Chronic active Epstein-Barr virus infection( CAEBV) is an undefined illness, which is often characterized by severe. chronic, or recurrent infectious mononucleosis-like syndrome, such as fever, hepatosplenomegaly, persistenthepatitis and extensive lymphadenopathy . Patients with CAEBV have high viral loads in their penpheral blood and/ or an unusual pattem of EBV-related antibodies. It is an intractable disease with a poor prognosis. Patients with CAEBV have high mortality from hepatic failure, opportunistic infection, or hemophagocytic lymphohistiocytosis. A definite treatment has not been established . There is accumulating evidence that the clonal expansion of EBV-infected T or natural killer cells play a key role in the pathogenesis of CAEBV.

Mutual information(MI) is a tool of measuring the statistical dependence between two random variables. MI between two images, being considered as two random fields, can be calculated to determine the degree of information redundancy between the image intensities of corresponding pixels in both images. In this paper, we utilize the MI criterion to realize the generalized registration between two neighboring slices in a medical image sequence (CT, MR et al) and consider that they match with each other if the MI between them is maximal. Experimental results confirm performance of our algorithm.

[Objective]To observe the histomorphology and ultrastructure of the intervertebral disc(I D) in the rat model with cervical vertebral unbalance of dynamic and static force.[Method]Sixty SD rates were randomly divided into 4 groups: 3 months,5 months,7 months and the control group.The cervical I D degeneration model was made by destroying the neck muscle of rats and the tissues wer collected every month.The number,area and thickness of the cartilage end-plate were measured by the means of Miyamoto's Classes.The ultrastructure of the apoptosis cells of the intervertebral disc was observed under the electron microscope.[Result]Compared with the control group,the 3 months group showed degeneratin changes,with disordered structure of the annular fibrosis and thickness increase of calcification layer and decrease of blood vessel unmber in the cartilage layer.Complete fibrosis was found in nucleus pulposus in 5 months model group,with the fibro lamellar structure disappeared and few blood vessel buds.The features of 7 months model group was similar to 5 months and osteophyma had been formed near the bordr of part intervertebal.Under the electron microscope,the number of surface projection and organelles was decreased.Fatty drop and apoptotic body could be seen in disc cells of 3 month model group.Few cells,broken collagen fibers in ECM and more cavitation cells for necrosis in 5 months and 7months model groups could be seen.[Conclusion]The cervical I D of model groups has shown typical morphological changes of degeneration and this trend was more serious with the time passing.In the early and middle stage of degeneration,the apoptosis cells can be seen,but in the terminal stage the cellular necrosis was more common.

Since the current worldwide medical education and clinical practice lay a particular stress on training medical students with scientific knowledge and medical technical skills,enormous medical staffs are shaped into medical technical workers who lack medical morality and emotions,leading to the contradiction between medical morality and technologies.Medical personnel should know that medicine not only observes illness closely,but also attaches importance to the patients themselves who are suffering the pain.Medical students should pay attention to the forming process of medical morality and emotion in their preparative stage,accept the exercise to prepare for medical morality,and recalls their love and concern for life.

Objective To identify the role of miR-873, which may regulate the expression of survivin,in human pros-tate cancer PC3 cells invasion.Methods PC3 cells were cultured in vitro, and changes of cellular morphology were ob-served by phase contrast microscope.miR-873,which might regulate the expression of survivin,was predicted by bioinforma-tics and identified using dual luciferase report system.Expressions of miR-873 and survivin were determined using real-time quantitative PCR( qRT-PCR) and Western blotting after transfection of miR-873 mimics.The invasion of PC3 cells was de-tected in vitro by Transwell chamber.Results The expression of survivin was positive by immunofluorescence cytochemis-try.Using dual luciferase reporter system, miR-873 could inhibit the expression of survivin by binding to its mRNA 3′UTR.Results of qRT-PCR and Western blotting showed that overexpression of miR-873 down-regulated the expression of survivin.The invasion of PC3 cells was suppressed by over-expression of miR-873.Conclusion MiR-873 may negatively regulate the expression of survivin in human prostate cancer PC3 cells and inhibit cell invasion.

Objective To identify the role of microRNA-17(miR-17)in human glioma U87 cells invasion which may regulate expression of matriv metalloproteinase(MMP)-2.Methods U87 cells were cultured in vitro,while changes in cellular morphology were observed by phase contrast microscope.The miR-17 which might regulate the expression of MMP-2 was predicted by bioinformatics and identified using dual luciferase report system.Expressions of miR-17 and MMP-2 were determined using real-time PCR and Western blot after transfection of miR-17 mimics.The invasion of U87 cells was detected in vitro by Transwell chamber.Results Expression of MMP-2 was positive by immunofluorescence cytochemistry. Using dual luciferase reporter system,miR-17 could inhibit the expression of MMP-2 by binding to its mRNA 3′UTR. Results of real-time PCR and Western blot showed that over-expression of miR-17 down-regulated expression of MMP-2. The invasion of U87 cells was suppressed by over-expression of miR-17.Conclusion MiR-17 may negatively regulate expression of MMP-2 in human glioma U87 cells and inhibit cell invasion.

The development of medical technology draws the doctor-patient relationship ever closer.However,the abundance and complexity of medical knowledge and technology make it a difficult task for medical personnel to grasp medical knowledge and skills,setting up a higher request for handling physician-patient relationship.Besides,medical disputes between doctors and patients caused by the abuse of medical technology in clinical practice also contribute to the contradiction between the advancement of medical technology and the latency of medical morality.Therefore,it is one important part in the study on medical morality from the perspective of medical technology ethics to take a rational view on the development of medical technology and construct a harmonious physician-patient relationship.

Clinical death of patients often results in such strong psychological stress as anxiety,fear and depression among the family members.Behavior problems incurred by such negative feelings often lead medical disputes.Early psychological intervention upon death to the families can not only protect their mental health but also effectively prevent medical disputes from happening.An analysis of the psychological response and characteristics of such families presents the principles and practice for psychological counseling.Discussions in this regard may inspire hospital administrators in how to prevent medical disputes so incurred.

Objective To investigate the effect of community comprehensive intervention on health behavior and self-rated health status among family caregivers of disabled elderly.Methods One hundredand twenty family caregivers of disabled elderly were selected from community health service centers of Beijing Dongcheng District,and were given 6 month-comprehensive community intervention (intervention group);121 family caregivers of disabled elderly from another community were selected as the control group.The knowledge and skills of caregiving,health behaviors (daily exercise time,daily relaxation time) and self-rated health status were evaluated before and 6 months after intervention.Results The knowledge and skill scores in intervention group were improved by 50.781 points,the daily exercise time and daily relaxation time were prolonged by 0.491 h and 0.837 h,respectively after the intervention (all P＜0.05).There was no significant difference in self-rated health status of two groups before and after intervention (P ＞0.05).Conclusion Community comprehensive intervention improves the knowledge and skills of caregiving,and the health behaviors among family caregivers of disabled elderly,but the intervention measures does not impact the self-rated health status of family caregivers.

Chitosan, a linear polysaccharide, is obtained by chitin N deacetylation. Its ?(1 4) glucosidic bond is not stable and breaks easily in acid condition. In this study, chitosan of different molecular weights(MW) was prepared by acetic acid degradation. Chitosan was dissolved at 3%(w/v) concentration in 1%(v/v) acetic acid and the solution was dried at 50 degree C. We get chitosan membranes of different MW after neutralization with aqueous NaOH. The membranes are transparent, elastic and have good intensity. The permeability studies on chitosan membranes were going on with a permeable install made by our laboratory. It was determined by the transmission concentration of NaCl, L Tyr, glucose and calf albumin. We compared the permeability property with different MW chitosan membranes. The result implied that all of the four samples could permeate through the chitosan membranes. And the permeability property of the membrane increased with the chitosan MW decreaseing.