Objective To investigate whether the T cells (CD4+ CD25+) levels and the irregular antibodies screening cloud improve the diagnosis of invalid red blood cells transfusion.Methods Thirty-one patients with red blood cell invalid transfusion in the People's Hospital of Yubei District of Chongqing were selected.Flow cytometry was used to detect the changes of CD4+CD25+ regulatory T cells.Standard cells Ⅰ,Ⅱ,Ⅲ were used to screen on irregular antibodies in red blood cells (RBC).Results The rate of invalid RBC transfusion was 7.52％ (31/412) in Yubei District.The incidence rate of medical diseases was 77.42％ (24/31),much higher than surgical disease (22.58％ (7/31,P =0.002),and the major departments were oncology department,hematological department and infectious department.The CD4+ CD25+ regulatory T cells were decrease from (22.18±1.58) ％ to (16.57±1.77) ％(P=0.023).Conclusion Combin CD4+CD25+ regulatory T cells test and irregular antibody screening can help prevent and reduce the invalid transfusionof red blood cells.

Diabetic kidney disease is one of the main causes of end-stage renal disease .During the past decades , proteomic analysis based on mass spectrometry has been playing a momentous role in revealing potential biomarkers of diabetic kidney disease .Assays using New combination of multiple biomarkers can help identify high risk patients and give positive guidance of therapies .

Objective To investigate the effects of myricetin (Myr) on A549 lung cancer cell proliferation and its mechanism. Method The inhibitory effects of Myr on the growth of A549 cells were investigated and its value of IC50 after 72 h incubation was measured by 3-(4,5-dimethyl-thiazolyl -2)-2,5-diphenyl tetrazolium bromide (MTT) method. The phosphorylation level of Akt protein was studied by Western blotting. Results Myr inbibited A549 cell proliferation in dose-dependent way. The IC50 value of Myr treatment for 72 h was 41.7 ?g/ml. Treatments with 32 ?g/ml Myr for 12 h significantly reduced the protein kinase B (Akt Ser473) phosphorylation. Conclusion Myr could inhibit the growth of A549 cell in a dose-dependent way. Akt phosphorylation may be the mainly molecular target of Myr in A549.

lymphocytic thyroiditis.

Objective To establish a MRI grading diagnostic model for chronic pancreatitis (CP) to acquire better combination for CP diagnosis and improve the diagnostic ability of CP grading.Methods To collecte the data of 68 CP patients who were clinically diagnosed and 23 health volunteers from Dec 2012 to Oct 2014.There were 23 mild CP, 14 moderate CP and 31 severe CP according to Cambridge classification.3.0 T MRI data were analyzed, and 14 features were extracted to compare the MRI features among groups.The single ordinal regression analysis was conducted on the variables with significant difference between groups, and the collinearity was diagnosed.The factor analysis was used for multicollinearity.The multiple ordinal logistic regression was finally conducted to establish the regression model.Results There was no significant difference between control groups and CP groups on pancreas divisum (X14), but significant differences were found in other 13 features (P<0.05).Single ordinal regression analysis of 13 features showed that all features except pancreatic parenchymal bubble (X12) were significantly correlated with CP grading diagnosis(P<0.05).The effect of multicollinearity was validated among 9 continuous variables.Three common factors were identified, including F1(X3、X4、X5、X9、X8), F2(X7、X6) and F3 (X1、X2) which represented the exocrine function, the features of main pancreatic ducts and pancreatic parenchyma, respectively.Six features were implemented into the multi Logistic regression model, which included F1, F2, F3, X10 (the visualizations of branch pancreatic duct after secretin stimulation), X11 (pancreatic shape) and X13 (the filling defects of main pancreatic duct).Finally, the most appropriate regression model was gotten, which was the scale model of the probit link function.The model′s diagnostic accuracy for normal, mild CP, moderate CP, severe CP and total CP was 96.65%,100%, 71.42%,100% and 94.50%, respectively.Conclusions The ordinal logistic regression model proposed in this study may accurately predict the CP grades and can offer valuable references for clinic diagnosis and therapy of CP.

The complement system is a complex protein network.Complement is important in disease severity and response to therapy.This review summarizes recent advances on the role of complement analysis in complement function or activity,complement factors concentrations,complement activation products,complement autoantibody and genetic analysis.And it also focuses on pre-analytical and post-analytical quality control for complement analysis.

Objective To investigate the CT imaging features and its diagnostic value in patients with epibulbar dermoid and dermolipoma. Methods Twelve cases with epibulbar dermoids and dermolipomas were retrospectively analyzed. There were 5 dermoids and 7 dermolipomas, and a distinct female predominance (11/12). The lesions located at the external canthus, and were almost always uniocular and single (right=9, left=3). Transverse plain CT scan was performed in all patients, contrast enhanced CT scan in 5 cases. The clinical aspects, pathological features, and CT findings were then described. Results Crescent-shaped hypodensity lesion attached to the epibulbar was detected in all cases on CT scan. The diameter ranged from 8 mm?5 mm to 25 mm?15 mm. The lesion had a thin wall (1- 3 mm) under the conjunctiva lateral, and could have middle enhancement. Conclusion CT can show the lesion′s range and characteristics, as well as the relationship with the adjacent structures. CT has great directive value for operating on these tumors, and reducing the complications.

Objective To lay the foundation for further exploration on parasitifer's defence reaction to pneumolysin through constructing ply gene-deletion strain of Streptococcus pneumoniae and researching on its virulence change. Methods A linker fragment with erm gene in middle and homologous upstream and downstream fragment of ply gene at both sides was prepared by long flanking homology-polymerase chain reaction(LFH-PCR). The linker fragment was transformed into Streptococcus pneumoniae. ply-deficient strain was then screened out from blood plate which contains erythromycin and identified by PCR. ply-deficient strain growth in vitro was observed and virulence change was observed through infecting mouse model. Results PCR results showed that ply gene was replaced completely by erm gene. The ply deficient strain was successfully constructed. The growth of single strain culture medium showed that ply genetic defect made no influence on bacterial's external growth. While in the mice nasal cavity infecting experiment, deficient strain enter into blood after 6 h from infecting which obviously slower than that did wild-type(2 h). And the number of bacteria at each point was much smaller than that of wild-type(P ＜0. 01 ). The mice peritonaeum infecting experiment showed that median lethal time of wild-type was 3 d, while that of deficient strain was 18 d(P＜0. 01). Conclusion It is a good way to completely substitute ply gene using LFH-PCR. ply deletion made no influence on baterial's growth in vitro, but it resulting in reduction of bacterial virulence in vivo.

To summarize the nursing experience of 5 patients with severe ARDS complicated with hypercapnia who underwent extracorporeal carbon dioxide removal(ECCO2R).Key points of nursing care included:establishing a rapid emergency response nursing team for ECCO2R;PETCO2 real-time monitoring to grasp the timing of the machine;ensuring continuity of treatment and improving the removal efficiency;respiratory-related monitoring;prevention of complications of blood coagulation and hypothermia;and weaning from extracorporeal carbon dioxide removal.After careful treatment and care,all the 5 patients were successfully removed from ECCO2R treatment.

Objective: To analyze research hot spots and trends on myopia in children and adolescents in China, and to provide a reference for the future development of this research field. Methods: Using CiteSpace software, 725 eligible Chinese documents published over a 22 year period were sourced from CNKI, VIP and Wanfang Chinese databases, were analyzed during 2000-2021. The data were visualized by a keyword co occurrence map, and clustering, as well as burst and author cooperative networks. Results: The research field of myopia in children and adolescents in China had undergone three stages:an initial formation period, development period and rapid growth period, and it was expected that the annual number of papers will maintain an increasing trend in the future. "Myopia" "adolescents" "children" and "students" were the most frequent keywords(667, 535, 288, 47). The keyword burst "adolescents" started the earliest, and "students" had the largest burst strength and duration. Zhang Ning, Tang Wenting, Tao Fangbiao, Yang Xiao and others have made important contributions to this research field. Conclusion In recent years, the research focus in this field has gradually shifted toward the influencing factors, as well as prevention and treatment measures of child and adolescent myopia. The trend of myopia in young children and adolescents is obvious. In the future, research on the prevention, control and treatment of myopia in children and adolescents will become a hot spot.