Objective:To study the application of Gomori special staining in the late bell stage of dental germ,tooth and periodontium.Methods:Paraffin-embedded sections of human dental germ in late bell stage,tooth and periodontium of mature rats were stained by improved Gomori and Mallory special staining method.Results were observed by light microscope.Results:Improved Gomori special staining method can easily discriminate enamel matrix and dentin matrix in late bell stage of dental germ,sclerous tissues and soft tissues in mature tooth and periodontium,bone and cartilage in two different colouration.Cells and tissues can be observed clearly.Conclusion:Dental germ,tooth and periodontium can be stained by improved Gomori special staining with satisfactory results.