.05). Conclusion:Dagglitazine protects cardiovascular and renal function by reducing blood glucose, blood pressure, blood lipid, serum creatinine, serum uric acid levels and decreasing body mass index in patients with type 2 diabetes mellitus.

malathion.68 days after chlopyrifos was sprayed,the decrease rate of larvae density was still 95.34%.Conclusion The efficacy and persistent activity of chlopyrifos and the mixture of Bti and Bs-10 against mosquito larvae was better than the others.To control mosquito larvae in field,the suitable larvicides and application dosages should be applied according to the environment,characteristics,the pollute condition of water body etc.

Objective To study the possibility of umbilical cord blood mononuclear cells(UCB MNCs) having polydifferential potention to be induced and differentiated into hepatic stem/progenitor cells.Methods Collecting 32 cases fresh UCB from mature delivery,UCB MNCs were isolated by density-step centrifugal effect and were randomly divided into two groups:(1) control group 8 cases:the basic medium supplemented without cytokines;(2) trial group 24 cases,divided into four groups randomly,and the experimental results were detected at 0d,7d,14d,21d,respectively.The basic medium supplemented with various combinations of growth /differentiation factors(FGF-1,FGF-2,LIF,SCF,HGF,and OSM);cell morphological change was observed,hALBmRNA was examinated using RT-PCR methods,the expressions of ALB、CK18、CK19 were detected by immunohistochemistry.Results In trial group cell number increased,cell volume enlarged,refraction strong,polymorphic cells(mostly oval cells) appeared and obviously attached on the culture dishes.The expression of CK18、CK19、ALB were positive by immunohistochemistry,and the level of hALBmRNA improved along with the culture time.Conclusions UCB MNCs can be induced and differentiated into hepatic stem/progenitor cells in vitro.Human umbilical cord blood mononuclear cells may be a new source of transplantable hepatic stem/progenitor cells and hepatocytes.

Objective To evaluate the clinical results of patients submitted to laparoscopic assisted supracervical hysterectomy for benign uterine disease.Methods Women referred for supracervical hysterectomy for benign uterine diseases were divided into laparoscopy group(n=34)or open group(n=30).The operative time,blood loss,complications,morbidity,ileus,wound infections were compared between the two groups.Results In terms of Operative time,both of total operative time and oophosalpingorectomy time,there was no significant difference between laparoscopic group and open group.Less blood loss was found in laparoscopic group than open group.No complications were found in both groups.Conclusions Laparoscopic assisted supracervical hysterectomy is a safer and easier approach to complete supracervical hysterectomy with reduced blood loss and quick convalescence.It can be successfully integrated into a large health maintenance organization/residency-training program.

Objective To observe and explore the effectiveness of irbesartan hydrochlorothiazide(IH) combine with Bisoprol‐ol fumarate(BIS) on youth hypertension .Methods Randomly divided 96 patients in our hospital from September 2012 to February 2015 into observation group and control group(48 cases in each/group) .the IH treatment was given to the control group based on regular drug ,while the BIS was given to the observation group on the basis of the control group .systolic pressure(SBP) ,diastic pressure(DBP) and heart rate(HR) ,left ventricular end diastolic diameter(LVEDD) ,E peak and left ventricular ejection fraction (LVEF) in the two groups before and after treatment were detected ,and comprehensive efficacy were evaluated based on blood pressure improvement .Results Before treatment ,the difference of SBP ,DBP ,HR ,LVEDD ,E peak and LVEF between the two groups was not statistically significant (P>0 .05);after treatment ,SBP ,DBP and HR were (116 .4 ± 11 .8)mm Hg ,(85 .3 ± 6 .7) mm Hg and (65 .2 ± 7 .1)times/min in the observation group ,respectively ,while in the control group ,SBP ,DBP and HR were (132 .8 ± 14 .6)mm Hg ,(96 .3 ± 6 .2)mm Hg and (75 .2 ± 8 .1)times/min ,respectively ,the difference was statistically significant(P<0 .05);after treatment ,LVEDD in observation group was significantly lower than the control group ,while LVEF ,E peak were significantly higher(P< 0 .05);the total effective rate in observation group was 95 .8% (46/48) ,while total effective rate was 75 .0% (36/48) in the control group ,the difference was statistically significant(P<0 .05) .Conclusion IH combined with BIS can significantly improve blood pressure in patients with juvenile hypertension and has significant effect ,thus it is a safe and effective therapy for juvenile hypertension .

Large number of cystic fibrosis transmembrane conductance regulator (CFTR) gene mutations are responsible for a wide spectrum of disease expressions in CF (cystic fibrosis). They includs not only the malfunction of the lung and pancreas, intestinal obstruction, and incapable of salt reabsorption, but also some kinds of male infertility, such as congenital bilateral absence of the vas deferens (CBAVD),poor sperm quality, the obstruction and agenesis of the epididymis. More recent studies have indicated that CFTR gene mutations have a far-reaching effect on human reproduction.

Objective To investigate the radiosensitivity of stem cells in pancreatic adenocarcinoma PANC‐1 cell line and the possible mechanism of its radiation resistance .Methods Using flow cytometry ,the cells were isolated and sorted into CD44+CD24+ ,CD44-CD24+ ,CD44+CD24- ,and CD44-CD24- .Multi‐target click model was used to fit cell survival curves and deter‐mine the sensitizer enhancement ratio .The apoptosis and cycle distribution of the four cell subsets were determined using flow cy‐tometry ,and the level of ROS was determined by DCFH‐DA probe .Results The ratio of CD44+ and CD24+ in the sorted PANC‐1 cell line was 92 .0% and 4 .7% respectively .Before radiation ,there was no statistically significant difference between four groups (P>0 .05);After treated with 6MV‐X ray ,The ratio of apoptosis was the lowest in CD44+CD24+ (P<0 .01);The percentage G0/G1 cell was the highest in CD44+CD24+ (P<0 .01) ,the sensitizer enhancement ratio of CD44+ CD24- ,CD44-CD24+ and CD44-CD24- were 1 .61 ,1 .81 ,1 .94 ,respectively .The level of ROS in CD44+CD24+ was lower (P<0 .01) .Conclusion Tumor stem cells of pancreatic adenocarcinoma have properties of a lower level of ROS and relative stationary that maybe the reasons of radio resist‐ance .

In this study, a novel polyurethane membrane, modified by superfine silk-fibroin powder, was prepared for small-diameter vascular grafting. Scanning electron microscopy, transmission electron microscopy, and histological examination were applied to evaluate histocompatibility of this polyurethane membrane. The polyurethane membrane was compared with polytetrafluoroethylene material. A pseudomembrane and gap formed between polytetrafluoroethylene and the surrounding tissues, and no cells infiltrated or grew into the polytetrafluoroethylene material. On the contrary, superfine silk-fibroin powder/polyurethane blend membrane merged tightly with the surrounding tissues without gaps, and cells infiltrated and grew into the material. Moreover, the negative effects of superfine silk-fibroin powder/polyurethane blend membrane on cells were less than those of its polytetrafluoroethylene counterpart. Our findings indicated that the superfine silk-fibroin powder/polyurethane blend membrane has better histocompatibility than polytetrafluoroethylene membrane. It is concluded that the superfine silk-fibroin powder/polyurethane blend membrane is a promising biomaterial for small-diameter prosthesis.

Mild cognitive impairment is a transitional state between normal aging and dementia, which is the best intervention window to delay cognitive decline.As one of the adjustable risk factors affecting cognitive function, perceived stress significantly increases the risk of cognitive impairment by causing hypothalamic-pituitary-adrenal axis dysfunction and psychological disorders.Therefore, perceived stress management will become a new hot spot in the research and practice of non-drug intervention techniques for cognitive impairment.This paper reviews the current situation of perceived stress in MCI population, the relationship between perceived stress and cognitive function, and the evaluation techniques of MCI individual perceived stress.Further more, this paper summarizes the relevant influencing factors of perceived stress based on stress attribution model, and comments on the action mechanism and practical enlightenment of perceived stress intervention program on cognitive function management, in order to provide theoretical reference for cognitive function management of MCI population.