Objective To study the effects of density,phosphorous(P),and nitrogen(N)fertilizers on yield and quality of Andrographis paniculata.Methods Randomized block design in yield was used and HPLC was used to analyze the content of andrographolide and dehydroandrographolide.Results Plant height,fresh weight,dry weight,content of dehydroandrographolide were influenced significantly by density.The quality character and economic yield of content of andrographolide,dehydroandrographolide,total content of both andrographolide and dehydroandrographolide were significantly influenced by P nutrition.The economic yield of dry weight,content of andrographolide and dehydroandrographolide,and total content of both andrographolide and dehydroandrographolide were influenced significantly or extreme significantly by N nutrition.P and the interaction of P and N fertilizer extremely influenced the economic yield.The relative influences between plant weight and dry weight was extremely significance,that between quality character and quality character,that between quality character and economic yield were significant or extremely significant.Conclusion When planted with 1.8?105 /hm2,fertilizered with P2O5 540 kg/hm2 and pure N 225 kg/hm2,the hightest economic yield is gained.

Objective To study the change of liver micro-circulation in liver cancer patients after interventional therapy by using CT perfusion imaging.MethodThe patients were dividecl into two groups: therapy group and control group.Each group had 30 patients.CT perfusion imaging scanning was done for the patients by using the Light speed 16 CT with the MSCT "Toggling-table" technology.The data was analyzed by single variance.The interested area of liver tissue was chosen as area without iodine after interventional therapy.The parameters of perfusion imaging were MTT,BF,BV,PS and HAF.ResultsSignificance of BF and BV existed in the liver tissue between therapy group and control group.The relatively normal liver tissue and the tissue in the same side had significance of BF,BV,MTT,PS in the two groups.In the therapy group,significance of BF,BV existed in the liver tissue of both side.ConclusionThe interventional therapy combined with Chinese traditional medicine can raise life quality of the patients,relieve the clinical symptom and improve the physical sign.The perfusion imaging of liver cancer,as a simple,rapid,no trauma methed,can supply the blood perfusion information of various positions as well as displaying anatomic details,which has provided a new kind of method for evaluating the effect of chemoembolization and traditional Chinese medicine.

AIM:To investigate the changes of connexin 43 (Cx43) via establishing a model of sympathomi-metic atrial fibrillation ( AF) .METHODS:The mongrels ( n=15) were randomly divided into control group , rapid atrial pacing (RAP) group and isoprenaline (ISO) perfusion+RAP group (ISO+RAP group).All mongrels’ hearts were taken out rapidly by median sternotomy to establish the cardiac model with Langendorff perfusion in vitro.The atrial effective re-fractory period ( AERP) and AF inducability were tested .The expression and distribution of tyrosine hydroxylase ( TH) were analyzed by immunohistochemistry .Total protein level of Cx 43 and phosphorylation of Cx 43 were determined by West-ern blot.The distribution of Cx43 were also observed by immunofluorescence staining .The cell apoptosis was analyzed by TUNEL staining.The generation of reactive oxygen species ( ROS) in the mitochondria was measured by fluorescence spec-trophotometry .RESULTS:No significant change of AERP was found between control group and RAP group , while that in ISO+RAP group was significantly decreased (P<0.05) and induced AF.Compared with control group, the expression of TH, apoptotic index and the generation of ROS increased gradually (P<0.05), while the content of Cx43 decreased grad-ually both in the total protein and the phosphorylation levels in RAP group and ISO +RAP group (P<0.05).The fluores-cence intensity of Cx43 was also attenuated and Cx43 were lateralized apparently in RAP group , while Cx43 were character-ized as punctate distribution in ISO +RAP group.CONCLUSION:Sympathetic nerves may activate autophagosome at in-tercalated discs and trigger cell apoptosis , resulting in remodeling and downregulation of Cx 43 via oxidative stress , thus having effects on mediating and maintaining AF .

Objective To investigate the role of fibroblast growth factor-23 (FGF23) in secondary hyperparathyroidism (SHPT). Methods (1)Serum FGF23 and intact parathyroid hormone (iPTH)from 38 maintenance hemodialysis (MHD) patients were measured by ELISA and chemiluminescence enzyme immunoassay respectively.(2) Parathyroid cells from six SHPT patients underwent parathyroidectomy with forearm autotrlansplantation were cultured for 24 h,then were induced by 0.1 mg/L FGF23.The supernatant was collected at 0.6,12,24 and 48 h respectively. The concentration of iPTH was measured by chemiluminescence enzyme immunoassay. (3)Protein expression of Klotho,FGFR1,FGFR3,GATA-3 and PCNA in parathyroid tissue from 33 SHPT eases and 3 healthy people were detected by immunohistochemistry SP and PV methods respectively. Positive cell rate and absorbance were calculated. Results (1) Serum FGF23 [(3901.85±2618.11) ng/L] was positively correlated with serum iPTH [(460.00±489.77) ng/L] in MHD patients. (2) 0.1 mg/L FGF23 suppressed iPTH secretion of parathyroid cells only at 24 h time point in vitro (P<0.05). (3) Expression of GATA-3, FGFR3, Klotho and PCNA was significantly increased and FGFRl was signiticantly decreased in parathyroid tissue of SHPT-patients as compared to healthy people. (4) Positive cell rate of GATA-3 was positively correlated with iPTH (r~2=0.1901, P=0.0425) and PCNA (r~2=0.2584, P=0.0025). Klotho was positively correlated with FGFRI and FGFR3 (r~2=0.2046, P=0.0082;r~2=0.2833, P=0.0014). PCNA was negatively correlated with FGFR1 (r~2=0.1292, P=0.0399) and positively correlated with FGFR3 (r~2=0.1226, P=0.0457). FGFR1 was negatively correlated with serum phosphate (r~2=0.2329, P=0.0044) and positively correlated with serum calcium (r~2=0.1422, P=0.0305). Conclusions FGF23 level is positively correlated with iPTH level in MHD patients. FGF23 can inhibit iPTH secretion of parathyroid cells in a weak and short way, which may be associated with the proliferation of GATA-3 positive cells and parathyroid cells, the up-regulation of FGFR3 and the down-regulation of FGFR1 expression.

The power and signal transmission technology is one of the key technologies in neuroprosthetic research. This paper proposes firstly the related theory of power and signal transmission technology in neuroprosthetic, then summarizes the three key aspects of the power and signal transmission technology in neuroprosthetic. After analyzed the development of the inductive wireless power harvesting technology, the wireless telemetry technology and the wireless power harvesting telemetry technology, the emphasis on research contents will be proposed and discussed, which will help accelerate the further research of prosthetic.
Electric Power Supplies ; Electrodes, Implanted ; Humans ; Neural Prostheses ; Prosthesis Design ; instrumentation ; Signal Processing, Computer-Assisted ; instrumentation ; Telemetry ; instrumentation ; Wireless Technology ; instrumentation

Objective:To summarize the patient profiles and therapeutic efficacies of ABO-incompatible living-related kidney transplantations at 19 domestic transplant centers and provide rationales for clinical application of ABOi-KT.Methods:Clinical cases of ABO-incompatible/compatible kidney transplantation (ABOi-KT/ABOc-KT) from December 2006 to December 2009 were collected. Then, statistical analyses were conducted from the aspects of tissue matching, perioperative managements, complications and survival rates of renal allograft or recipients.Results:Clinical data of 342 ABOi-KT and 779 ABOc-KT indicated that (1) no inter-group differences existed in age, body mass index (BMI), donor-recipient relationship or waiting time of pre-operative dialysis; (2) ABO blood type: blood type O recipients had the longest waiting list and transplantations from blood type A to blood type O accounted for the largest proportion; (3) HLA matching: no statistical significance existed in mismatch rate or positive rate of PRA I/II between two types of surgery; (4) CD20 should be properly used on the basis of different phrases; (5) hemorrhage was a common complication during an early postoperative period and microthrombosis appeared later; (6) no difference existed in postoperative incidence of complications or survival rate of renal allograft and recipients at 1/3/5/10 years between ABOi-KT and ABOc-KT. The acute rejection rate and serum creatinine levels of ABOi-KT recipients were comparable to those of ABOc-KT recipients within 1 year.Conclusions:ABOi-KT is both safe and effective so that it may be applied at all transplant centers as needed.