Objective To establish the methods for neonatal screening of glucose 6 phosphate dehydrogenase (G6PD) deficiency. Methods G6PD activity was measured by using fluorescence spot test (FST) with the dry blood sample on the filter paper for neonatal screening. G6PD/6PGD rate test of venous blood samples was further performed for confirmation. Results The positive G6PD deficiency rate was 4.2% and its detection rate was 3.7% in FST for neonatal screening. The conformation rate of FST with G6PD/6PGD rate test for G6PD deficiency was 86.8% and 100% particularly in severe deficiency groups. Both sensitivity and specificity were very high in severe deficiency groups. Conclusions FST is used in neonatal screening of G6PD deficiency because of its high accuracy, applicability, and simplicity Morover, it can test lots of dry blood samples on the filter paper. It is very favorable to diagnose and treat G6PD deficiency early in high incidence districts.

AIM: To establish the quality standard for Shexiangtongbi Babu plaster (Radix Astragali, Radix Angelicae sinensis, moschus, Commiphora myrrha, Resina Boswelliae Carterii, Radix Angelicae Pubescentis, Sanguis Draxonis, etc.) METHODS: Borneolum Syntheticum, Commiphora Myrrha, Radix Angelicae Pubescentis, Sanguis Draxonis, Radix Angelicae Sinensis were identified by TLC, and the content of Muscone was determined by GC. RESULTS: Borneolum Syntheticum, Commiphora Myrrha, Radix Angelicae Pubscentis, Radix Angelicae Sinensis could be identified by TLC. Muscone showed a good linear relationship at range of 0.026?g～0.078?g, r =0.9992. The average recovery was 97.57%, and RSD 1.50% respectively. CONCLUSION: The method is accurate and can be used to control the quality of Shexiangtongbi Babu plaster.

Objective:To investigate the relationship of the changes of peripheral bloos T cell subsets and NK cell with non-Hodgkin's lymphoma(NHL).And to investigate the change of the cell immune function of NHL and chronic lymphadenitis.Methods:Peripheral blood T cell subsets,NK cell with NHL,chronic lymphadenitis patients and 50 normal controls were detected quantitatively with FCM.Results:Compared with the normal controls,the number of CD3~+,CD4~+T cell,CD4~+/CD8~+ ratio markedly reduced,while those of CD8~+T cells increased.These were no significant changes in NK cell.Compared with the group of chrnic lymphadenitis,the number of CD8~+T cell,NK cell markedly increased,while these were no significant changes in CD3~+,CD4~+T cell and reduction of CD4~+/CD8~+ ratio.Conclusion:NHL patients cell function was suppressed.The detection of T cell subsets and NK cells are clinically valuble on the diagnosis,treatment and prognosis of NHL.

Objective To investigate the clinical significance of platelet activation markers CD62p and PAC-1 in patients with hypertension and coronary heart diease.Methods To select patients who were in the Second Affiliated Hospital of Dalian Medical University between January 2012 and September 2012,42 patients with hypertension,46 patients with coronary heart disease.Flow cytometry was used to test the positive percentage of the platelet surface activation markers CD62p and PAC-1 .To observe the difference among hypertension group,coronary heart disease group and health control group;hyper-tension and coronary heart disease group.Results The positive percentage of platelet activation markers CD62p and PAC-1 in hypertension (36.36±9.62,7.18±8.20)%,coronary heart disease group (42.74±14.60,8.81±12.53)% showed sta-tistically significant differences (t=4.150~5.853,P<0.01)compared with healthy control group (26.82±9.13 ,1.09± 1.05)%.The positive percentage of CD62p in coronary heart disease group (42.74±14.60)% was also higher than that of hypertension (36.36±9.62)%,and the difference between them was of statistical significance (t=2.444,P<0.05).Con-clusion The hypertensive patients and coronary heart disease were in pre-thrombotic state,the activation of platelet increase significantly.Molecular markers should be measured in hypertensive patients and coronary heart disease for prevention and treatment of thrombotic disease complication.

无义介导的信使RNA（mRNA）降解途径（nonsense-mediated mRNA decay，简称为NMD)是真核生物细胞内一种重要的基因转录后表达调控机制，它积极参与一系列细胞生理和生化过程，控制细胞命运和生命体的组织稳态。NMD的缺陷会导致人类疾病，如神经发育障碍、肿瘤发生和自身免疫疾病等。UPF3 (Up-frameshift protein 3)是一个核心的NMD因子，它最早在酵母中被发现。UPF3A和UPF3B是UPF3在生物进化到脊椎动物阶段出现的两个旁系同源物，在NMD中具有激活或抑制的作用。以往研究发现，UPF3B蛋白几乎在所有哺乳动物器官中均有表达，而UPF3A蛋白在除睾丸外的大多数哺乳动物组织中难以被检测到。解释这一现象的假说为：在NMD途径中，UPF3B具有比UPF3A更高的竞争性结合UPF2的能力，UPF3B和UPF2的结合促使UPF3A成为游离状态，而游离的UPF3A蛋白不稳定且易被降解。此假说提示UPF3A和UPF3B在NMD中存在拮抗作用。在本研究中，我们重新定量评估了UPF3A和UPF3B在野生型成年雄性和雌性小鼠的9个主要组织和生殖器官中的mRNA和蛋白表达，结果证实UPF3A在雄性生殖细胞中表达量最高。令人惊讶的是，我们发现在包括大脑和胸腺在内的大多数组织中，UPF3A与UPF3B的蛋白水平相当，而在小鼠脾、肺组织中，UPF3A表达高于UPF3B。公共基因表达数据进一步支持了上述发现。因此，我们的研究表明了UPF3A是小鼠组织中普遍表达的NMD因子。同时，该研究结果推测：在生理条件下，UPF3A和UPF3B蛋白之间不存在竞争抑制，且UPF3A在多种哺乳动物组织的稳态中发挥重要作用。
Animals ; Humans ; Mice ; HeLa Cells ; Nonsense Mediated mRNA Decay ; RNA-Binding Proteins/genetics*