This paper analysed the results of pulmonary aspiration biopsy in 76 cases, whi ch consisted of 23 cases of squamo cell carcinoma, 19 cases of adenocarcinoma, 9 cases of small cell carcinoma, 2 cases of squano-adeno-carcinoma, 4 cases of unclassified carcinoma, 17 cases of benign lesions and 2 cases of normal lung tissue.The accuracy rate in the diagnosis of lung careers with aspiration biopsy in relation to histological typing was emphasized.our results showed that when anticoagulants were added, the samples were double embedding with agar and paraffin, histological section and cytology were studied simultaneously, the accuracy rate was 100% in diagnosis of lung cancer and was 93% in histological typing.

A simple, reliable and sensitive liquid chromatography-isotope dilution mass spectrometry (LC-ID/MS) was developed and validated for quantification of olanzapine in human plasma. Plasma samples (50 microL) were extracted with tert-butyl methyl ether and isotope-labeled internal standard (olanzapine-D3) was used. The chromatographic separation was performed on XBridge Shield RP 18 (100 mm x 2.1 mm, 3.5 microm, Waters). An isocratic program was used at a flow rate of 0.4 m x min(-1) with mobile phase consisting of acetonitrile and ammonium buffer (pH 8). The protonated ions of analytes were detected in positive ionization by multiple reactions monitoring (MRM) mode. The plasma method, with a lower limit of quantification (LLOQ) of 0.1 ng x mL(-1), demonstrated good linearity over a range of 0.1 - 30 ng x mL(-1) of olanzapine. Specificity, linearity, accuracy, precision, recovery, matrix effect and stability were evaluated during method validation. The validated method was successfully applied to analyzing human plasma samples in bioavailability study.