Objective To observe the safety and severity of heat-sensitive moxibustion in treating poisonous snakebites-induced bladder dysfunction of different degrees.Method Twenty-six patients diagnosed with bladder dysfunction due to poisonous snakebites were examined by type-B ultrasonic scanning for residual urine volume and uroflowmetry. The patients were treated with heat-sensitive moxibustion. The residual urine volume and uroflowmetry parameters were compared between different severity degrees of bladder dysfunction before and after treatment.Result The residual urine volumes were changed significantly in the mild group and moderate group after treatment (P<0.01); the residual urine volume showed insignificant change after treatment in the severe group (P>0.05). The maximum urine flow rate (Qmax) was significantly changed in the mild group after the treatment (P<0.05); the Qmax was insignificantly changed in the moderate and severe groups after treatment (P>0.05).Conclusion Heat-sensitive moxibustion is significantly effective in treating mild-moderate bladder dysfunction due to poisonous snakebites, and it’s safe and reliable.

Objective To observe the curative effect of combined with small dose of thalidomide and low -dose dexamethasone VAD regimen for the elderly multiple myeloma (MM).Methods 58 MM cases were randomly divided into control group(28 cases)and treatment group (30 cases).The control group received VAD regimen.The treatment group added with small doses of thalidomide treatment (TVAD scheme).The,hemoglobin (HGB),creati-nine (Cr),bone marrow plasma cell counting,M protein level,Carr's score (KPS)and side effects of chemotherapy were observed in the two groups.Results The effective rates of treatment group and control group were 76.67% and 50.00%,the curative effect of treatment group was better (χ2 =4.459,P <0.05).The HGB,Cr,plasma cells,Mpro-tein and carr's score were improved,but the curative effect of the treatment group was better(P <0.05).Adverse reac-tions such as leucopenia,neuropathy,constipation,gastrointestinal reaction and pulmonary infection were observed in two groups,but there was no significant difference.Conclusion TVAD regimen for MMhas good curative effect and less side effects,it is worthy of promotion.

Objective To investigate the effect of anti thymic globulin (ATG)combined with cyclosporine (CSA)immunosuppressive therapy in patients with severe aplastic anemia (SAA).Methods 20 patients with SAA were treated with CSA combined with immunosuppressive therapy,and the changes of blood indexes after treatment were observed.Results After treatment,4 cases (13.33%)were cured,11 cases (36.67%)were relieved,8 cases (26.67%)were improved,7 cases (23.33%)were ineffective,the total effective rate was 76.67%.After the treatment of blood routine,examination of Hb,WBC and PLT treatment 6 months compared with before treatment,the difference were statistically significant (t =5.678,3.675,7.685,all P <0.05).Patients after treatment,1 case had allergic reaction,which accounted for 3.33%,and was finally given the glucocorticoid,then improved.1 case had extremely low WBC,accounting for 3.33%,appeared serious infection and death after testing.Conclusion The effect of ATG combined with CSA immunosuppression in the treatment of SAA is effective obviously,and it has a certain safety,vwhich is worthy of clinical recommendation.

Objective To discuss the safety and effectiveness of heat-sensitive moxibustion for bladder dysfunction after poisonous snakebite. Method Twenty-six patients with bladder dysfunction due to poisonous snakebite were intervened by heat-sensitive moxibustion, and the changes of residual urine volume and uroflowmetry parameters were analyzed by using type-B ultrasonic scanning and uroflowmetry. Result According to the ultrasonic scanning and uroflowmetry, the residual urine volume was significantly changed after treatment in the 26 subjects (P<0.05), and the maximum urine flow rate (Qmax) was also significantly different from that before treatment (P<0.05). Conclusion Heat-sensitive moxibustion can improve the residual urine volume and Qmax in bladder dysfunction after poisonous snakebite, and it’s a safe and reliable method.

Objective To investigate the effects of aquaporin 3 (AQP3) and phospholipase D2 (PLD2) on the proliferation and apoptosis of a human cutaneous squamous cell carcinoma cell line A431.Methods Three small interfering RNAs (siRNAs) were constructed targeting the AQP3 and PLD2 genes separately,and transfected into A431 cells using liposomes.Then,fluorescence quantitative PCR was performed to find the most efficient siRNAs.Western blot was conducted to detect the protein expression levels of AQP3 and PLD2 in A431 cells after transfection with the selected AQP3-siRNA and PLD2-siRNA.Some A431 cells were divided into five groups:normal control group without any treatment,transfection reagent group treated with the oligofectamine reagent only,negative control group transfected with the negative control siRNA,AQP3-siRNA group transfected with the selected AQP3-siRNA,PLD2-siRNA group transfected with the selected PLD2-siRNA.After additional culture,cell counting kit-8 assay was performed to evaluate the proliferation of A431 cells,flow cytometry to detect the apoptosis of A431 cells after annexin V-fluorescein isocyanate/propidium iodide double-staining.Statistical analysis was carried out by the paired t test.Results The transfection with AQP3-siRNA and PLD2-siRNA induced a significant decrease in the mRNA and protein expressions of AQP3 and PLD2 respectively in A431 cells when compared with the untransfected cells.Compared with the negative control group,the proliferation of A431 cells was significantly decelerated at 24,48 and 72 hours after transfection in the AQP3-siRNA group (t =24.10,11.00,9.54,respectively,all P ＜ 0.01) and PLD2-siRNA group (t =30.47,7.02,8.73,respectively,all P ＜ 0.01).A significant increase was observed in the apoptosis of A431 cells at 48 and 72 hours after transfection with AQP3-siRNA (t =11.36,20.91,respectively,both P ＜ 0.01),and at 72 hours after transfection with PLD2-siRNA (t =4.86,P ＜ 0.05) compared with the negative control group.Conclusion The down-regulation of AQP3 and PLD2 expressions by siRNA can inhibit the proliferation,but induce the apoptosis,of A431 cells.

Objective To explore the current status of traditional Chinese medicine(TCM)research on alopecia areata(AA)and arogenetic alopecia(AGA),analyzing the clinical syndrome and treatment characteristics,and providing reference for TCM clinical diagnosis and treatment.Methods Search for research literature on traditional Chinese medicine treatment of hair loss in CNKI,Wanfang,and VIP separately.Removing duplicates through Notexpress and using CiteSpace and VOSviewer software for bibliometric analysis.Furthermore,focusing on the RCT research of traditional Chinese medicine internal treatment for AA and AGA,the data mining methods were used to analyze the pathogenesis,treatment methods,and prescriptions of traditional Chinese medicine.Results There are a total of 2954 literature on the treatment of hair loss by traditional Chinese medicine,with clinical research being the main research type.Among them,the main pathogenesis of AA is liver and kidney deficiency,qi and blood deficiency,etc.Commonly used herbs includes Polygonum multiflorum,Rehmannia glutinosa,Chuanxiong,Poria cocos,and Ligustrum lucidum,while the pathogenesis of AGA is mainly dampness heat accumulation,yin deficiency dampness heat,etc.,with commonly used herbs such as Platycladus orientalis,Alisma orientalis,Poria cocos,hawthorn,and Coix seed.Meanwhile,AA and AGA both pay more attention to the use of tonifying drugs in their treatment.Conclusion There is a growing trend in literature on the treatment of hair loss with TCM,and there are differences in the pathogenesis and treatment methods between AA and AGA in traditional Chinese medicine.It is worth further reference in the prescription and medication of clinical treatment of hair loss.

Objective To explore the clinical efficacy of hyperbaric oxygen combined with Sheshang powder in the treatment of venomous snake bite.Methods A total of 112 patients with venomous snake bite admitted to Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University from April 2021 to October 2023 were selected,and they were divided into conventional treatment group,conventional treatment+Sheshang powder treatment group,conventional treatment+hyperbaric oxygen treatment group and conventional treatment+hyperbaric oxygen combined with Sheshang powder treatment group according to random number table method,with 28 cases in each group.The difference of circumference between injured limb and healthy limb,visual analogue scale(VAS)score and coagulation function index[prothrombin time(PT),activated partial thromboplastin time(APTT),fibrinogen(Fib)]of four groups were compared.Results After treatment,the swelling of injured limbs in four groups gradually decreased over time(P＜0.05).On the third day of treatment,the circumference difference of patients in conventional treatment+hyperbaric oxygen combined with Sheshang powder treatment group was significantly lower than that in conventional treatment group and conventional treatment+Sheshang powder treatment group(P＜0.05).After treatment,PT,APTT and Fib of four groups were better than before treatment(P＜0.05),PT and APTT of patients in conventional treatment+Sheshang powder treatment group were significantly lower than those in conventional treatment group and conventional treatment+hyperbaric oxygen treatment group,and Fib was significantly higher than that in conventional treatment group and conventional treatment+hyperbaric oxygen treatment group(P＜0.05).PT and APTT of patients in conventional treatment+hyperbaric oxygen combined with Sheshang powder treatment group were significantly lower than those in other three groups,and Fib was significantly higher than that in other three groups(P＜0.05).After treatment,the VAS scores of four groups were significantly lower than before treatment(P＜0.05),and there were significant differences in VAS scores among the four groups(P＜0.05).Conclusion Hyperbaric oxygen combined with Sheshang powder can effectively improve the coagulation function of patients bitten by venomous snakes,relieve limb swelling and pain.

To determine the contents of oxypeucedanin, oxypeucedanin hydrate, byakangelicol and byak-angelicin both before and after Angelicae Dahuricae Radix was stewed with yellow rice wine by high-performance liquid chromatography, and study the mutual transformation mechanisms of oxypeucedanin into oxypeucedanin hydrate, as well as byakangelicol into byak-angelicin. The research results indicated that the contents of oxypeucedanin and byakangelicol were decreased, but the contents of oxypeucedanin hydrate and byak-angelicin were increased after Angelicae Dahuricae Radix was processed with yellow rice wine. The contents' changes of these chemical compounds were due to the ring opening reaction of epoxy compounds, such as oxypeucedanin and byakangelicol under the weak acidity and heating conditions of yellow rice wine. This research could provide a scientific basis for the processing mechanism of Angelicae Dahuricae Radix with yellow rice wine stewing.