Objective To investigate the expression of ER,PR,HER-2,PCNA and P53 in breast cancer patients in Shanghai and the relevant clinical significance.Methods Expressions of ER,PR,HER-2,PCNA and P53 in the breast cancer tissues of 544 patients in Shanghai were detected by immunohistochemistry methods.Statistical analysis was applied to analyze the relationship of these immunohistochemical indices with the clinicopathological features of breast cancer.Results The positive rates of ER,PR,HER-2,PCNA and P53 in the breast cancer tissues of 544 patients were 62.2%,57.2%,15.1%,82.6% and 58.5%,respectively.The expressions of ER,PCNA and P53 were correlated with the tumor size(P

Purpose To detect the expression profile of microRNAs ( miRNAs) in human breast cancer cells BT474 and BT474/ siR-NA HER-2, and to screen out miRNA expression profile related to HER-2. Methods RNA interference was used to inhibit HER-2 expression in human breast cancer line BT474. The expression profile of miRNAs was detected by miRNA microarray technique. Re-sults According to discrepancy power >2. 0 and P value<0. 05, there were 338 miRNAs related to HER-2, including 110 up-regu-lated and 228 down-regulated miRNAs. Conclusion miRNA microarrays can detect the differentially expressed miRNAs in human breast cancer BT474 cells with different level of HER-2. The miRNAs related to HER-2 which play a role in breast cancer should be found after further analysis.

Purpose To screen out miRNA expression profile related to HER-2 via observing the differential expression of miRNA ex-pression of human breast cancer cells MCF-7 and MCF-7/HER-2. Methods After cultivating human breast cancer cells MCF-7 and MCF-7/HER-2, the expression profile of miRNAs was detected by miRNA microarrays. Results Compared to MCF-7 cells, two hundreds and seventeen HER-2 related miRNAs were identified from breast cancer MCF-7/HER-2 cells, including 123 up-regulated and 94 down-regulated miRNAs. Of significant differentialy expressed miRNAs, hsa-miR-141 and hsa-miR-1299’s target genes have multiple biological functions. Conclusion The differentially expressed miRNAs under different lever of HER-2 in human breast cancer MCF-7 cells were obtained, which may provide a basis for studying HER-2 functions and roles in breast cancer.

Objective:To evaluate the clinical efficacy of subgingival irrigation with chlorhexidine as an adjunct to ultrasonic subgingival scaling for the treatment of moderate to severe chronic periodontitis in elderly patients.Methods:Twenty-two elderly patients diagnosed with moderate to severe chronic periodontitis were randomly assigned to test and control groups based on mouth side.In the test group, 0.02% chlorhexidine was used in place of distilled water for irrigation during ultrasonic scaling, while the control group received routine ultrasonic subgingival scaling.The plaque index(PLI), probing depth(PD), clinical attachment level(CAL), gingival bleeding index(BI), and percentage of bleeding on probing(BOP)were recorded at baseline, 6 weeks post-treatment, and 3 months post-treatment.Results:At 6 weeks and 3 months post-treatment, both the test and control sides exhibited a significant reduction in PD from baseline(both P<0.05).However, the magnitude of change was comparable between the two sides, with no statistically significant difference observed( P=0.591, 0.106).Both sides also demonstrated statistically significant differences in CAL at 6 weeks and 3 months compared to baseline(both P<0.05).Although the change in the test side was slightly greater than that in the control side, this difference was not statistically significant( P=0.758, 0.909).Regarding BI, both sides showed a significant difference from baseline at both 6 weeks and 3 months(both P<0.05), with statistically significant differences between the test and control groups( P=0.003, 0.015), indicating a significantly better improvement in gingival bleeding on the test side.Similarly, both sides demonstrated improvement in BOP at 6 weeks and 3 months, with the test side showing a significantly better improvement than the control side( P=0.005, 0.019).The PLI also showed statistically significant differences compared to baseline at 6 weeks and 3 months(both P<0.05); however, there was no statistically significant difference between the two sides(both P>0.05). Conclusions:The use of ultrasonic scalers in conjunction with chlorhexidine for subgingival scaling and root planning can significantly enhance pocket depth reduction and decrease gingival bleeding in elderly patients suffering from chronic periodontitis.When compared to traditional treatment methods, the therapeutic outcomes of ultrasonic scalers combined with chlorhexidine are superior and more favorable for the elderly population.