Objective To establish a high purity primary culture methods of human nasal epithelial cells (HNEC) in vitro and to provide a successful primary culture model for evaluation experiments of the nasal preparation .Methods Primary culture of human nasal epithelial cells were performed with enzymatic dissociation of isolated tissue and cultured in serum-free medium .HNEC were separated through magnetic field by immunomagnetic beads .We determined the purity of the separated cells by light microscopy and flow cytometry .The morphology of HNEC was observed with a scanning electron microscope .Results Under an inverted phase microscope ,the cells morphology was paving stone shaped .Under the scanning electron microscopy ,abundant microvilli and cilia differentiation were observed .Flow cytometry showed the epithelial cells accounted for 99% .Conclusion The highly purified HNEC can be directly isolated by the magnetic cell sorting system .The cell model can be used for the basic research of nasal cavity preparation .

Objective:To investigate the characteristics and influencing factors of bacterial infection around dental implants(BIADI).Methods:From December 2018 to December 2019, 300 patients with oral implanting in the First People's Hospital of Wenling were selected as the observation objects, among them 43 patients had bacterial infection around the implants, with the incidence rate of 14.33%.The control group (257 cases) had no infection and the observation group with infection.The samples of gingival crevicular floor of patients with BIADI were collected and the pathogens were isolated and identified by the bioassay instrument of French merrier biological company.Single factor analysis was used to analyze the related factors of BIADI, and multi-factor analysis was used to analyze the independent risk factors of BIADI.Results:In 43 patients with BIADI, 46 strains of pathogenic bacteria were isolated, which were mainly anaerobes 30 strains(65.22%), and 11 strains were aerobes (23.91%). Single factor analysis showed that there were no significant differences between the two groups in gender, education level, history of chronic periodontitis, oral health habits and residence (all P>0.05). There were statistically significant differences between the two groups in age, combined with basic diseases, smoking history and alveolar bone around implants (all P<0.05). The results showed that age>60 years old, combined with basic diseases, smoking history and alveolar bone defects were independent risk factors for bacterial infection. Conclusion:The main pathogens of BIADI are anaerobes, and the infection is affected by many factors, including age, basic diseases, smoking history and alveolar bone defects around dental implants.

Objective To study the optimum technological conditions of macroporous resin separation and purification of polyphenol composition. Methods Fingerprint of Ilex paraguariensis was established by HPLC method. The best resin, loading conditions and elution requirements were screened with chlorogenic acid and elution rate of total active constituents served as the index. Results The optimum technological conditions were as follows:the separation carrier was D101 macroporous resin,the concentration of the sample liquid was 0.10 g?mL-1 ,the resin adsorption quantity of crude drugs was 1.5 g?g-1 ,the impurity was removed by four times of amount of water at first,and then eluted with four times the amount of 70％ ethanol in a gradient manner.After purification,the elution rate of chlorogenic acid was up to 82.83％,and the elution rate of total effective component was about 72. 48％. The resemblance of HPLC fingerprint was over 0. 98, and the components were balancedly recoveried. Conclusion The purification process of total polyphenols in Ilex paraguariensis by D101 macroporous resin was simple,reliable,and can be used for large-scale production.

Lung cancer is the leading cause of cancer death worldwide as well as in China. For many years, conventional oncologic treatments such as surgery, chemotherapy, and radiotherapy (RT) have dominated the field of non-small cell lung cancer (NSCLC). The recent introduction of immunotherapy in clinical practice, led to a paradigm shift in lung cancer as in many other solid tumors. Recent pre-clinical and clinical data have shown RT may also modify antitumor immune responses through induction of immunogenic cell death and reprogramming of the tumor microenvironment. This has led many to reexamine RT as a partner therapy to immuno-oncology treatments and investigate their potential synergy in an exponentially growing number of clinical trials. Clinical trials combining radiotherapy and immunotherapy are attracting major attention, experts were invited to discuss frontier and controversial academic topics: (1) Recent developments of clinical synergy between radiation and immune checkpoint inhibitors (ICIs) in the treatment of NSCLC; (2) Will immunotherapy and radiotherapy increase the toxicity risk for cancer patients; (3) How to cope the mixed responses/disassociated responses phenomenon in checkpoint inhibition therapy to NSCLC with local ablative therapy; (4) Combining radiotherapy and immunotherapy in the treatment of NSCLC brain metastases.