ObjectiveTo discuss the feasibility and effect of clinical application of the single-bag and double-chamber anus tube.MethodsPatients who needed retention enema and non-retention enema(80 cases) were divided into 2 groups with 40 cases in each group.The test group was given enema by single-bag and double-chamber anus tube.The control group was given enema by traditional rubber anus tube.The reaction and clinical effect were compared between the two groups.ResultsIn the test group,pain and malaise lessened,intestinal cleaning was good,enema liquid reservation prolonged,the satisfaction degree of patients improved,compared with that of the control group (P<0.01).ConclusionsClinical application of self-made single-bag and double-chamber anus tube can give lesser pain and better effect of patients,and it improved satisfaction degree of patients and efficiency of nursing work greatly.

AIM: To develope a reversed-phase high performance liquid chromatographic method for the quantitative determination of chlorogenic acid and caffeic acid in Shegan Kangbingdu Injection. METHODS: The operation was carried out in the Kromasil ODS column with the mobile phase consisting of a mixture of water-methanol-acetonitril aceti acid(95∶10∶5∶2,v/v),in which the flow rate of 1.0 mL/min and UV detection wavelength at 326 nm were set to determine the contents of chlorogenic acid and caffeic acid. RESULTS: There were good linear relations between the concentrations and the peak-areas of chlorogenic acid and caffeic acid.The relative standard deviation of peak areas for chlorogenic acid or caffeic acid were 0.60%,0.32%,respectively.The two kinds of standard solutions were both stable in 16 h(RSD=0.54% for CGA,0.11% for CFA).The average recovery was 100.1%,99.6% for CGA,CFA,repectively.The detectable limit(S/N=3)was 0.012,0.020 mg/L,and the quantitative limit (S/N=10) was 0.037,0.052 mg/L,repectively. CONCLUSION:The method is simple,sensitive,rapid and accurate,and can be used for the quality control of Shegan Kangbingdu Injection

Objective To investigate the expression characteristics of miR-155 and miR-146a in mice with schistosomiasis and praziquantel(PZQ)treatment. Methods Totally 40 BABL/c mice were divided into 4 groups:a normal group,a 6W infect-ed group that were infected cutaneously with 10 Schistosoma japonicum cercariae for 6 weeks,a 12W infected group that were in-fected cutaneously with 10 Schistosoma japonicum cercariae for 12 weeks,and a praziquantel treated group that were infected cuta-neously with 10 Schistosoma japonicum cercariae and intragastrically administered with PZQ(300 mg/kg/day)for 1 day in 6 weeks post-infection and continuing surviving 6 weeks. The animals were sacrificed in 6 weeks and 12 weeks post-infection respectively. The left front lobe of each liver was stained with hematoxylin-eosin(HE)to detect pathological lesions. The levels of mRNA ex-pressions of miR-155,miR-146a and pro-inflammatory cytokines(TNF-α,IL-1βand IL-6)in the liver tissue were determined by using quantitative real-time PCR. Results The levels of mRNA expressions of miR-155,miR-146a and pro-inflammatory cyto-kines(TNF-α,IL-1βand IL-6)in the 6W infected mice were significantly higher than those of the normal mice and of the 12W in-fected mice. Compared with the 12W infected mice,the inflammation response of liver egg granuloma in the PZQ-treated mice was ameliorated. Furthermore,there was a marked increase in the levels of mRNA expressions of miR-155,miR-146a and three pro-in-flammatory cytokines in the PZQ-treated mice compared to the 12W infected mice. Conclusion miR-155 and miR-146a may play a role in schistosomiasis liver inflammation response and the inflammation regulation of praziquantel treatment.