1.Effect of mild hypothermia on β2-microglobulin level in cerebrospinal fluid of traumatic brain injury patients
Liqing LIN ; Xian FU ; Jiandong WU ; Qihua XIAO ; Surong QIAN ; Chenqiu WANG ; Jianren WANG ; Chen WANG
International Journal of Surgery 2011;38(11):742-744
Objective To study the effect of mild hypothermia on β2- microglobulin (MG) level in cerebrospinam(CSF) of traumatic brain injury (TBI) patients.Methods Thirty-six severe TBI patients were divided into two groups randomly,mild hypothermia treatment group and normathermia treatment group,and the β2- MG level and GOS score in CSF of these patients in different time- point were evaluated.Results β2-MG level increased at first,then decreased gradually,and MBP level in mild hypothermia group decreased greater than the control group( P < 0.05 ),moreover,patients in mild hypothermia treatment group had better outcome than the control group( P < 0.05).Conclusion Mild hypothermia may act as neuroprotection by inhibiting inflammatory response or improving immune regulation.
2.Effect of mild hypothermia on the MBP level in the CSF of TBI patients
Jianren WANG ; Xian FU ; Jiandong WU ; Qihua XIAO ; Surong QIAN ; Chenqiu WANG
Clinical Medicine of China 2012;28(2):152-154
Objective To study the effect of mild hypothermia on MBP level in the CSF of TBI patients.Methods We investigated 36 patients with severe traumatic brain injury and randomized them into two groups,mild hypothermia treatment group and normathermia treatment group.The MBP levels in CSF and ICP and GOS scores in different time-points were evaluated.Results There was no significant difference between the two groups in the MBP level on the 1st day after treatment(P > 0.05).The MBP levels in mild hypothermia group decreased greater than that in the control group on the 7th and 14th day after treatment(P < 0.05).ICP decreased more in mild hypothermia group than in the control group(P < 0.05)on the 7th and 14th day after treatment.Moreover,patients in mild hypothermia treatment group have better outcome than those in the control group(P < 0.05),but there was no significant difference in the death rate between the two groups.Conclusion Mild hypothermia may provide neuroprotection by reducing MBP degradation and stabilizing medullary sheath,thus improving the prognosis of TBI patients.
3.Ichthyosis vulgaris: a pedigree with 13 cases.
Chinese Journal of Medical Genetics 2017;34(3):397-397
Adult
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Child, Preschool
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Female
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Humans
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Ichthyosis Vulgaris
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genetics
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Infant
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Male
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Mutation
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Pedigree
4.Osteogenic effects of antihypertensive drug benidipine on mouse MC3T3-E1 cells in vitro.
Baixiang WANG ; Jiakang YANG ; Lijie FAN ; Yu WANG ; Chenqiu ZHANG ; Huiming WANG
Journal of Zhejiang University. Science. B 2021;22(5):410-420
Hypertension is a prevalent systemic disease in the elderly, who can suffer from several pathological skeletal conditions simultaneously, including osteoporosis. Benidipine (BD), which is widely used to treat hypertension, has been proved to have a beneficial effect on bone metabolism. In order to confirm the osteogenic effects of BD, we investigated its osteogenic function using mouse MC3T3-E1 preosteoblast cells in vitro. The proliferative ability of MC3T3-E1 cells was significantly associated with the concentration of BD, as measured by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and cell cycle assay. With BD treatment, the osteogenic differentiation and maturation of MC3T3-E1 cells were increased, as established by the alkaline phosphatase (ALP) activity test, matrix mineralized nodules formation, osteogenic genetic test, and protein expression analyses. Moreover, our data showed that the BMP2/Smad pathway could be the partial mechanism for the promotion of osteogenesis by BD, while BD might suppress the possible function of osteoclasts through the OPG/RANKL/RANK (receptor activator of nuclear factor-κB (NF-κB)) pathway. The hypothesis that BD bears a considerable potential in further research on its dual therapeutic effect on hypertensive patients with poor skeletal conditions was proved within the limitations of the present study.