1.The analysis and thinking of stressors and mental health for postgraduates of military medical uni-versities
Chenhua LIANG ; Wenpeng CAI ; Jiao DONG ; Yonglin FENG
Chinese Journal of Medical Education Research 2016;15(9):889-893
Objective To determine the relationship between stressors and mental health among postgraduates in military medical universities. Methods This article analyzed population variable, mental health, the correlation of personality, coping styles and pressure based on Military Academy Graduate Student Stress Scale, Symptom Checklist-90, Simplified Coping Style Questionnaire and Eysenck Personality Questionnaire. Independent sample t-test and single factor analysis variance were used to compare the feel-ing of pressure differences among demographic variables groups and mental health differences between high and low pressure group. Pearson product-moment correlation and multiple step-wise regression analysis were performed to explore the relationship of personality, coping styles and feeling of pressure. Results ①There was significant difference on stressors among postgraduates in military medical universities (F=77.397, P=0.000), and LSD test showed that academic pressure> distribution pressure>economic pressure>marriage pressure>family pressure (P<0.05). ②Except phobic factors, SCL-90 total score and each dimension points of high stress group were significantly higher than that of low stress group (P<0.05).③Negative coping style and EPQ multiple dimensions significantly related to feeling of pressure values in post-graduate students. Neuroticism, lie and extroversion entered the regression equation (F=16.959,P=0.001) and together explained 19.0% of the total variance. Conclusion The pressure from teachers, academic, graduation distribution and application for jobs are the main source of pressure. And the students with high pressure sensitivity get poor mental health and tend to have neurotic personality traits and negative coping styles.
2.THE EXPERIMENTAL MORPHOLOGICAL OBSERVATION ON HAMSTER TRACHEA IN ORGAN CULTURE
Mingchang HE ; Shengming HONG ; Chenhua LIANG ; Jinming LIU ; Jianhua LI
Acta Anatomica Sinica 1953;0(01):-
This article deals with the organ culture on hamster trachea under the normal condition. The result showed that the epithelium of trachea could be kept alive up to 8 th-week at most by means of rotatory method of incubation. The culture media are RPMI 1640 (Nissui) and Eagle MEM (Nissui). Each medium was with or without bovine serum added. The results of histological, ultrastructural, radioautographic observations exhibited that the tracheal epithelia of hamster before culture showed ciliated columnar form with cilia. With prolongation of culture time, the changes of tracheal epithelium took the following order, from ciliated columnar to simple columnar and to simple squamous form. These changes were observed in all 4 groups. In radioautography, there are labeling granules in nuclei of tracheal epithelial cells in all 4 groups at different culture time, indicating that the epithelial cells still maintained DNA synthetic activity. TEM observation has showed ultrastructure of tracheal epithelium remained unchanged within 4 weeks. The ciliated cells are well with intact cilia. The intercellular junction is the tight one. Occasionally in accordance with LM observation a few of ciliated cell lacked cilia and its nucleus flattened. In cytoplasm, the dilatation of cisternae of rER and the incresing amount of ribosomes and lysosomes could be seen under TEM. During 7-8 weeks only a part of epithelia of trachea was still kept well, most of them became single layer. The ciliated cells lacked cilia, and their nuclei were long and oval in shape. Some enlarged lysosomes and autophagic vacuoles could be seen in cytoplasm indicating cytoplasmic degeneration. The present study suggested that both Eagle MEM and RPMF 1640 show the same culture effect.