Four hybridomas which produce the monoclonal antibodies against C-reactive protein(CRP)were reported.ELISA was used to screen the hybridomas secreting monoclonal antibodies to CRP,and subcloning was carried out by limited dilution. These two sets of monoclonal antibodies by GHB7 and GHB10 were purified from ascites by the use of QAE-Sephadex A50.The purity of these two sets of antibodies which was tested by SDS gel elctrophoresis,showed one band only.Isoelectrefocus- ing was performed and showed that IP of the two types of antibodies is about pH 5.0-5.5,The subclass of four antibodies was IgG2b,IgG2a IgA,IgM respectively. The experimental conditions of preparation of hybridomas were discussed.

Objective: Interleukin-10 (IL-10) is an anti-inflammatory helper T cell type 2 (Th2) cytokine that modulates Th1-type cytokine production. The purpose of present study was to detect the expression and changes of IL-10 in the rats heart transplantation, as determine the role of IL-10 in the acute rejection of rats heart transplantation. Methods: Hearts from DA rats were transplanted into the necks of Wistar recipients. Allografts was divided into five groups: group A (control, n=12), group B (DST treated, n=12), group C (IL-2 Mab treated, n=12), group D (IL-4 Mab treated, n=12), group E (IL-2 Mab treated, n=12). The local expression of IL-10 was analyzed at day 1,3,5,7,9,11,14 by reversed transcripition polymerase chain reaction (RT-PCR). Results: Allografts survival time was (8.3±1.7) days, (29.2±7.1) days, (26.4±5.7) days, (10.2±1.9) days and (55.0±10.6) days (P＜0.05， P＜0.01), respectively. When compared with group A, the level of IL-10 was upregulated in group B, group C and group E, especially in group E. The upregulation of IL-10 was related to the grafts survival time. Conclusion: IL-10 plays an important role in the development of acute transplantational rejection, suggests that Th-2 type deviation may contribute to the prolongation of graft survival.

Objective To review the clinical and laboratory characteristics of the hemohemocytopenia diseases in children ,in or-der to improve the understanding of the related diseases and the level of diagnosis and treatment .Methods A total of 217 children with hemocytopenia diseases with one ,two or three series of cell reducing ,and the degree of blood cells reduce with anemia and common diseases in children .Results In 185 cases with the hemocytopenia diseases ,1 series reduce were mainly in idiopathic thrombocytopenic purpura(ITP ,37 .2% )、thrombocytopenia (19 .8% ) ,granulocytopenia or agranulocytosis (9 .1% ) .2 series re-duces mainly in acute leukemia (43 .6% ) ,the IT P (21 .7% ) and aplastic anemia (6 .5% ) .3 series reduce mainly in acute leukemia (38 .9% ) and aplastic anemia(33 .6% ) .Common cause of anemia in children with 180 cases of hemohemocytopenia diseases were a-cute leukemia (20 .0% ) ,the ITP(19 .4% ) and no clinical diagnosis of anemia (16 .1% ) .Conclusion After analyzing the character-istics of the patient′s clinical and laboratory in 217 cases of hemocytopenia diseases ,improve the clinical blood disease related knowl-edge ,help understand the laboratory data and proportion .

Objective To appraise the values of combined determination of cTnI,MYO and CK-MB mass in the diagnosis of viral myocarditis.Methods The myocardial injury markers(cTnI,MYO and CK-MB mass)and myocardial enzyme(LDH,AST,CK and ?-HBDH)of 61 patients with viral myocarditis(VMC),74 patients with non-viral myocarditis(NVMC)and 46 healthy subjects were determined,respectively,by microparticle chemiluminescent immunoassay and continuous monitoring assay.Diagnostic efficiency was calculated by matrix decision method.Results The serum levels of cTnI,MYO and CK-MB mass of the patients in VMC group were obviously higher than that of the patients in NVMC group(0.46?0.21 vs 0.06?0.04?g/L,98.7?38.2 vs 39.2?26.8?g/L and 6.1?4.2 vs 2.2?1.7?g/L,respectively)as well as that of healthy subjects(0.46?0.21 vs 0.07?0.04?g/L,98.7?38.2 vs 36.5?24.7?g/L and 6.1?4.2 vs 2.1?1.5?g/L,respectively,P0.05).For preliminary diagnosis of VMC,the SE of MYO is highest,but the SP of cTnI and CK-MB mass were better choice.Restoration of MYO was earliest while cTnI was latest during convalescence of the patients.Conclusion Combined and uninterrupted determination of the myocardial injury markers can provide better diagnostic accuracy.

Objective To evaluate the performance verification of Mindary BC5390 hematology analyzer .Methods According to requirement of CLSI documents ,the precision ,accuracy ,linearity ,carryover were evaluated .Results The background counts all met the designed requirements of manufacturer .Precision ,accuracy ,linearity of the WBC ,RBC ,PLT ,Hb ,HCT ,MCV were good and the contamination carrying rates were low .Except for basophils ,the classification of leukocyte was well correlated with the artificial microscopy classification(r2 >0 .95) .Conclusion The BC5390 is a ideal automated hematology analyzer which has high precision and accuracy ,wide linearity ,low contamination carrying rate ,and it can be used for the blood samples analysis .

Objective Through analyzing and summarizing the main pathogens of bacterial infections of children in Sichuan Province and trends of drug resistance in 2013 to provide a reference for the clinical use of antibiotics.Methods The pediatric pathogen were collected by member of Sichuan province in china antimicrobial resistance surveillance system.Results A total of 22 470 clinical bacterial were isolated from the members,in which Staphylococci,Escherichia coli,Streptococcus pneumoniae,Kleb-siella pneumoniae,Hemophilus influenza,Moraxella catarrhalis were the most common Bacteria.The resistance rates of Staphylo-coccus aureus and Coagulase-negative staphylococci to oxacillin were 1 7.7% and 71.1%.4 strains of Staphylococcus aureus and 20 strains of coagulase-negative staphylococci were teicoplanin resistance.Escherichia coli and Klebsiella pneumoniae were clearly re-sistant to the third generation cephalosporins,ampicillin and ampicillin/sulbactam,with the exception of ceftazidime.Carbapenems remained highly active against all the target bacteria.The resistance rate of Streptococcus pneumoniae to Penicillin was 3.9% .All antibiotics excepted cotrimoxazole remained highly active against the haemophilus influenzae.All antibiotics except macrolide anti-biotics remained highly active against the moraxella catarrhalis.Conclusion Penicillin-insensitive Streptococcus pneumoniae,mac-rolides-resistant gram-positive cocci,cephalosporin-resistant Enterobacteriaceae ,Oxacillin-resistance coagulase-negative staphylo-cocci were revealed to be the most serious problems in terms of bacteria resistance for children in Sichuan province.

BACKGROUND: Polypyrrole (PPy) has been widely applied in biomedical fields due to its special electronic property. Past 20 years, there are an increasing number of studies on the application of PPy as a potentially electrically addressable tissue/cell support substrate for tissue/cell regeneration.OBJECTIVE: To overall review the application of PPy in tissue engineering field, and to provide a new approach for the research and development of medical biomaterials.METHODS: Pubmed and Chinese biomedicine literature database were searched using key word of "polypyrrole" for documents published between 1990 and 2010. Literatures related to application of PPy in tissue engineering field were included, and the repetitive articles were excluded.RESULTS AND CONCLUSION: Totally 762 papers were searched by computer, according to the inclusive and exclusive criteria, 51 literature were reviewed. Currently, PPy has been widely used in the fields of cardiovascular tissue engineering, bone and muscle tissue engineering, as well as skin tissue engineering. Application of PPy and PPy-based biomaterials hold great potential in development of novel biomedical materials applied in tissue engineering due to their versatile functionality and superior biocompatibility.

Objective To summarize the cause and the level change of laboratory indicators in children with hemphagocytic lym‐phohistiocytosis(HLH) .Methods Retrospectively analyze the cause and the diagnose features of the 13 HLH patients .Results The first symptom of the desease was hyperpyrexia as well as the hepatosplenomegaly and the lymphadenectasis .Then it went with the peripheral cytopenia in two or three lineages ,different degrees of the liver dysfunction and the coagulation abnormalities .The number of histiocytes increased and hemophagocytic macrophages were found in bone marrow .It also showed that the elevated ser‐um triglycerides ,serum ferritin ,lactate dehydrogenase and glutamic‐pyruvic transaminase and decreased fibrinogen in laboratory findings .Conclusion Cytopenia(in two or three lineages ,especially the platelet) ,the elevated serum triglycerides ,serum ferritin , lactate dehydrogenase ,glutamic‐pyruvic transaminase ,decreased fibrinogen and the hemophagocytic macrophages in bone marrow (≥1% ) are important indicators in hemphagocytic lymphohistiocytosis diagnosis ,which could help us know the change of the HLH laboratory features better and diagnose the disease correctly .

Objective To investigate the diagnostic values of vaginitis five of the joint inspection kit combined with microscopic examination for common vaginal disease.Methods The vaginal secretions samples from 4 114 outpatients were tested with LTS-V400 vaginitis five of the joint inspection kit and microscopic examination.The examination results of age groups were analyzed and compared.Results In all 4 114 cases of samples,the positive rate of trichomonad(1.95%)was significantly lower than that of fun-gi(4.74%),P <0.05.The positive rates of N-acetyl-beta-galactosamine glycosides enzyme(NAG),sialidase(SNA),and leukocyte esterase(LE)were the highest in >40-50-age group,which were 1.95%,6.10%,14.15%,10.24% and 46.34%,respectively. The positive rates of H2 O2 ,pH >4.5 and pH < 3.8 were the highest in > 50 age group,which were 85.43%,86.09%,and 0.66%,respectively.The positive rates of trichomonad,SNA,LE,H2 O2 ,pH>4.5 and pH<3.8 were statistically different a-mong the age groups(P <0.05 ).Conclusion Vaginitis five of the joint inspection kit combined with microscopic examination is suitable for the diagnosis of trichomonas vaginitis,mouldsex vaginitis,and bacterial vaginal disease .

Objective:To prepare mesalazineβ-cyclodextrin inclusion compound and observe its properties. Methods:The struc-ture was characterized by UV, IR and DSC. The inclusion process of mesalazine andβ-cyclodextrin was simulated using molecular doc-king technique. The solubility and dissolution of inclusion compound were determined. Results:UV, IR and DSC were used to deter-mine the formation of inclusion complex. The inclusion ratio was 1: 1 and the phase solubility diagram was AL type. The solubility of inclusion compound in deionized water (25 ℃, pH 7. 0) was 7. 8 mg·ml-1 . The preparation of mesalazine β-cyclodextrin inclusion compound could significantly increase the dissolution rate and solubility of mesalazine. Conclusion:The prepared mesalazineβ-cyclo-dextrin inclusion compound can obviously improve the poor water solubility and dissolution of mesalazine.