Objective To investigate the prevention of re-adhesion after high dose estradiol valerate and hysteroscopy regular inspection after separation of severe intrauterine adhesions.Methods A total of 97 severe intrauterine adhesions patients after transcervical resection of adhesions were divided into group A,B,C:group A placed intrauterine device and oral intook progynova 1 mg/d after 2 days of surgery,3 times/d,for 3 months; group B placed intrauterine device and oral intook progynova 3 mg/d after 2 days of surgery,3 times/d,for 3 months; group C with the same treatment as group B with further hystemscopy regular inspection every month.Regular follow-up data were collected in the following 3 months to compare endometrial thickness,re-adhesion occurrence and treatment effect in different groups.Results After 3 months of treatment,endometrial thickness of group B and group C was higher than that in group A [(0.83 ± 0.23),(0.99 ± 0.28) cm vs.(0.54 ± 0.18) cm],group C was higher than group B,and there were significant differences (P＜ 0.05).The effective rate in group B and group C was higher than that in group A [75.00％(24/32),90.32％(28/31) vs.61.76％(21/34)],group C was higher than group B,and there were significant differences (P＜ 0.05).The occurrence rate of re-adhesion in group B and group C was lower than that in group A [21.88％(7/32),9.68％(3/31)vs.38.23％(13/34)],group C was higher than group B,and there were significant differences (P ＜ 0.05).Conclusion The prevention of re-adhesion after high dose estradiol valerate and hysteroscopy regular inspection is effective and superior.

There is a complicated association between the liver and diabetes mellitus.Diabetes mellitus may cause various forms of liver damage,such as nonalcoholic fatty liver disease (NAFLD),hepatic cirrhosis,hepatocellular carcinoma,liver abscess,and liver transplant complications.With the growing epidemic of diabetes mellitus,this review investigates diabetes mellitus induced liver damage.

BACKGROUND:Bone formation and development are reported to be regulated by bone morphogenetic protein2(BMP2)-induced Osterix expression. OBJECTIVE:To investigate the regulatory effect of BMP2/Osterix signaling pathway on differentiation of preosteoblasts in mice. METHODS:mRNA and protein expression of Osterix wasdetermined by real-time RT-PCR and western blot assay, respectively at various time points after mouse preosteoblasts were treated with BMP2. pcDNA3.1/myc-Osterix eukaryotic expression vector was constructed and transducted into preosteoblasts, and then mRNA expression of alkaline phosphatase, bone sialoprotein, and matrix extracelular phosphoglycoprotein wasdetected by real-time RT-PCR after transduction and BMP2 treatment. RESULTSANDCONCLUSION:Osterix mRNA expression was up-regulated when treated with BMP2 in mouse preosteoblasts, and reached the peak at 24 hours. In addition, the protein expression of Osterix was increased after BMP2 treatment. Alkaline phosphatase, bone sialoprotein, and matrix extracelular phosphoglycoprotein mRNA expression wasup-regulated after transfection of mouse preosteoblasts with pcDNA3.1/myc-Osterix eukaryotic expression vector and BMP2 treatment. Our results indicate that BMP2 regulates the synthesis of genetic markers of osteogenesis,such asalkaline phosphatase,matrix extracelular phosphoglycoproteinviaup-regulating Osterix expression in mouse preosteoblasts, suggesting BMP2/Osterix signaling pathway plays a critical role in bone development.

Objective To study the length of hypertension history and its related factors in patients with both ischemic cerebral vascular disease(ICVD) and hypertension.Methods The profile of hypertension history in ICVD patients from our hospital between 1990 and 1999 was summarized.Hypertension history meant the time interval between onset of first symptomatic ICVD and establishment of diagnosis of hypertension.The related factors' influence on the length of hypertension history was analyzed by means of multiple linear regression model.Results In ICVD patients with hypertension,the mean length of hypertension history was 14.29?10.63 years,the median was 11 years.In multiple linear regression model,increased age when hypertension was diagnosed,diabetes mellitus,smoking,peasant(army man as control group) were independent factors that shortened hypertension history.Antihypertensive treatment independently lengthened hypertension history.Conclusion The length of hypertension history varies greatly,50% of patients developed the first ICVD in 11 years after the diagnosis of hypertension was established .Coexisting smoking and diabetes mellitus hasten while control of arterial blood pressure postpone onset of ICVD in patients with hypertension.

Objective To study the pattern of cellular proliferation and apoptosis in different part of colorectal cancer and its relation to patients' prognosis. Methods The expression of PCNA and Fas protein were investigated by immunohistochemistry in cancer mucosa, cancer adjacent mucosa and cancer distant mucosa of 76 cases of colorectal cancer, and the patients prognosis were investigated too. Results The PCNA expression in cancer mucosa is significantly higher than that in cancer adjacent tissue(P

objective Using iodimetric analysis of dual-energy CT,to explore the dual perfusion amount and proportion of hepatic artery and portal vein in different hepatic lobes on normal living bodies.Methods A total of 77 patients without hepatic diseases underwent contrast-enhanced upper abdomen dual-energy CT scanning.The raw data were transferred to the workstation for postprocessing.ROI were selected,then the iodine content in arterial phase,portal phase and delay phase were calculated automatically.The differences of these measures (iodine content and hepatic artery to portal vein perfusion ratio of the left hepatic lobe,right hepatic lobe and caudate lobe) in the left,fight and caudate lobe of liver were detected by using ANOVA test.Results The iodine concentration in the caudate lobe was(851 ± 35)μg/L from hepatic artery and (2912 ± 78) μg/L from portal vein.The iodine concentration in the left hepatic lobe was (445 ± 34) μg/L from hepatic artery and (2373 ± 77) μg/L from portal vein.The iodine concentration in the right hepatic lobe was(504 ± 30)μg/L from hepatic artery and(2515 ± 78) μg/L from portal vein.The perfusion condition (amount of blood supply) of caudate lobe showed a significant statistic difference from the left and right hepatic lobe (P ＜ 0.05),and the amount of blood supply from both sources were more than those of the left and fight hepatic lobes.There was no significant statistic difference in the amount of hepatic artery and portal vein blood supply between the right and left hepatic lobe(P ＞ 0.05).The proportions of blood supply from hepatic artery and portal vein (hepatic artery/portal vein) were different among the three hepatic lobes,which was (28.41 ± 3.42) ％ in left lobe,(35.76-± 5.80) ％ in fight lobe and (49.92 ±4.63)％ in caudate lobe,respectively(F =5.36,P ＜0.01).Conclusion Dual-energy CT can be used to study the dual-perfusion condition of the liver.On normal living bodies,the hepatic artery and portal vein perfusion in caudate lobe are different from those in left and right lobes.

0.05) respectively.Conclusion:Distraction osteogenesis can be used for alveolar ridge augment and for the application of dental implant.

The original methods on DNA extraction are not only complex and expens ive, but although invalid with actinomycetes under extreme environments Genomi c DNA can be extracted directly from biomass picked up from solid medium by micro wave thermal shock The extracted DNA is suitable for PCR The method is effec ti ve, easy and fast, and can be used in actinomycete systematics and identificatio n rapidly

Objective To investigate the animal model of acute lung injury (ALI) induced by caudal vein injection of trypsin in rats and to evaluate the model.Methods The model of lung injury was established by caudal vein injection of trypsin in rats.The rats were killed at the time point of 3 h,6 h,12 h,24 h,and 24 h and then the pathological changes of structure of lungs,peripheral blood neutrophil count,arterial blood gas analysis and lung wet/dry (W/D) weight ratio in rats were measured and observed.Results The results of hematoxylin eosin (HE) staining showed that there was no obvious pathological changes in lung tissues of the control group,while alveolar and pulmonary septal edema,thickening,and a large number of inflammatory cells infiltration in the lung tissues of the model group.Compared to the control group,the peripheral blood neutrophil counts,W/D and PaCO2 were significantly increased,PaO2 was significantly decreased (P ＜0.01).There was significant differences in the number of peripheral blood neutrophils,PaCO2,W/D and PaO2 between the model groups (P ＜ 0.01).Conclusions The rat model of ALI induced by trypsin can successfully simulate the lung damage caused by the release of a large number of trypsin when severe acute pancreatitis occurred.