Objective To detect mite allergens in the dust of air conditioner filter-net and floating air in room. Methods Samples were collected from rooms of asthma patient and normal families with or without air conditioner. Der p 1,Der f 1 and Der 2 were determined by two monoclonal antibody-based ELISA. Results In asthma patient families,the concentration of airborne Der p 1,Der f 1 and Der 2 was (0.23 ? 0.13),(2.62 ? 1.08),(0.93 ? 0.41) ng/m3,and (0.56 ? 0.25),(4.74 ? 1.22),(2.33 ? 0.64) ng/m3 respectively before and after the air conditioner switched on,all showing a significant difference (P