Objective:To analyze the service pattern provided by online health community and the conversion of online health community to offline health systems. To put forward the problems and suggestions on integration of online and traditional health services. Methods: The crawl programming is programmed to acquire and analyze data from haodf. com website, including the online consultation, telephone counseling, and referral appointments, etc. Results:The results show that the average number of replies is 14 times for two weeks, the total reply is 418 times, the telephone answering rate is only 0 . 08%, and the referral rate to offline is 2 . 96%. Conclusion: The conversion rate of online health community in China is lower than e-commerce service. The recommended index of telephone consulting is more instructive to patients. The services mode provided by the online health community is still single, and the profit model is not even clear.

Objective:The online health community is a new business model which combines of the internet in-formation technology and the medical service. Through analyzing the development of the online health community in China, the author puts forward the policy recommendations to promote the rapid integration of internet technology and the traditional medical services. Methods:The crawler program of collecting data from haodf. com website is built up, and information was gathered from 166 hospitals in Beijing and 12,497 physicians' personal online platform informa-tion was analyzed. Data regarding the development of online health community were analyzed from the aspects of phy-sicians' activity, patients' visits and satisfaction, etc. Results:The information of hospitals and physicians provided by Haodf. com website was mainly gathered from public tertiary level hospitals. The professional titles of the physicians are mainly intermediate and deputy-senior ones. Only one-third of the physicians who opened the personal online pa-ges keep high activity and the patient satisfaction score was very high. Conclusion: The online health community in China has a certain scale and began to gradually change the traditional medical service system thereby altering the mode of medical treatment. However, its development prospects of important factors are based on the data usability and effectiveness of the online perform and deep using in health reform.

Objective: Developing New Rural Cooperative Medical System ( NCMS ) critical illness insurance is a relatively new operation mode for the government, which needs to provide quantify references for policy designers to help the predict decision. Methods: Through the analysis of decision process and behavior of the government and the commercial companies , the utility maximization functions for both the government and the commercial companies are set up based on the game theory. The Stackelberg model theory is then applied to figure out the relationship among different parameters in the utility maximization functions. Results and Conclusion: In the process of critical illness insurance under NCMS, the government should combine the local situation, to design reasonable polices based on the relationship among different parameters , so as to lead the NCMS critical illness insurance develop in a healthy way.

Objective To explore the current education situation and demand of network teaching for health service management course so as to provide recommendations for improvement.Methods Questionnaire survey was conducted among 232 undergraduates from majors of preventive medicine,stomatology and clinical medicine.Totally 204 valid questionnaires were organized and recorded into database and SPSS 12.0 was employed to make descriptive statistical analysis of the data.Statistical results were used to show the current education situation and demand of network teaching for health service management course.Results 52.94％ (108/204) of the students interviewed were dissatisfied with current teaching mode.87.25％ (178/204) of the students believed the course website would be beneficial for learning.For the construction of the course website,health insurance system,health policy analysis and health organization system were considered both important and difficult.59.31％ (121/204) of the students showed that the course website should be updated at least once a week.Conclusions It is necessary to explore the network teaching mode for health service management course.Teaching reform depending on different professions and curriculums should be carried out for network teaching.

Five compounds were isolated from the rhizome and roots of Rhodiola crenulata S.H.Fu.They werc identified as rhodionin (Ⅰ), rhodiosin(Ⅱ), tyrosol(Ⅲ), salidroside(Ⅳ) and gallic acid (Ⅴ), respectively, by UV, MS, 1H and 13CNMR spectroscopic and chemical reactions.

Objective:To investigate the relationship between helicobacter pylori ( Hp) infection and proliferation as well as invasion of Uygur esophagus squamous cell carcinoma.Methods: Immunohistochemistry were used to detect Hp , Ki67 and MMP2 protein in 164 cases of Xinjiang Uygur esophageal squamous cell carcinoma tissue specimens.Results: The positive rates of Hp,Ki67 and MMP2 were 81.7%(134/164),67.1%(110/54) and 86.6%(142/164) respectively in 164 cases of esophageal squamous cell carcinoma.The expression of Ki67 in the Hp positive group of patients with esophageal squamous cell carcinoma were significantly higher than that in the Hp negative group and had positive correlation between them (rs=0.340,P<0.01),the expression of MMP2 in the Hp positive group were significantly higher than that in the Hp negative group and had positive correlation between them ( rs=0.739,P<0.01).Conclusion:The relationship between Xinjiang Uygur esophageal squamous cell carcinoma patients with Hp infection and cancer cell malignant degree of proliferation and invasion is close.

Objective: To analyze the effects of the catastrophic disease insurance of New Rural Cooperative Med-ical Scheme ( NRCMS) on alleviating the economic burden of patients in Beijing, and evaluate the effect of the policy implementation. Methods: Based on high, middle and low economic development levels and the implementation pro-ceedings of catastrophic disease insurance of NRCMS, this paper selects 3 districts of Beijing, from which it chooses two or three villages and towns with the highest numbers of catastrophic disease patients, in order to understand the opera-tional status quo of the catastrophic disease insurance through in-depth interviews with managers. This paper conducts questionnaire survey among all catastrophic disease patients from the sampled villages and towns, and uses 497 valid questionnaires received to understand patients' subjective feelings and collect relevant data of family income and medical expenditure of catastrophic disease patients in 2013. Then it carries out data docking with the platform of NRCMS man-agement centers and measure the economic burden of catastrophic disease patients. In this paper, quantitative and qualitative data are analyzed using statistical and thematic analyses, respectively. Results: The catastrophic disease in-surance of NRCMS has significantly played an important role in reducing the average medical expenditure of catastroph-ic disease patients and improving the actual compensation ratio in varying degrees. However, the incidence of cata-strophic health expenditures has not significantly improved. Conclusion: This paper suggests to increase deductibles and reimbursement ratios of catastrophic disease insurance in order to broaden reimbursement directory and other initiatives to improve the compensation level and focus on alleviating the economic burden of catastrophic disease patients.

Objective:To understand the patients'satisfaction on catastrophic medical insurance of New Rural Cooperative Medical Scheme ( NRCMS ) , and provide references for effective management and policy improvement. Methods:According to the principles of representative sampling, a questionnaire survey conducted. 484 patients got compensation for catastrophic insurance in 2013 were surveyed, and information of medical costs was analyzed. Re-sults:The average medical cost accounts for 84. 57% of total household expenditure. Mandatory medical expenditure is still high and economic burden of diseases is heavy. This policy has increased the compensation rate, and the actu-al compensation rate increases 4. 79% in three districts. The average score of patients' attitude toward catastrophic medical insurance is (3.95±1.05) points, 88. 43% of patients continue to participate in medical insurance. Con-clusions and suggestions:Patients' satisfaction on catastrophic medical insurance of NRCMS is high. It is suggested that the existing financing modes should maintain stability in the short term, and the compensatory rate should be im-proved reasonably.

The grass carp (Ctenopharyngodon idella) collectin gene was cloned from mixed liver and kidney cDNA library. The full length sequence of grass carp collectin was 1128 bp, contained a 5' untranslated region of 229 bp and a 3' untranslated region of 104 bp. The open reading frame of grass carp collectin was 795 bp which could code a 264 amino acids polypeptide, including a terminal codon. Phylogenetic analyses showed that grass carp collectin shared the highest homology with that of zebrafish (Danio rerio). To understand the function of grass carp collectin, we expressed and purified the recombinant protein (P(CRD)) that comprised carbohydrate recognition domain (CRD). Agglutination of Aeromonas hydrophila and Staphylococcus aureus etc. and sugars inhibition experiments showed that: galactose, glucose, mannose and maltose could inhibit the agglutination of Aeromonas hydrophila. Maltose could lower the agglutination of Staphylococcus aureus, whereas peptidoglycan and glucose inhibited it well. In addition, the activity of grass carp collectin could not dependent on Ca(2+).
Amino Acid Sequence ; Animals ; Base Sequence ; Carps ; genetics ; Cloning, Molecular ; Collectins ; genetics ; physiology ; Fish Proteins ; genetics ; physiology ; Molecular Sequence Data ; Phylogeny ; Zebrafish ; genetics

Objective To study the cause,prevention and treatment of postpancreaticoduodenectomy hemorrhage.Method The clinical data of 422 patients who underwent PD in our hospital between January 2000 and January 2012 were retrospective analyzed.Results The incidence of postoperative hemorrhage was 8.1％ (34/422),and the mortality was 20.6％ (7/34).Early and delayed hemorrhage occurred in 19 and 15 patients,respectively.Intra-abdominal and gastrointestinal hemorrhage occurred in 20 and 14 patients,respectively.For the 19 patients who underwent reoperation,the mortality was 20.6％ (7/34).When compared with the delayed hemorrhage group,the mortality of the early hemorrhage group was significantly lower (P＜0.05).Conclusions Meticulous operation and reliable hemostasis during operation and prevention of pancreatic fistula,biliary fistula and peritoneal fluid collection after operation are the key points in reducing postoperative hemorrhage.A timely and decisive reoperation is important to manage postoperative hemorrhage.