1.Diterpene alkaloids from roots and processed products of Aconitum pendulum
Yujie WANG ; Chenjuan ZENG ; Zhe YAO ; Jing ZHANG ; Yi ZHANG ; Fan ZHANG
Chinese Traditional and Herbal Drugs 1994;0(03):-
Objective To study the diterpene alkaloids from the roots and processed products of Aconitum pendulum.Methods The chemical constituents were isolated and purified using silica gel column chromatography.Their structures were determined on the basis of NMR and mass spectra.ResultsTwelve compounds were isolated and identified as deoxyaconitine(1),3-acetylaconitine(2),aconitine(3),15?-OH-neoline(4),8-acetyl-15-hydroxyneoline(5),neoline(6),14-benzoyl-8-O-methylaconine(7),benzoylaconine(8),polyschistine D(9),benzoyldeoxyaconine(10),polyschistine A(11),and aconine(12).Conclusion Diterpene alkaloids are the main chemical constituents of A.pendulum and compounds 4—8 are found in this plant for the first time.Compared to raw materials,some boiled processed products change into new components:polyschistine D(9),benzoyldeoxyaconine(10),polyschistine A(11),and aconine(12).Identification of these components provides basis for the processing principles.
2.Testicular MCP-1 expression in mouse induced by systemic inflammation
Shaohong DING ; Gang CHEN ; Chenjuan YAO ; Junkang JIANG ; Chun WANG ; Yutaka NAKAHORI
Chinese Journal of Immunology 2009;25(12):1105-1107,1111
Objective:There is a large of population of macrophages resident in the testicular interstitial tissue under normal conditions and they are increased during inflammation.The mechanisms involved are unclear.This study focused on the expression of monocyte chenoattractant protein-1 (MCP-1) in the mouse testis before and after an intraperitoneal injection of LPS.Methods:The expression of MCP-1 in testis was detected by using reverse transcription-polymerase chain reaction and Western blot,the immunofluorescent technique was used to detect the localization of MCP-1 protein in testis.Results:In the normal testis,the expression of MCP-1 mRNA and protein was detectable by RT-PCR and immunofluorescent technique,respectively.The level of testicular MCP-1 mRNA increased dramatically at 3-24 h after LPS treatment,the level of MCP-1 protein increased at 12 h after LPS treatment.The MCP-1 was localized in the testicular interstitial tissue.Conclusion:MCP-1 may play a role in maintaining the resident macrophage population in normal testis and regulating monocyte and macrophage influx in inflammatory testis.
3.Study on processing principle of Aconitum pendulum.
Yujie WANG ; Jing ZHANG ; Huiping TIAN ; Chenjuan ZENG ; Zhe YAO ; Yi ZHANG
China Journal of Chinese Materia Medica 2010;35(5):588-592
OBJECTIVETo study the processing principles of different processed products of Aconitum pendulum.
METHODUsing high performance liquid chromatography and acute toxicity test to compare the changes in chemical composition and toxicity of the roots and processed products of A. pendulum.
RESULTThe main toxic components of the roots of A. pendulum were aconitine, deoxyaconitine and 3-acetylaconitine. The contents of these three alkaloids were significantly reduced in processed products, while benzoylaconitine significantly increased. In addition, processed products emerged aconine, polyschistine-D, beyzoyldeoxyaconine, 16-epi-pyroaconitine and 16-epi-pyrodeoxyaconitine. From the structural analysis, these new emerged compounds transformed from the aconitine, deoxyaconitine and 3-acetylaconitine.
CONCLUSIONDifferent processing methods can reduce the toxicity of the roots of A. pendulum. Processing principle is ester hydrolysis and high-temperature pyrolysis.
Aconitine ; analogs & derivatives ; analysis ; Aconitum ; chemistry ; toxicity ; Animals ; Female ; Male ; Mice