0.05), but it increased writhing times of mice (P0.05), but HPPT was decreased(P

Objective To investigate changes in the expression of 5-HT2A receptor mRNA and platelet membrane adhesion molecules in migraineurs during the periods of attack and intermittence therapy by Xinzhengtian Pill (XZTP). To study the interrelation between the expression of 5-HT2A receptor mRNA and platelet membrane adhesion molecules. Methods 68 patients were randomly divided into XZTP (therapy) group and Flunarizine (control) group. The RT-PCR method and the image pattern analysis semiquantitation method were used to determine the 5-HT2A receptor mRNA expression levels in the migraineur groups and the normal control group. Flow cytometry was used to detect the expression of whole blood platelet membrane adhesion molecules in those groups. The interrelation between the expression of 5-HT2A receptor mRNA and adhesion molecules was analysed. Results The total effect have show obvious difference between therapy group and control group. 5-HT2A receptor mRNA in the attack group was higher than that of the intermittence group and the normal group, and XZTP can reduce this expression. The expression of whole blood platelet membrane adhesion molecules had a higher increase during the periods of attack and intermittence, compared with the normal control group and XZTP. The 5-HT2A receptor mRNA expression level in the migraineur groups had a high positive correlation with CD41 and moderate positive correlation with CD62P. Conclusion 5-HT2A receptor mRNA and platelet membrane adhesion molecule expression level during the period of attack was significantly augmented and platelet activation was increased. XZTP maybe restrained the expression of above indexes and resulted in platelet activation reduced.

OBJECTIVETo explore the clinical diagnostic features and treatment of desmoplastic small round cell tumor (DSRCT), and to improve the understanding and management of this tumor.

METHODSThe clinicopathological data of nine patients treated in our hospital from October 2004 to June 2014 were retrospectively analyzed and a review of the literature was made. The clinical manifestations, pathological characteristics, diagnosis and differential diagnosis, treatment and prognosis of this tumor were summarized and analyzed.

RESULTSNine patients with DSRCT, 5 males and 4 females, with an average age of 21 years (range 8-56 years) were included in this study. Ultrasound examination revealed irregular low-density mass shadow in the abdominal cavity. CT examination found that 6 cases had abdominal and retroperitoneal multiple solid tumor nodules, uneven density, and visible low density fluid area. Postoperative pathological examination revealed that the tumor cells were small, mostly elliptic, gathered to form clear structure of nests with clear irregular boundaries. The central portion of large tumor nests often showed necrosis. Scattered fibroblasts and large amount of hyalinization of collagen fibers were seen in the interstitial tissue around the nests. Six patients received laparotomy surgery, however, all failed to resect the tumor completely. Three patients received postoperative chemotherapy, i. e. two cases had carboplatin and paclitaxel chemotherapy, and one case of chemotherapy regimen not specified. Two patients had radiation and chemotherapy (no concrete plan was available). Another case was lost to follow-up. Two of the three patients without surgery received chemotherapy with CAP (cyclophosphamide+adriamycin+carboplatin) and total rectal lesions, pelvic and inguinal lymph nodes, ilium metastases radiation therapy. Another one patient received EP regimen (DDP+VP16) which was then changed into a TP chemotherapy alone. Eight of the nine cases died shortly after surgery, and only one patient treated with chemotherapy alone was still alive after 11 months of follow-up.

CONCLUSIONSDesmoplastic small round cell tumor is a very rare, special type of soft tissue tumor, with very poor prognosis. This tumor may be preliminarily diagnosed according to the imaging characteristics and detection of tumor markers, however, final diagnosis is made by pathology. Surgery is the priority of treatment, combined with complementary radiation and chemotherapy.

Abdominal Neoplasms ; complications ; diagnosis ; mortality ; therapy ; Adolescent ; Adult ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Biomarkers, Tumor ; analysis ; Carboplatin ; administration & dosage ; Child ; Combined Modality Therapy ; methods ; Cyclophosphamide ; administration & dosage ; Desmoplastic Small Round Cell Tumor ; complications ; diagnosis ; mortality ; therapy ; Doxorubicin ; administration & dosage ; Female ; Humans ; Male ; Middle Aged ; Paclitaxel ; administration & dosage ; analysis ; Prognosis ; Retrospective Studies