Objective To observe the efficacy of Botulinum toxin type A(BTXA)injection in Meige's syndrome and facial wrinkles and exp lore the method of reducing the side e ffect of BTXAand weaken facial wrink les.Methods Ninety-nine patients were evaluate d on the therapeutic efficacy of single and multiple topical facial in-jection with BTXA.Results The effective rates of patients with blepharospasm were 98.4%,oromandi bular dystonia 90.9%,blepharospasm and o romandibular dystonia 95.8%,respectively.The therapeutic effect for each disease lasted(16.9?8.4)weeks?(12.6?5.4)weeks and(13.5?5.7)weeks,respectively.Facial wrinkl es of all patients has got weakened and seemed much smooth and flattend.The rec overy rate approached to 100%.The local side effects were transient an d mild,and disappeared spontaneously.Conclusions The treatment of Meige's syndrome and facial wrinkles with BTXA could produce a significant impro vement,and is safe.Strictly observ e the procedures of injection,and use adequate dose of BTXA,the side effects o f BTXA can be decreased.

Objectives To investigate the relationship between different exercise types and the attention network.Methods A total of 75 university students were enrolled and randomly divided into an open-skill exercise group,a closed-skill exercise group and an irregular exercise control group,each of 25.They underwent the exercise as their group names implied.Then the attention network test was performed to evaluate their functions of the alerting network,orienting network and executive control network.Results Significant differences were found in the function of the executive control network among the 3 groups,while no significant difference was found among the 3 groups in the functions of the alerting network and orienting network.Conclusions Both open-and closed-skill exercise are associated with better function of executive control network.Furthermore,open-skill exercise showes more benefits than closed-skill exercise in promoting the function of the executive control network.Neither opennor closed-skill exercise bring benefits to the function of the alerting or orienting network.

Objective To summarize X-ray,CT finding of mediatinal emphysema in the neonate.Methods The X-ray,CT finding of 16 cases with mediatinal emphysema in the neonate were anolysed retrospectively.Results X-ray finding of 16 cases were:around diaphram with a low density band 13 cases,with spinnaker sign 8 cases,with continous diaphram sign 3 cases,there were only little emphysema in anterior-mediatinal 3 cases,accompany with pneumothorax 5 cases.CT imaging of 3 cases was a low density image in diaphram that was CT value was negative(-500～-900Hu).Conclusion Radiography of chest is the first method about diognosis mediatinal emphysema in the neonate,profile chest is more profit to diagnose little emphysema in anterior-mediatinal,CT can diagnose further clear.

Objective To evaluate the efficacy of different duration of low-carbohydrate diet (LCD) intervention on glycosylated hemoglobin (HbA1c) in type 2 diabetes mellitus (T2DM) patients. Methods Randomized controlled trials (RCTs) of LCD intervention in T2DM patients were collected in the databases such as MEDLINE, PubMed, OVID, China National Knowledge Infrastructure, China Scientific Journal Database by VIP, Wanfang database, et al.Data were analyzed by RevMan 5.3 version. Results Eight RCTs were included. The results of Meta-analysis indicated that the effects of lowering HbA1c by LCD intervention for three (Z=2.28, P<0.05) and six months (Z=14.99, P<0.01) were better than other diabetes diets, but there was no significant statistical difference between one (Z=0.65, P=0.51) and two years (Z=1.62, P=0.10). Conclusions Hypoglycemic effect of short-term LCD was better than other diabetes diets, but long-term effect was similar between them. LCD was a therapeutic diet suitable for T2DM patients.

The widespread availability of multi-detector spiral CT and its post processing techniques,and MRI,provide us with important tools to carry out anatomical studies on pancreaticobiliary maljunction (PBM).Determining the relationship between PBM and biliary diseases contributes not only to a better understanding of pathogenesis and pathological process of biliary diseases,but it also provides evidence for prophylactic treatment.The present study reviewed the definition,classification,pathological anatomy of PBM,relationship between PBM and pancreaticobiliary diseases,and clinical treatments of PBM.

In recent years,exciting research progress has been made on hepatic Kupffer cells.For inflammation,the study of Kupffer cells focused on its mechanism of whether and how Kupffer cells play a role in regulating inflammatory reaction,especially molecular mechanisms.For fatty liver,researchers are exploring the role of Kupffer cells in the development of fatty liver,as well as potential treatments.For the treatment of ischemia-reperfusion injury,latest protocols were based on Kupffer cells.In the area of liver transplantation,the study of Kupffer cells centered on immune tolerance and graft versus host disease (GVHD).As for tumor,current studies focused on regulatory mechanism of Kupffer cells on growth and metastasis of tumor.This article will present a review on the latest progress.

Objective To study the effect of UHRF1 expression inhibition by RNA interference on the radiosensitivity of esophageal cancer cell line TE-1 and its mechanism.Methods Short hairpin RNA (shRNA) targeting UHRF1 gene was introduced into TE-1 cells by lentivector-mediated transfer.The cells were divided into three groups:non-transfected group,negative control (NC)-shRNA-transfected group,and UHRF1-shRNA-transfected group.The mRNA and protein expression levels of UHRF1 in TE-1 cells were measured by RT-PCR and Western blot before and after transfection.After transfection and X-ray radiation,the radiosensitivity of TE-1 cells was evaluated by colony formation assay; the cell cycle and cell apoptosis were determined by flow cytometry; the γ-H2AX (as a marker of DNA damage) level was measured by Western blot.Results After transfection with UHRF1-shRNA,the mRNA and protein expression levels of UHRF1 were significantly decreased in TE-1 cells,as compared with those in the NC-shRNA-transfected group and non-transfected group (0.11 vs 0.96 and 0.98,F =124.21,P =0.000;0.10 vs 0.89 and 0.94,F =125.25,P =0.000).The UHRF1-shRNA-transfected group had sensitization enhancement ratios of 1.53 (D0 ratio) and 1.95 (Dq ratio).X-ray radiation could cause G2/M arrest and increase apoptotic rate and γ-H2AX expression in TE-1 cells.Compared with the two control groups,the UHRF1-shRNA-transfected group showed significantly less G2/M arrest (F =500.15,P =0.000),a significantly higher apoptotic rate (F =100.10,P =0.000),and significantly higher residual γ-H2AX expression (F =61.00,P =0.000) at 24 hours after X-ray radiation.Conclusions RNA interference can effectively inhibit the UHRF1 expression and enhance the radiosensitivity of TE-1 cells.The mechanism may be related to cell cycle regulation,cell apoptosis,and DNA damage repair.

Objective To study and evaluate the ability of allogeneic bone marrow stromal cells(BMSCs) to survive and regenerate bone in muscles without using immunosuppressive agents. Methods A complete mismatch between donor BMSCs and recipient rabbit was confirmed by one way mixed lymphocyte reaction assaysprior to implantation. And then bone marrow aspirates were obtained from donor rabbits.BMSCs were isolated from the bone marrow, cultured in conditional medium to be ubdyced to become osteogenic, and then to examine these characteristics. After that the donor BMSCs were transplanted into the recipient rabbits. Immunological tests such as lymphocyte transformation rate and cell mediated cytotoxity, histological observation, seeding cells survival and bone formation were performed following transplantation. Results Allogeneic BMSCs transplantation did not actually elicit an adverse immune response, and bone regenerated at the transplantation area, and then the transplanted BMSCs marked with BRDU preoperatively were founded to be living 8 weeks later after transplantation using immunohistochemistric technique. Conclusion Allogeneic BMSCs would not elicit an adverse immune response in vivo without the immunosuppressive therapy, which could survive and form new bone tissue with the help of BMP-2 in the muscles.

objective Using iodimetric analysis of dual-energy CT,to explore the dual perfusion amount and proportion of hepatic artery and portal vein in different hepatic lobes on normal living bodies.Methods A total of 77 patients without hepatic diseases underwent contrast-enhanced upper abdomen dual-energy CT scanning.The raw data were transferred to the workstation for postprocessing.ROI were selected,then the iodine content in arterial phase,portal phase and delay phase were calculated automatically.The differences of these measures (iodine content and hepatic artery to portal vein perfusion ratio of the left hepatic lobe,right hepatic lobe and caudate lobe) in the left,fight and caudate lobe of liver were detected by using ANOVA test.Results The iodine concentration in the caudate lobe was(851 ± 35)μg/L from hepatic artery and (2912 ± 78) μg/L from portal vein.The iodine concentration in the left hepatic lobe was (445 ± 34) μg/L from hepatic artery and (2373 ± 77) μg/L from portal vein.The iodine concentration in the right hepatic lobe was(504 ± 30)μg/L from hepatic artery and(2515 ± 78) μg/L from portal vein.The perfusion condition (amount of blood supply) of caudate lobe showed a significant statistic difference from the left and right hepatic lobe (P ＜ 0.05),and the amount of blood supply from both sources were more than those of the left and fight hepatic lobes.There was no significant statistic difference in the amount of hepatic artery and portal vein blood supply between the right and left hepatic lobe(P ＞ 0.05).The proportions of blood supply from hepatic artery and portal vein (hepatic artery/portal vein) were different among the three hepatic lobes,which was (28.41 ± 3.42) ％ in left lobe,(35.76-± 5.80) ％ in fight lobe and (49.92 ±4.63)％ in caudate lobe,respectively(F =5.36,P ＜0.01).Conclusion Dual-energy CT can be used to study the dual-perfusion condition of the liver.On normal living bodies,the hepatic artery and portal vein perfusion in caudate lobe are different from those in left and right lobes.

Objective Hepatocellular carcinoma with bile duct tumor thrombus (BDTT) is rare,and surgical treatment is currently considered as the most effective treatment.Whether resectional surgery should be carried out on these patients remains controversial.Therefore,this Meta-analysis aimed to find out the long-term survival after resectional surgical treatment.Methods We conducted a literature search on PubMed,Embase and Web of Science from inception to September 2016.11 studies were included which involved 5295 patients.Each study was evaluated using the Newcastle-Ottawa Scale.The pooled effect was calculated and the associations between BDTT and overall survival (OS) or disease-free survival (DFS)were reevaluated using Meta-analysis with hazard ratio (HR) and 95％ confidence interval (CI).Results The HR for OS and DFS was 2.34 and 1.81,the 95％ CI were 1.26 ～ 4.36 and 1.17 ～ 2.78,respectively.Conclusion HCC patients with BDTT had a bad prognosis after hepatic resection or liver transplantation.